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Purified Culture Systems for Bovine Oviductal Stromal Cells
Isolated stromal cells from the ampullary and isthmic parts of bovine oviductal tissues were cultured in monolayer and spheroid (cell aggregate) systems. Prostaglandin F2α (PGF) plays a crucial role in oviductal contraction and is produced by oviductal epithelial cells in cattle. Since stromal cells...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Society for Reproduction and Development
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3963300/ https://www.ncbi.nlm.nih.gov/pubmed/24096613 http://dx.doi.org/10.1262/jrd.2013-061 |
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author | YAMAMOTO, Yuki KOBAYASHI, Yoshihiko OKUDA, Kiyoshi |
author_facet | YAMAMOTO, Yuki KOBAYASHI, Yoshihiko OKUDA, Kiyoshi |
author_sort | YAMAMOTO, Yuki |
collection | PubMed |
description | Isolated stromal cells from the ampullary and isthmic parts of bovine oviductal tissues were cultured in monolayer and spheroid (cell aggregate) systems. Prostaglandin F2α (PGF) plays a crucial role in oviductal contraction and is produced by oviductal epithelial cells in cattle. Since stromal cells of many organs produce PGF, PGF production by bovine oviductal stromal cells was investigated. After PGF synthesis was confirmed, the utility of isolation and culture methods for oviductal stromal cells was evaluated by PGF production in the present study. The homogeneity of the cells was > 99%. PGF production of the cells was increased by tumor necrosis factor-α. The stromal cells aggregated and formed a spheroid by the treatments with several reagents. PGF production was higher in the spheroid culture than in the monolayer culture. The isolation and culture methods described here will facilitate studies of the physiological function of bovine oviductal stromal cells. |
format | Online Article Text |
id | pubmed-3963300 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | The Society for Reproduction and Development |
record_format | MEDLINE/PubMed |
spelling | pubmed-39633002014-03-24 Purified Culture Systems for Bovine Oviductal Stromal Cells YAMAMOTO, Yuki KOBAYASHI, Yoshihiko OKUDA, Kiyoshi J Reprod Dev Technology Report Isolated stromal cells from the ampullary and isthmic parts of bovine oviductal tissues were cultured in monolayer and spheroid (cell aggregate) systems. Prostaglandin F2α (PGF) plays a crucial role in oviductal contraction and is produced by oviductal epithelial cells in cattle. Since stromal cells of many organs produce PGF, PGF production by bovine oviductal stromal cells was investigated. After PGF synthesis was confirmed, the utility of isolation and culture methods for oviductal stromal cells was evaluated by PGF production in the present study. The homogeneity of the cells was > 99%. PGF production of the cells was increased by tumor necrosis factor-α. The stromal cells aggregated and formed a spheroid by the treatments with several reagents. PGF production was higher in the spheroid culture than in the monolayer culture. The isolation and culture methods described here will facilitate studies of the physiological function of bovine oviductal stromal cells. The Society for Reproduction and Development 2013-10-05 2014-02 /pmc/articles/PMC3963300/ /pubmed/24096613 http://dx.doi.org/10.1262/jrd.2013-061 Text en ©2014 Society for Reproduction and Development http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. |
spellingShingle | Technology Report YAMAMOTO, Yuki KOBAYASHI, Yoshihiko OKUDA, Kiyoshi Purified Culture Systems for Bovine Oviductal Stromal Cells |
title | Purified Culture Systems for Bovine Oviductal Stromal Cells |
title_full | Purified Culture Systems for Bovine Oviductal Stromal Cells |
title_fullStr | Purified Culture Systems for Bovine Oviductal Stromal Cells |
title_full_unstemmed | Purified Culture Systems for Bovine Oviductal Stromal Cells |
title_short | Purified Culture Systems for Bovine Oviductal Stromal Cells |
title_sort | purified culture systems for bovine oviductal stromal cells |
topic | Technology Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3963300/ https://www.ncbi.nlm.nih.gov/pubmed/24096613 http://dx.doi.org/10.1262/jrd.2013-061 |
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