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Direct Observation of Unstained Biological Specimens in Water by the Frequency Transmission Electric-Field Method Using SEM
Scanning electron microscopy (SEM) is a powerful tool for the direct visualization of biological specimens at nanometre-scale resolution. However, images of unstained specimens in water using an atmospheric holder exhibit very poor contrast and heavy radiation damage. Here, we present a new form of...
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Public Library of Science
2014
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3961424/ https://www.ncbi.nlm.nih.gov/pubmed/24651483 http://dx.doi.org/10.1371/journal.pone.0092780 |