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A structural overview of GH61 proteins – fungal cellulose degrading polysaccharide monooxygenases

Recent years have witnessed a spurt of activities in the elucidation of the molecular function of a class of proteins with great potential in biomass degradation. GH61 proteins are of fungal origin and were originally classified in family 61 of the glycoside hydrolases. From the beginning they were...

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Autores principales: Leggio, Leila Lo, Welner, Ditte, De Maria, Leonardo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Research Network of Computational and Structural Biotechnology (RNCSB) Organization 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3962118/
https://www.ncbi.nlm.nih.gov/pubmed/24688660
http://dx.doi.org/10.5936/csbj.201209019
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author Leggio, Leila Lo
Welner, Ditte
De Maria, Leonardo
author_facet Leggio, Leila Lo
Welner, Ditte
De Maria, Leonardo
author_sort Leggio, Leila Lo
collection PubMed
description Recent years have witnessed a spurt of activities in the elucidation of the molecular function of a class of proteins with great potential in biomass degradation. GH61 proteins are of fungal origin and were originally classified in family 61 of the glycoside hydrolases. From the beginning they were strongly suspected to be involved in cellulose degradation because of their expression profiles, despite very low detectable endoglucanase activities. A major breakthrough came from structure determination of the first members, establishing the presence of a divalent metal binding site and a similarity to bacterial proteins involved in chitin degradation. A second breakthrough came from the identification of cellulase boosting activity dependent on the integrity of the metal binding site. Finally very recently GH61 proteins were demonstrated to oxidatively cleave crystalline cellulose in a Cu and reductant dependant manner. This mini-review in particular focuses on the contribution that structure elucidation has made in the understanding of GH61 molecular function and reviews the currently known structures and the challenges remaining ahead for exploiting this new class of enzymes to the full.
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spelling pubmed-39621182014-03-31 A structural overview of GH61 proteins – fungal cellulose degrading polysaccharide monooxygenases Leggio, Leila Lo Welner, Ditte De Maria, Leonardo Comput Struct Biotechnol J Mini Review Recent years have witnessed a spurt of activities in the elucidation of the molecular function of a class of proteins with great potential in biomass degradation. GH61 proteins are of fungal origin and were originally classified in family 61 of the glycoside hydrolases. From the beginning they were strongly suspected to be involved in cellulose degradation because of their expression profiles, despite very low detectable endoglucanase activities. A major breakthrough came from structure determination of the first members, establishing the presence of a divalent metal binding site and a similarity to bacterial proteins involved in chitin degradation. A second breakthrough came from the identification of cellulase boosting activity dependent on the integrity of the metal binding site. Finally very recently GH61 proteins were demonstrated to oxidatively cleave crystalline cellulose in a Cu and reductant dependant manner. This mini-review in particular focuses on the contribution that structure elucidation has made in the understanding of GH61 molecular function and reviews the currently known structures and the challenges remaining ahead for exploiting this new class of enzymes to the full. Research Network of Computational and Structural Biotechnology (RNCSB) Organization 2012-11-30 /pmc/articles/PMC3962118/ /pubmed/24688660 http://dx.doi.org/10.5936/csbj.201209019 Text en © Lo Leggio et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly cited.
spellingShingle Mini Review
Leggio, Leila Lo
Welner, Ditte
De Maria, Leonardo
A structural overview of GH61 proteins – fungal cellulose degrading polysaccharide monooxygenases
title A structural overview of GH61 proteins – fungal cellulose degrading polysaccharide monooxygenases
title_full A structural overview of GH61 proteins – fungal cellulose degrading polysaccharide monooxygenases
title_fullStr A structural overview of GH61 proteins – fungal cellulose degrading polysaccharide monooxygenases
title_full_unstemmed A structural overview of GH61 proteins – fungal cellulose degrading polysaccharide monooxygenases
title_short A structural overview of GH61 proteins – fungal cellulose degrading polysaccharide monooxygenases
title_sort structural overview of gh61 proteins – fungal cellulose degrading polysaccharide monooxygenases
topic Mini Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3962118/
https://www.ncbi.nlm.nih.gov/pubmed/24688660
http://dx.doi.org/10.5936/csbj.201209019
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