Cargando…

Monolayer spontaneous curvature of raft-forming membrane lipids

Monolayer spontaneous curvatures for cholesterol, DOPE, POPE, DOPC, DPPC, DSPC, POPC, SOPC, and egg sphingomyelin were obtained using small-angle X-ray scattering (SAXS) on inverted hexagonal phases (H(II)). Spontaneous curvatures of bilayer forming lipids were estimated by adding controlled amounts...

Descripción completa

Detalles Bibliográficos
Autores principales: Kollmitzer, Benjamin, Heftberger, Peter, Rappolt, Michael, Pabst, Georg
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3963256/
https://www.ncbi.nlm.nih.gov/pubmed/24672578
http://dx.doi.org/10.1039/C3SM51829A
_version_ 1782308487593721856
author Kollmitzer, Benjamin
Heftberger, Peter
Rappolt, Michael
Pabst, Georg
author_facet Kollmitzer, Benjamin
Heftberger, Peter
Rappolt, Michael
Pabst, Georg
author_sort Kollmitzer, Benjamin
collection PubMed
description Monolayer spontaneous curvatures for cholesterol, DOPE, POPE, DOPC, DPPC, DSPC, POPC, SOPC, and egg sphingomyelin were obtained using small-angle X-ray scattering (SAXS) on inverted hexagonal phases (H(II)). Spontaneous curvatures of bilayer forming lipids were estimated by adding controlled amounts to a H(II) forming template following previously established protocols. Spontaneous curvatures of both phosphatidylethanolamines and cholesterol were found to be at least a factor of two more negative than those of phosphatidylcholines, whose J(0) values are closer to zero. Interestingly, a significant positive J(0) value was retrieved for DPPC. We further determined the temperature dependence of the spontaneous curvatures J(0)(T) in the range from 15 to 55 °C, resulting in a quite narrow distribution of −1 to −3 × 10(−3) (nm °C)(−1) for most investigated lipids. The data allowed us to estimate the monolayer spontaneous curvatures of ternary lipid mixtures showing liquid ordered/liquid disordered phase coexistence. We report spontaneous curvature phase diagrams for DSPC/DOPC/Chol, DPPC/DOPC/Chol and SM/POPC/Chol and discuss effects on protein insertion and line tension.
format Online
Article
Text
id pubmed-3963256
institution National Center for Biotechnology Information
language English
publishDate 2013
record_format MEDLINE/PubMed
spelling pubmed-39632562014-03-24 Monolayer spontaneous curvature of raft-forming membrane lipids Kollmitzer, Benjamin Heftberger, Peter Rappolt, Michael Pabst, Georg Soft Matter Article Monolayer spontaneous curvatures for cholesterol, DOPE, POPE, DOPC, DPPC, DSPC, POPC, SOPC, and egg sphingomyelin were obtained using small-angle X-ray scattering (SAXS) on inverted hexagonal phases (H(II)). Spontaneous curvatures of bilayer forming lipids were estimated by adding controlled amounts to a H(II) forming template following previously established protocols. Spontaneous curvatures of both phosphatidylethanolamines and cholesterol were found to be at least a factor of two more negative than those of phosphatidylcholines, whose J(0) values are closer to zero. Interestingly, a significant positive J(0) value was retrieved for DPPC. We further determined the temperature dependence of the spontaneous curvatures J(0)(T) in the range from 15 to 55 °C, resulting in a quite narrow distribution of −1 to −3 × 10(−3) (nm °C)(−1) for most investigated lipids. The data allowed us to estimate the monolayer spontaneous curvatures of ternary lipid mixtures showing liquid ordered/liquid disordered phase coexistence. We report spontaneous curvature phase diagrams for DSPC/DOPC/Chol, DPPC/DOPC/Chol and SM/POPC/Chol and discuss effects on protein insertion and line tension. 2013-12-07 /pmc/articles/PMC3963256/ /pubmed/24672578 http://dx.doi.org/10.1039/C3SM51829A Text en © The Royal Society of Chemistry 2013 http://creativecommons.org/licenses/by-nc/3.0/ This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence.
spellingShingle Article
Kollmitzer, Benjamin
Heftberger, Peter
Rappolt, Michael
Pabst, Georg
Monolayer spontaneous curvature of raft-forming membrane lipids
title Monolayer spontaneous curvature of raft-forming membrane lipids
title_full Monolayer spontaneous curvature of raft-forming membrane lipids
title_fullStr Monolayer spontaneous curvature of raft-forming membrane lipids
title_full_unstemmed Monolayer spontaneous curvature of raft-forming membrane lipids
title_short Monolayer spontaneous curvature of raft-forming membrane lipids
title_sort monolayer spontaneous curvature of raft-forming membrane lipids
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3963256/
https://www.ncbi.nlm.nih.gov/pubmed/24672578
http://dx.doi.org/10.1039/C3SM51829A
work_keys_str_mv AT kollmitzerbenjamin monolayerspontaneouscurvatureofraftformingmembranelipids
AT heftbergerpeter monolayerspontaneouscurvatureofraftformingmembranelipids
AT rappoltmichael monolayerspontaneouscurvatureofraftformingmembranelipids
AT pabstgeorg monolayerspontaneouscurvatureofraftformingmembranelipids