Cargando…
Monolayer spontaneous curvature of raft-forming membrane lipids
Monolayer spontaneous curvatures for cholesterol, DOPE, POPE, DOPC, DPPC, DSPC, POPC, SOPC, and egg sphingomyelin were obtained using small-angle X-ray scattering (SAXS) on inverted hexagonal phases (H(II)). Spontaneous curvatures of bilayer forming lipids were estimated by adding controlled amounts...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3963256/ https://www.ncbi.nlm.nih.gov/pubmed/24672578 http://dx.doi.org/10.1039/C3SM51829A |
_version_ | 1782308487593721856 |
---|---|
author | Kollmitzer, Benjamin Heftberger, Peter Rappolt, Michael Pabst, Georg |
author_facet | Kollmitzer, Benjamin Heftberger, Peter Rappolt, Michael Pabst, Georg |
author_sort | Kollmitzer, Benjamin |
collection | PubMed |
description | Monolayer spontaneous curvatures for cholesterol, DOPE, POPE, DOPC, DPPC, DSPC, POPC, SOPC, and egg sphingomyelin were obtained using small-angle X-ray scattering (SAXS) on inverted hexagonal phases (H(II)). Spontaneous curvatures of bilayer forming lipids were estimated by adding controlled amounts to a H(II) forming template following previously established protocols. Spontaneous curvatures of both phosphatidylethanolamines and cholesterol were found to be at least a factor of two more negative than those of phosphatidylcholines, whose J(0) values are closer to zero. Interestingly, a significant positive J(0) value was retrieved for DPPC. We further determined the temperature dependence of the spontaneous curvatures J(0)(T) in the range from 15 to 55 °C, resulting in a quite narrow distribution of −1 to −3 × 10(−3) (nm °C)(−1) for most investigated lipids. The data allowed us to estimate the monolayer spontaneous curvatures of ternary lipid mixtures showing liquid ordered/liquid disordered phase coexistence. We report spontaneous curvature phase diagrams for DSPC/DOPC/Chol, DPPC/DOPC/Chol and SM/POPC/Chol and discuss effects on protein insertion and line tension. |
format | Online Article Text |
id | pubmed-3963256 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
record_format | MEDLINE/PubMed |
spelling | pubmed-39632562014-03-24 Monolayer spontaneous curvature of raft-forming membrane lipids Kollmitzer, Benjamin Heftberger, Peter Rappolt, Michael Pabst, Georg Soft Matter Article Monolayer spontaneous curvatures for cholesterol, DOPE, POPE, DOPC, DPPC, DSPC, POPC, SOPC, and egg sphingomyelin were obtained using small-angle X-ray scattering (SAXS) on inverted hexagonal phases (H(II)). Spontaneous curvatures of bilayer forming lipids were estimated by adding controlled amounts to a H(II) forming template following previously established protocols. Spontaneous curvatures of both phosphatidylethanolamines and cholesterol were found to be at least a factor of two more negative than those of phosphatidylcholines, whose J(0) values are closer to zero. Interestingly, a significant positive J(0) value was retrieved for DPPC. We further determined the temperature dependence of the spontaneous curvatures J(0)(T) in the range from 15 to 55 °C, resulting in a quite narrow distribution of −1 to −3 × 10(−3) (nm °C)(−1) for most investigated lipids. The data allowed us to estimate the monolayer spontaneous curvatures of ternary lipid mixtures showing liquid ordered/liquid disordered phase coexistence. We report spontaneous curvature phase diagrams for DSPC/DOPC/Chol, DPPC/DOPC/Chol and SM/POPC/Chol and discuss effects on protein insertion and line tension. 2013-12-07 /pmc/articles/PMC3963256/ /pubmed/24672578 http://dx.doi.org/10.1039/C3SM51829A Text en © The Royal Society of Chemistry 2013 http://creativecommons.org/licenses/by-nc/3.0/ This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. |
spellingShingle | Article Kollmitzer, Benjamin Heftberger, Peter Rappolt, Michael Pabst, Georg Monolayer spontaneous curvature of raft-forming membrane lipids |
title | Monolayer spontaneous curvature of raft-forming membrane lipids |
title_full | Monolayer spontaneous curvature of raft-forming membrane lipids |
title_fullStr | Monolayer spontaneous curvature of raft-forming membrane lipids |
title_full_unstemmed | Monolayer spontaneous curvature of raft-forming membrane lipids |
title_short | Monolayer spontaneous curvature of raft-forming membrane lipids |
title_sort | monolayer spontaneous curvature of raft-forming membrane lipids |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3963256/ https://www.ncbi.nlm.nih.gov/pubmed/24672578 http://dx.doi.org/10.1039/C3SM51829A |
work_keys_str_mv | AT kollmitzerbenjamin monolayerspontaneouscurvatureofraftformingmembranelipids AT heftbergerpeter monolayerspontaneouscurvatureofraftformingmembranelipids AT rappoltmichael monolayerspontaneouscurvatureofraftformingmembranelipids AT pabstgeorg monolayerspontaneouscurvatureofraftformingmembranelipids |