Next Generation Sequencing of miRNAs – Strategies, Resources and Methods

miRNAs constitute a family of small RNA species that have been demonstrated to play a central role in regulating gene expression in many organisms. With the advent of next generation sequencing, new opportunities have arisen to identify and quantify miRNAs and elucidate their function. The unprecede...

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Detalles Bibliográficos
Autores principales: Motameny, Susanne, Wolters, Stefanie, Nürnberg, Peter, Schumacher, Björn
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2010
Materias:
Review
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3960865/
https://www.ncbi.nlm.nih.gov/pubmed/24710011
http://dx.doi.org/10.3390/genes1010070
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author Motameny, Susanne
Wolters, Stefanie
Nürnberg, Peter
Schumacher, Björn
author_facet Motameny, Susanne
Wolters, Stefanie
Nürnberg, Peter
Schumacher, Björn
author_sort Motameny, Susanne
collection PubMed
description miRNAs constitute a family of small RNA species that have been demonstrated to play a central role in regulating gene expression in many organisms. With the advent of next generation sequencing, new opportunities have arisen to identify and quantify miRNAs and elucidate their function. The unprecedented sequencing depth reached by next generation sequencing technologies makes it possible to get a comprehensive miRNA landscape but also poses new challenges for data analysis. We provide an overview of strategies used for miRNA sequencing, public miRNA resources, and useful methods and tools that are available for data analysis.
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spelling pubmed-39608652014-03-26 Next Generation Sequencing of miRNAs – Strategies, Resources and Methods Motameny, Susanne Wolters, Stefanie Nürnberg, Peter Schumacher, Björn Genes (Basel) Review miRNAs constitute a family of small RNA species that have been demonstrated to play a central role in regulating gene expression in many organisms. With the advent of next generation sequencing, new opportunities have arisen to identify and quantify miRNAs and elucidate their function. The unprecedented sequencing depth reached by next generation sequencing technologies makes it possible to get a comprehensive miRNA landscape but also poses new challenges for data analysis. We provide an overview of strategies used for miRNA sequencing, public miRNA resources, and useful methods and tools that are available for data analysis. MDPI 2010-06-03 /pmc/articles/PMC3960865/ /pubmed/24710011 http://dx.doi.org/10.3390/genes1010070 Text en © 2010 by the authors; licensee MDPI, Basel, Switzerland http://creativecommons.org/licenses/by/3.0/ This article is an Open Access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Review
Motameny, Susanne
Wolters, Stefanie
Nürnberg, Peter
Schumacher, Björn
Next Generation Sequencing of miRNAs – Strategies, Resources and Methods
title Next Generation Sequencing of miRNAs – Strategies, Resources and Methods
title_full Next Generation Sequencing of miRNAs – Strategies, Resources and Methods
title_fullStr Next Generation Sequencing of miRNAs – Strategies, Resources and Methods
title_full_unstemmed Next Generation Sequencing of miRNAs – Strategies, Resources and Methods
title_short Next Generation Sequencing of miRNAs – Strategies, Resources and Methods
title_sort next generation sequencing of mirnas – strategies, resources and methods
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3960865/
https://www.ncbi.nlm.nih.gov/pubmed/24710011
http://dx.doi.org/10.3390/genes1010070
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