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Insights into controlling role of substitution mutation, E315G on thermostability of a lipase cloned from metagenome of hot spring soil
Rational mutagenesis was performed (at the vicinity of the active site residues D317 and H358 of a mature polypeptide) to investigate the role of amino acids in the thermostability/activity of a lipase enzyme. The single variant enzyme created with E315G (lip M2) mutation near one of the active site...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Springer Berlin Heidelberg
2013
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3964248/ https://www.ncbi.nlm.nih.gov/pubmed/28324449 http://dx.doi.org/10.1007/s13205-013-0142-4 |