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Cancer cell-oriented migration of mesenchymal stem cells engineered with an anticancer gene (PTEN): an imaging demonstration

BACKGROUND: Mesenchymal stem cells (MSCs) have been considered to hold great potential as ideal carriers for the delivery of anticancer agents since the discovery of their tumor tropism. This study was performed to demonstrate the effects of phosphatase and tensin homolog (PTEN) engineering on MSCs’...

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Autores principales: Yang, Zhuo-Shun, Tang, Xiang-Jun, Guo, Xing-Rong, Zou, Dan-Dan, Sun, Xu-Yong, Feng, Jing-Bo, Luo, Jie, Dai, Long-Jun, Warnock, Garth L
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3962313/
https://www.ncbi.nlm.nih.gov/pubmed/24669193
http://dx.doi.org/10.2147/OTT.S59227
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author Yang, Zhuo-Shun
Tang, Xiang-Jun
Guo, Xing-Rong
Zou, Dan-Dan
Sun, Xu-Yong
Feng, Jing-Bo
Luo, Jie
Dai, Long-Jun
Warnock, Garth L
author_facet Yang, Zhuo-Shun
Tang, Xiang-Jun
Guo, Xing-Rong
Zou, Dan-Dan
Sun, Xu-Yong
Feng, Jing-Bo
Luo, Jie
Dai, Long-Jun
Warnock, Garth L
author_sort Yang, Zhuo-Shun
collection PubMed
description BACKGROUND: Mesenchymal stem cells (MSCs) have been considered to hold great potential as ideal carriers for the delivery of anticancer agents since the discovery of their tumor tropism. This study was performed to demonstrate the effects of phosphatase and tensin homolog (PTEN) engineering on MSCs’ capacity for cancer cell-oriented migration. METHODS: MSCs were engineered with a PTEN-bearing plasmid and the expression was confirmed with Western blotting. A human glioma cell line (DBTRG) was used as the target cell; DBTRG cell-oriented migration of MSCs was monitored with a micro speed photographic system. RESULTS: The expression of transfected PTEN in MSCs was identified by immunoblotting analysis and confirmed with cell viability assessment of target cells. The DBTRG cell-oriented migration of PTEN-engineered MSCs was demonstrated by a real-time dynamic monitoring system, and a phagocytosis-like action of MSCs was also observed. CONCLUSION: MSCs maintained their capacity for cancer cell-directed migration after they were engineered with anticancer genes. This study provides the first direct evidence of MSCs’ tropism post-anticancer gene engineering.
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spelling pubmed-39623132014-03-25 Cancer cell-oriented migration of mesenchymal stem cells engineered with an anticancer gene (PTEN): an imaging demonstration Yang, Zhuo-Shun Tang, Xiang-Jun Guo, Xing-Rong Zou, Dan-Dan Sun, Xu-Yong Feng, Jing-Bo Luo, Jie Dai, Long-Jun Warnock, Garth L Onco Targets Ther Original Research BACKGROUND: Mesenchymal stem cells (MSCs) have been considered to hold great potential as ideal carriers for the delivery of anticancer agents since the discovery of their tumor tropism. This study was performed to demonstrate the effects of phosphatase and tensin homolog (PTEN) engineering on MSCs’ capacity for cancer cell-oriented migration. METHODS: MSCs were engineered with a PTEN-bearing plasmid and the expression was confirmed with Western blotting. A human glioma cell line (DBTRG) was used as the target cell; DBTRG cell-oriented migration of MSCs was monitored with a micro speed photographic system. RESULTS: The expression of transfected PTEN in MSCs was identified by immunoblotting analysis and confirmed with cell viability assessment of target cells. The DBTRG cell-oriented migration of PTEN-engineered MSCs was demonstrated by a real-time dynamic monitoring system, and a phagocytosis-like action of MSCs was also observed. CONCLUSION: MSCs maintained their capacity for cancer cell-directed migration after they were engineered with anticancer genes. This study provides the first direct evidence of MSCs’ tropism post-anticancer gene engineering. Dove Medical Press 2014-03-17 /pmc/articles/PMC3962313/ /pubmed/24669193 http://dx.doi.org/10.2147/OTT.S59227 Text en © 2014 Yang et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.
spellingShingle Original Research
Yang, Zhuo-Shun
Tang, Xiang-Jun
Guo, Xing-Rong
Zou, Dan-Dan
Sun, Xu-Yong
Feng, Jing-Bo
Luo, Jie
Dai, Long-Jun
Warnock, Garth L
Cancer cell-oriented migration of mesenchymal stem cells engineered with an anticancer gene (PTEN): an imaging demonstration
title Cancer cell-oriented migration of mesenchymal stem cells engineered with an anticancer gene (PTEN): an imaging demonstration
title_full Cancer cell-oriented migration of mesenchymal stem cells engineered with an anticancer gene (PTEN): an imaging demonstration
title_fullStr Cancer cell-oriented migration of mesenchymal stem cells engineered with an anticancer gene (PTEN): an imaging demonstration
title_full_unstemmed Cancer cell-oriented migration of mesenchymal stem cells engineered with an anticancer gene (PTEN): an imaging demonstration
title_short Cancer cell-oriented migration of mesenchymal stem cells engineered with an anticancer gene (PTEN): an imaging demonstration
title_sort cancer cell-oriented migration of mesenchymal stem cells engineered with an anticancer gene (pten): an imaging demonstration
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3962313/
https://www.ncbi.nlm.nih.gov/pubmed/24669193
http://dx.doi.org/10.2147/OTT.S59227
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