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Downregulation of L1CAM inhibits proliferation, invasion and arrests cell cycle progression in pancreatic cancer cells in vitro

The aim of the present study was to establish the effect of silencing L1 cell adhesion molecule (L1CAM) on the proliferation, invasion, cell cycle progression and apoptosis of pancreatic cancer cells, and to determine the potential molecular mechanisms that are involved. The human Capan-2 pancreatic...

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Autores principales: BEN, QIWEN, AN, WEI, FEI, JIAN, XU, MAOJIN, LI, GUIXIANG, LI, ZHAOSHEN, YUAN, YAOZONG
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3961134/
https://www.ncbi.nlm.nih.gov/pubmed/24660028
http://dx.doi.org/10.3892/etm.2014.1519
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author BEN, QIWEN
AN, WEI
FEI, JIAN
XU, MAOJIN
LI, GUIXIANG
LI, ZHAOSHEN
YUAN, YAOZONG
author_facet BEN, QIWEN
AN, WEI
FEI, JIAN
XU, MAOJIN
LI, GUIXIANG
LI, ZHAOSHEN
YUAN, YAOZONG
author_sort BEN, QIWEN
collection PubMed
description The aim of the present study was to establish the effect of silencing L1 cell adhesion molecule (L1CAM) on the proliferation, invasion, cell cycle progression and apoptosis of pancreatic cancer cells, and to determine the potential molecular mechanisms that are involved. The human Capan-2 pancreatic cancer cell line was infected with lentivirus-mediated short hairpin RNA (shRNA) to target L1CAM. Cell proliferation and invasion were analyzed using cell counting kit-8 and Transwell assays, respectively, and cell cycle progression and apoptosis were analyzed using flow cytometry. L1CAM protein expression in Capan-2 cells decreased following shRNA-L1CAM infection. Furthermore, knockdown of L1CAM significantly inhibited cell proliferation and reduced the number of invasive cells, while increasing the percentage of cells in the G0/G1 phase (P<0.05). However, the effect on apoptosis was not identified to be statistically significant. In addition, L1CAM silencing may induce activation of p38/extracellular signal regulated kinase 1/2. Downregulation of L1CAM may inhibit proliferation, invasion and arrests cell cycle progression in pancreatic cancer via p38/ERK1/2 signal pathway, and therefore, L1CAM may serve as a potential target for gene therapy in pancreatic cancer.
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spelling pubmed-39611342014-03-21 Downregulation of L1CAM inhibits proliferation, invasion and arrests cell cycle progression in pancreatic cancer cells in vitro BEN, QIWEN AN, WEI FEI, JIAN XU, MAOJIN LI, GUIXIANG LI, ZHAOSHEN YUAN, YAOZONG Exp Ther Med Articles The aim of the present study was to establish the effect of silencing L1 cell adhesion molecule (L1CAM) on the proliferation, invasion, cell cycle progression and apoptosis of pancreatic cancer cells, and to determine the potential molecular mechanisms that are involved. The human Capan-2 pancreatic cancer cell line was infected with lentivirus-mediated short hairpin RNA (shRNA) to target L1CAM. Cell proliferation and invasion were analyzed using cell counting kit-8 and Transwell assays, respectively, and cell cycle progression and apoptosis were analyzed using flow cytometry. L1CAM protein expression in Capan-2 cells decreased following shRNA-L1CAM infection. Furthermore, knockdown of L1CAM significantly inhibited cell proliferation and reduced the number of invasive cells, while increasing the percentage of cells in the G0/G1 phase (P<0.05). However, the effect on apoptosis was not identified to be statistically significant. In addition, L1CAM silencing may induce activation of p38/extracellular signal regulated kinase 1/2. Downregulation of L1CAM may inhibit proliferation, invasion and arrests cell cycle progression in pancreatic cancer via p38/ERK1/2 signal pathway, and therefore, L1CAM may serve as a potential target for gene therapy in pancreatic cancer. D.A. Spandidos 2014-04 2014-02-05 /pmc/articles/PMC3961134/ /pubmed/24660028 http://dx.doi.org/10.3892/etm.2014.1519 Text en Copyright © 2014, Spandidos Publications http://creativecommons.org/licenses/by/3.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.
spellingShingle Articles
BEN, QIWEN
AN, WEI
FEI, JIAN
XU, MAOJIN
LI, GUIXIANG
LI, ZHAOSHEN
YUAN, YAOZONG
Downregulation of L1CAM inhibits proliferation, invasion and arrests cell cycle progression in pancreatic cancer cells in vitro
title Downregulation of L1CAM inhibits proliferation, invasion and arrests cell cycle progression in pancreatic cancer cells in vitro
title_full Downregulation of L1CAM inhibits proliferation, invasion and arrests cell cycle progression in pancreatic cancer cells in vitro
title_fullStr Downregulation of L1CAM inhibits proliferation, invasion and arrests cell cycle progression in pancreatic cancer cells in vitro
title_full_unstemmed Downregulation of L1CAM inhibits proliferation, invasion and arrests cell cycle progression in pancreatic cancer cells in vitro
title_short Downregulation of L1CAM inhibits proliferation, invasion and arrests cell cycle progression in pancreatic cancer cells in vitro
title_sort downregulation of l1cam inhibits proliferation, invasion and arrests cell cycle progression in pancreatic cancer cells in vitro
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3961134/
https://www.ncbi.nlm.nih.gov/pubmed/24660028
http://dx.doi.org/10.3892/etm.2014.1519
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