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Hydrogel microfluidics for the patterning of pluripotent stem cells
Biomolecular signaling is of utmost importance in governing many biological processes such as the patterning of the developing embryo where biomolecules regulate key cell-fate decisions. In vivo, these factors are presented in a spatiotemporally tightly controlled fashion. Although state-of-the-art...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3964519/ https://www.ncbi.nlm.nih.gov/pubmed/24662945 http://dx.doi.org/10.1038/srep04462 |
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author | Cosson, S. Lutolf, M. P. |
author_facet | Cosson, S. Lutolf, M. P. |
author_sort | Cosson, S. |
collection | PubMed |
description | Biomolecular signaling is of utmost importance in governing many biological processes such as the patterning of the developing embryo where biomolecules regulate key cell-fate decisions. In vivo, these factors are presented in a spatiotemporally tightly controlled fashion. Although state-of-the-art microfluidic technologies allow precise biomolecule delivery in time and space, long-term (stem) cell culture at the micro-scale is often far from ideal due to medium evaporation, limited space for cell growth or shear stress. To overcome these challenges, we here introduce a concept based on hydrogel microfluidics for decoupling conventional, macro-scale cell culture from precise biomolecule delivery through a gel layer. We demonstrate the spatiotemporally controlled neuronal commitment of mouse embryonic stem cells via delivery of retinoic acid gradients. This technique should be useful for testing the effect of dose and timing of biomolecules, singly or in combination, on stem cell fate. |
format | Online Article Text |
id | pubmed-3964519 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-39645192014-03-26 Hydrogel microfluidics for the patterning of pluripotent stem cells Cosson, S. Lutolf, M. P. Sci Rep Article Biomolecular signaling is of utmost importance in governing many biological processes such as the patterning of the developing embryo where biomolecules regulate key cell-fate decisions. In vivo, these factors are presented in a spatiotemporally tightly controlled fashion. Although state-of-the-art microfluidic technologies allow precise biomolecule delivery in time and space, long-term (stem) cell culture at the micro-scale is often far from ideal due to medium evaporation, limited space for cell growth or shear stress. To overcome these challenges, we here introduce a concept based on hydrogel microfluidics for decoupling conventional, macro-scale cell culture from precise biomolecule delivery through a gel layer. We demonstrate the spatiotemporally controlled neuronal commitment of mouse embryonic stem cells via delivery of retinoic acid gradients. This technique should be useful for testing the effect of dose and timing of biomolecules, singly or in combination, on stem cell fate. Nature Publishing Group 2014-03-25 /pmc/articles/PMC3964519/ /pubmed/24662945 http://dx.doi.org/10.1038/srep04462 Text en Copyright © 2014, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-nd/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported license. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/ |
spellingShingle | Article Cosson, S. Lutolf, M. P. Hydrogel microfluidics for the patterning of pluripotent stem cells |
title | Hydrogel microfluidics for the patterning of pluripotent stem cells |
title_full | Hydrogel microfluidics for the patterning of pluripotent stem cells |
title_fullStr | Hydrogel microfluidics for the patterning of pluripotent stem cells |
title_full_unstemmed | Hydrogel microfluidics for the patterning of pluripotent stem cells |
title_short | Hydrogel microfluidics for the patterning of pluripotent stem cells |
title_sort | hydrogel microfluidics for the patterning of pluripotent stem cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3964519/ https://www.ncbi.nlm.nih.gov/pubmed/24662945 http://dx.doi.org/10.1038/srep04462 |
work_keys_str_mv | AT cossons hydrogelmicrofluidicsforthepatterningofpluripotentstemcells AT lutolfmp hydrogelmicrofluidicsforthepatterningofpluripotentstemcells |