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Escherichia coli redox mutants as microbial cell factories for the synthesis of reduced biochemicals
Bioprocesses conducted under conditions with restricted O(2) supply are increasingly exploited for the synthesis of reduced biochemicals using different biocatalysts. The model facultative aerobe Escherichia coli, the microbial cell factory par excellence, has elaborate sensing and signal transducti...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Research Network of Computational and Structural Biotechnology (RNCSB) Organization
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3962086/ https://www.ncbi.nlm.nih.gov/pubmed/24688679 http://dx.doi.org/10.5936/csbj.201210019 |
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author | Ruiz, Jimena A. de Almeida, Alejandra Godoy, Manuel S. Mezzina, Mariela P. Bidart, Gonzalo N. Méndez, Beatriz S. Pettinari, M. Julia Nikel, Pablo I. |
author_facet | Ruiz, Jimena A. de Almeida, Alejandra Godoy, Manuel S. Mezzina, Mariela P. Bidart, Gonzalo N. Méndez, Beatriz S. Pettinari, M. Julia Nikel, Pablo I. |
author_sort | Ruiz, Jimena A. |
collection | PubMed |
description | Bioprocesses conducted under conditions with restricted O(2) supply are increasingly exploited for the synthesis of reduced biochemicals using different biocatalysts. The model facultative aerobe Escherichia coli, the microbial cell factory par excellence, has elaborate sensing and signal transduction mechanisms that respond to the availability of electron acceptors and alternative carbon sources in the surrounding environment. In particular, the ArcBA and CreBC two-component signal transduction systems are largely responsible for the metabolic regulation of redox control in response to O(2) availability and carbon source utilization, respectively. Significant advances in the understanding of the biochemical, genetic, and physiological duties of these regulatory systems have been achieved in recent years. This situation allowed to rationally-design novel engineering approaches that ensure optimal carbon and energy flows within central metabolism, as well as to manipulate redox homeostasis, in order to optimize the production of industrially-relevant metabolites. In particular, metabolic flux analysis provided new clues to understand the metabolic regulation mediated by the ArcBA and CreBC systems. Genetic manipulation of these regulators proved useful for designing microbial cells factories tailored for the synthesis of reduced biochemicals with added value, such as poly(3-hydroxybutyrate), under conditions with restricted O(2) supply. This network-wide strategy is in contrast with traditional metabolic engineering approaches, that entail direct modification of the pathway(s) at stake, and opens new avenues for the targeted modulation of central catabolic pathways at the transcriptional level. |
format | Online Article Text |
id | pubmed-3962086 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Research Network of Computational and Structural Biotechnology (RNCSB) Organization |
record_format | MEDLINE/PubMed |
spelling | pubmed-39620862014-03-31 Escherichia coli redox mutants as microbial cell factories for the synthesis of reduced biochemicals Ruiz, Jimena A. de Almeida, Alejandra Godoy, Manuel S. Mezzina, Mariela P. Bidart, Gonzalo N. Méndez, Beatriz S. Pettinari, M. Julia Nikel, Pablo I. Comput Struct Biotechnol J Mini Review Bioprocesses conducted under conditions with restricted O(2) supply are increasingly exploited for the synthesis of reduced biochemicals using different biocatalysts. The model facultative aerobe Escherichia coli, the microbial cell factory par excellence, has elaborate sensing and signal transduction mechanisms that respond to the availability of electron acceptors and alternative carbon sources in the surrounding environment. In particular, the ArcBA and CreBC two-component signal transduction systems are largely responsible for the metabolic regulation of redox control in response to O(2) availability and carbon source utilization, respectively. Significant advances in the understanding of the biochemical, genetic, and physiological duties of these regulatory systems have been achieved in recent years. This situation allowed to rationally-design novel engineering approaches that ensure optimal carbon and energy flows within central metabolism, as well as to manipulate redox homeostasis, in order to optimize the production of industrially-relevant metabolites. In particular, metabolic flux analysis provided new clues to understand the metabolic regulation mediated by the ArcBA and CreBC systems. Genetic manipulation of these regulators proved useful for designing microbial cells factories tailored for the synthesis of reduced biochemicals with added value, such as poly(3-hydroxybutyrate), under conditions with restricted O(2) supply. This network-wide strategy is in contrast with traditional metabolic engineering approaches, that entail direct modification of the pathway(s) at stake, and opens new avenues for the targeted modulation of central catabolic pathways at the transcriptional level. Research Network of Computational and Structural Biotechnology (RNCSB) Organization 2013-01-18 /pmc/articles/PMC3962086/ /pubmed/24688679 http://dx.doi.org/10.5936/csbj.201210019 Text en © Ruiz et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly cited. |
spellingShingle | Mini Review Ruiz, Jimena A. de Almeida, Alejandra Godoy, Manuel S. Mezzina, Mariela P. Bidart, Gonzalo N. Méndez, Beatriz S. Pettinari, M. Julia Nikel, Pablo I. Escherichia coli redox mutants as microbial cell factories for the synthesis of reduced biochemicals |
title |
Escherichia coli redox mutants as microbial cell factories for the synthesis of reduced biochemicals |
title_full |
Escherichia coli redox mutants as microbial cell factories for the synthesis of reduced biochemicals |
title_fullStr |
Escherichia coli redox mutants as microbial cell factories for the synthesis of reduced biochemicals |
title_full_unstemmed |
Escherichia coli redox mutants as microbial cell factories for the synthesis of reduced biochemicals |
title_short |
Escherichia coli redox mutants as microbial cell factories for the synthesis of reduced biochemicals |
title_sort | escherichia coli redox mutants as microbial cell factories for the synthesis of reduced biochemicals |
topic | Mini Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3962086/ https://www.ncbi.nlm.nih.gov/pubmed/24688679 http://dx.doi.org/10.5936/csbj.201210019 |
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