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A microscale anisotropic biaxial cell stretching device for applications in mechanobiology
A multi-layered polydimethylsiloxane microfluidic device with an integrated suspended membrane has been fabricated that allows dynamic and multi-axial mechanical deformation and simultaneous live-cell microscopy imaging. The transparent membrane’s strain field can be controlled independently along t...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Netherlands
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3964308/ https://www.ncbi.nlm.nih.gov/pubmed/24129957 http://dx.doi.org/10.1007/s10529-013-1381-5 |
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author | Tremblay, Dominique Chagnon-Lessard, Sophie Mirzaei, Maryam Pelling, Andrew E. Godin, Michel |
author_facet | Tremblay, Dominique Chagnon-Lessard, Sophie Mirzaei, Maryam Pelling, Andrew E. Godin, Michel |
author_sort | Tremblay, Dominique |
collection | PubMed |
description | A multi-layered polydimethylsiloxane microfluidic device with an integrated suspended membrane has been fabricated that allows dynamic and multi-axial mechanical deformation and simultaneous live-cell microscopy imaging. The transparent membrane’s strain field can be controlled independently along two orthogonal directions. Human foreskin fibroblasts were immobilized on the membrane’s surface and stretched along two orthogonal directions sequentially while performing live-cell imaging. Cyclic deformation of the cells induced a reversible reorientation perpendicular to the direction of the applied strain. Cells remained viable in the microdevice for several days. As opposed to existing microfluidic or macroscale stretching devices, this device can impose changing, anisotropic and time-varying strain fields in order to more closely mimic the complexities of strains occurring in vivo. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s10529-013-1381-5) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-3964308 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Springer Netherlands |
record_format | MEDLINE/PubMed |
spelling | pubmed-39643082014-03-25 A microscale anisotropic biaxial cell stretching device for applications in mechanobiology Tremblay, Dominique Chagnon-Lessard, Sophie Mirzaei, Maryam Pelling, Andrew E. Godin, Michel Biotechnol Lett Original Research Paper A multi-layered polydimethylsiloxane microfluidic device with an integrated suspended membrane has been fabricated that allows dynamic and multi-axial mechanical deformation and simultaneous live-cell microscopy imaging. The transparent membrane’s strain field can be controlled independently along two orthogonal directions. Human foreskin fibroblasts were immobilized on the membrane’s surface and stretched along two orthogonal directions sequentially while performing live-cell imaging. Cyclic deformation of the cells induced a reversible reorientation perpendicular to the direction of the applied strain. Cells remained viable in the microdevice for several days. As opposed to existing microfluidic or macroscale stretching devices, this device can impose changing, anisotropic and time-varying strain fields in order to more closely mimic the complexities of strains occurring in vivo. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s10529-013-1381-5) contains supplementary material, which is available to authorized users. Springer Netherlands 2013-10-16 2014 /pmc/articles/PMC3964308/ /pubmed/24129957 http://dx.doi.org/10.1007/s10529-013-1381-5 Text en © The Author(s) 2013 https://creativecommons.org/licenses/by/2.0/ Open AccessThis article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited. |
spellingShingle | Original Research Paper Tremblay, Dominique Chagnon-Lessard, Sophie Mirzaei, Maryam Pelling, Andrew E. Godin, Michel A microscale anisotropic biaxial cell stretching device for applications in mechanobiology |
title | A microscale anisotropic biaxial cell stretching device for applications in mechanobiology |
title_full | A microscale anisotropic biaxial cell stretching device for applications in mechanobiology |
title_fullStr | A microscale anisotropic biaxial cell stretching device for applications in mechanobiology |
title_full_unstemmed | A microscale anisotropic biaxial cell stretching device for applications in mechanobiology |
title_short | A microscale anisotropic biaxial cell stretching device for applications in mechanobiology |
title_sort | microscale anisotropic biaxial cell stretching device for applications in mechanobiology |
topic | Original Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3964308/ https://www.ncbi.nlm.nih.gov/pubmed/24129957 http://dx.doi.org/10.1007/s10529-013-1381-5 |
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