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Prmt7 is dispensable in tissue culture models for adipogenic differentiation

Protein arginine methylation is a common posttranslational modification that has been implicated in numerous biological processes including gene expression. The mammalian genome encodes nine protein arginine methyltransferases (Prmts) that catalyze monomethylation, asymmetric dimethylation, and symm...

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Autores principales: Hu, Yu-Jie, Sif, Saïd, Imbalzano, Anthony N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: F1000Research 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3962006/
https://www.ncbi.nlm.nih.gov/pubmed/24715966
http://dx.doi.org/10.12688/f1000research.2-279.v1
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author Hu, Yu-Jie
Sif, Saïd
Imbalzano, Anthony N.
author_facet Hu, Yu-Jie
Sif, Saïd
Imbalzano, Anthony N.
author_sort Hu, Yu-Jie
collection PubMed
description Protein arginine methylation is a common posttranslational modification that has been implicated in numerous biological processes including gene expression. The mammalian genome encodes nine protein arginine methyltransferases (Prmts) that catalyze monomethylation, asymmetric dimethylation, and symmetric dimethylation on arginine residues. Protein arginine methyltransferase 7 (Prmt7) is categorized as a type II and type III enzyme that produces symmetric dimethylated arginine and monomethylated arginine, respectively. However, the biological role of Prmt7 is not well characterized. We previously showed that Prmt5, a type II Prmt that associates with Brg1-based SWI/SNF chromatin remodeling complex, is required for adipocyte differentiation. Since Prmt7 also associates with Brg1-based SWI/SNF complex and modifies core histones, we hypothesized that Prmt7 might play a role in transcriptional regulation of adipogenesis. In the present study, we determined that the expression of Prmt7 did not change throughout adipogenic differentiation of C3H10T1/2 mesenchymal cells. Knockdown or over-expression of Prmt7 had no effect on lipid accumulation or adipogenic gene expression in differentiating C3H10T1/2 cells or in C/EBPα-reprogrammed NIH3T3 fibroblasts. Based on these results, we conclude that Prmt7, unlike Prmt5, is dispensable for adipogenic differentiation in tissue culture models.
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spelling pubmed-39620062014-04-07 Prmt7 is dispensable in tissue culture models for adipogenic differentiation Hu, Yu-Jie Sif, Saïd Imbalzano, Anthony N. F1000Res Research Article Protein arginine methylation is a common posttranslational modification that has been implicated in numerous biological processes including gene expression. The mammalian genome encodes nine protein arginine methyltransferases (Prmts) that catalyze monomethylation, asymmetric dimethylation, and symmetric dimethylation on arginine residues. Protein arginine methyltransferase 7 (Prmt7) is categorized as a type II and type III enzyme that produces symmetric dimethylated arginine and monomethylated arginine, respectively. However, the biological role of Prmt7 is not well characterized. We previously showed that Prmt5, a type II Prmt that associates with Brg1-based SWI/SNF chromatin remodeling complex, is required for adipocyte differentiation. Since Prmt7 also associates with Brg1-based SWI/SNF complex and modifies core histones, we hypothesized that Prmt7 might play a role in transcriptional regulation of adipogenesis. In the present study, we determined that the expression of Prmt7 did not change throughout adipogenic differentiation of C3H10T1/2 mesenchymal cells. Knockdown or over-expression of Prmt7 had no effect on lipid accumulation or adipogenic gene expression in differentiating C3H10T1/2 cells or in C/EBPα-reprogrammed NIH3T3 fibroblasts. Based on these results, we conclude that Prmt7, unlike Prmt5, is dispensable for adipogenic differentiation in tissue culture models. F1000Research 2013-12-18 /pmc/articles/PMC3962006/ /pubmed/24715966 http://dx.doi.org/10.12688/f1000research.2-279.v1 Text en Copyright: © 2013 Hu YJ et al. http://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. http://creativecommons.org/publicdomain/zero/1.0/ Data associated with the article are available under the terms of the Creative Commons Zero "No rights reserved" data waiver (CC0 1.0 Public domain dedication).
spellingShingle Research Article
Hu, Yu-Jie
Sif, Saïd
Imbalzano, Anthony N.
Prmt7 is dispensable in tissue culture models for adipogenic differentiation
title Prmt7 is dispensable in tissue culture models for adipogenic differentiation
title_full Prmt7 is dispensable in tissue culture models for adipogenic differentiation
title_fullStr Prmt7 is dispensable in tissue culture models for adipogenic differentiation
title_full_unstemmed Prmt7 is dispensable in tissue culture models for adipogenic differentiation
title_short Prmt7 is dispensable in tissue culture models for adipogenic differentiation
title_sort prmt7 is dispensable in tissue culture models for adipogenic differentiation
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3962006/
https://www.ncbi.nlm.nih.gov/pubmed/24715966
http://dx.doi.org/10.12688/f1000research.2-279.v1
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