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Association between mitochondrial C-tract alteration and tobacco exposure in oral precancer cases
INTRODUCTION: Tobacco exposure is a known risk factor for oral cancer. India is home to oral cancer epidemic chiefly due to the prevalent use of both smoke and smokeless tobacco. To reduce the related morbidity early detection is required. The key to this is detailing molecular events during early p...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Medknow Publications & Media Pvt Ltd
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3961899/ https://www.ncbi.nlm.nih.gov/pubmed/24665180 http://dx.doi.org/10.4103/0975-5950.127655 |
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author | Pandey, Rahul Mehrotra, Divya Mahdi, Abbas Ali Sarin, Rajiv Kowtal, Pradnya Maurya, Shailendra S. Parmar, Devendra |
author_facet | Pandey, Rahul Mehrotra, Divya Mahdi, Abbas Ali Sarin, Rajiv Kowtal, Pradnya Maurya, Shailendra S. Parmar, Devendra |
author_sort | Pandey, Rahul |
collection | PubMed |
description | INTRODUCTION: Tobacco exposure is a known risk factor for oral cancer. India is home to oral cancer epidemic chiefly due to the prevalent use of both smoke and smokeless tobacco. To reduce the related morbidity early detection is required. The key to this is detailing molecular events during early precancer stage. Mitochondrion is an important cellular organelle involved in cell metabolism and apoptosis. Mitochondrial dysfunction is thought to be the key event in oncogenesis. Last decade has seen a spurt of reports implicating mitochondrial mutations in oral carcinogenesis. However, there are few reports that study mitochondrial deoxyribonucleic acid (mtDNA) changes in oral precancer. This study aims to understand and link effect of tobacco exposure on mtDNA in oral precancer cases. SUBJECTS AND METHODS: A total of 100 oral precancer cases of which 50 oral leukoplakia and 50 oral submucous fibrosis were recruited in the study and a detailed questionnaire were filled about the tobacco habits. Their tissue and blood samples were collected. Total genomic DNA was isolated from both sources. Mitochondrial C-tract was amplified and bidirectional sequencing was carried out. Mutations were scored over matched blood DNA. RESULTS: There was a significant association between the presence of mitochondrial C-tract alteration and duration of tobacco exposure. The probability increased with increasing duration of tobacco consumption. The risk of having this alteration was more in chewers than in smokers. CONCLUSIONS: Tobacco in both form, chewable and smoke, is oncogenic and causes early changes in mitochondrial genome and chances increases with increasing duration of tobacco consumption. |
format | Online Article Text |
id | pubmed-3961899 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Medknow Publications & Media Pvt Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-39618992014-03-24 Association between mitochondrial C-tract alteration and tobacco exposure in oral precancer cases Pandey, Rahul Mehrotra, Divya Mahdi, Abbas Ali Sarin, Rajiv Kowtal, Pradnya Maurya, Shailendra S. Parmar, Devendra Natl J Maxillofac Surg Original Article INTRODUCTION: Tobacco exposure is a known risk factor for oral cancer. India is home to oral cancer epidemic chiefly due to the prevalent use of both smoke and smokeless tobacco. To reduce the related morbidity early detection is required. The key to this is detailing molecular events during early precancer stage. Mitochondrion is an important cellular organelle involved in cell metabolism and apoptosis. Mitochondrial dysfunction is thought to be the key event in oncogenesis. Last decade has seen a spurt of reports implicating mitochondrial mutations in oral carcinogenesis. However, there are few reports that study mitochondrial deoxyribonucleic acid (mtDNA) changes in oral precancer. This study aims to understand and link effect of tobacco exposure on mtDNA in oral precancer cases. SUBJECTS AND METHODS: A total of 100 oral precancer cases of which 50 oral leukoplakia and 50 oral submucous fibrosis were recruited in the study and a detailed questionnaire were filled about the tobacco habits. Their tissue and blood samples were collected. Total genomic DNA was isolated from both sources. Mitochondrial C-tract was amplified and bidirectional sequencing was carried out. Mutations were scored over matched blood DNA. RESULTS: There was a significant association between the presence of mitochondrial C-tract alteration and duration of tobacco exposure. The probability increased with increasing duration of tobacco consumption. The risk of having this alteration was more in chewers than in smokers. CONCLUSIONS: Tobacco in both form, chewable and smoke, is oncogenic and causes early changes in mitochondrial genome and chances increases with increasing duration of tobacco consumption. Medknow Publications & Media Pvt Ltd 2013 /pmc/articles/PMC3961899/ /pubmed/24665180 http://dx.doi.org/10.4103/0975-5950.127655 Text en Copyright: © National Journal of Maxillofacial Surgery http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Pandey, Rahul Mehrotra, Divya Mahdi, Abbas Ali Sarin, Rajiv Kowtal, Pradnya Maurya, Shailendra S. Parmar, Devendra Association between mitochondrial C-tract alteration and tobacco exposure in oral precancer cases |
title | Association between mitochondrial C-tract alteration and tobacco exposure in oral precancer cases |
title_full | Association between mitochondrial C-tract alteration and tobacco exposure in oral precancer cases |
title_fullStr | Association between mitochondrial C-tract alteration and tobacco exposure in oral precancer cases |
title_full_unstemmed | Association between mitochondrial C-tract alteration and tobacco exposure in oral precancer cases |
title_short | Association between mitochondrial C-tract alteration and tobacco exposure in oral precancer cases |
title_sort | association between mitochondrial c-tract alteration and tobacco exposure in oral precancer cases |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3961899/ https://www.ncbi.nlm.nih.gov/pubmed/24665180 http://dx.doi.org/10.4103/0975-5950.127655 |
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