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Osteoblasts Growth Behaviour on Bio-Based Calcium Carbonate Aragonite Nanocrystal

Calcium carbonate (CaCO(3)) nanocrystals derived from cockle shells emerge to present a good concert in bone tissue engineering because of their potential to mimic the composition, structure, and properties of native bone. The aim of this study was to evaluate the biological response of CaCO(3) nano...

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Detalles Bibliográficos
Autores principales: Shafiu Kamba, Abdullahi, Zakaria, Zuki Abu Bakar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3964785/
https://www.ncbi.nlm.nih.gov/pubmed/24734228
http://dx.doi.org/10.1155/2014/215097
Descripción
Sumario:Calcium carbonate (CaCO(3)) nanocrystals derived from cockle shells emerge to present a good concert in bone tissue engineering because of their potential to mimic the composition, structure, and properties of native bone. The aim of this study was to evaluate the biological response of CaCO(3) nanocrystals on hFOB 1.19 and MC3T3 E-1 osteoblast cells in vitro. Cell viability and proliferation were assessed by MTT and BrdU assays, and LDH was measured to determine the effect of CaCO(3) nanocrystals on cell membrane integrity. Cellular morphology was examined by SEM and fluorescence microscopy. The results showed that CaCO(3) nanocrystals had no toxic effects to some extent. Cell proliferation, alkaline phosphatase activity, and protein synthesis were enhanced by the nanocrystals when compared to the control. Cellular interactions were improved, as indicated by SEM and fluorescent microscopy. The production of VEGF and TGF-1 was also affected by the CaCO(3) nanocrystals. Therefore, bio-based CaCO(3) nanocrystals were shown to stimulate osteoblast differentiation and improve the osteointegration process.