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Antifungal, Antileishmanial, and Cytotoxicity Activities of Various Extracts of Berberis vulgaris (Berberidaceae) and Its Active Principle Berberine
In this study, in vitro antidermatophytic activity against Trichophyton mentagrophytes, Trichophyton rubrum, Microsporum canis, and Microsporum gypseum was studied by disk diffusion test and assessment of minimum inhibitory concentration (MIC) using CLSI broth macrodilution method (M38-A2). Moreover...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Hindawi Publishing Corporation
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3964876/ https://www.ncbi.nlm.nih.gov/pubmed/24977052 http://dx.doi.org/10.1155/2014/602436 |
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author | Mahmoudvand, Hossein Ayatollahi Mousavi, Seyyed Amin Sepahvand, Asghar Sharififar, Fariba Ezatpour, Behrouz Gorohi, Fatemeh Saedi Dezaki, Ebrahim Jahanbakhsh, Sareh |
author_facet | Mahmoudvand, Hossein Ayatollahi Mousavi, Seyyed Amin Sepahvand, Asghar Sharififar, Fariba Ezatpour, Behrouz Gorohi, Fatemeh Saedi Dezaki, Ebrahim Jahanbakhsh, Sareh |
author_sort | Mahmoudvand, Hossein |
collection | PubMed |
description | In this study, in vitro antidermatophytic activity against Trichophyton mentagrophytes, Trichophyton rubrum, Microsporum canis, and Microsporum gypseum was studied by disk diffusion test and assessment of minimum inhibitory concentration (MIC) using CLSI broth macrodilution method (M38-A2). Moreover, antileishmanial and cytotoxicity activity of B. vulgaris and berberine against promastigotes of Leishmania major and Leishmania tropica were evaluated by colorimetric MTT assay. The findings indicated that the various extracts of B. vulgaris particularly berberine showed high potential antidermatophytic against pathogenic dermatophytes tested with MIC values varying from 0.125 to >4 mg/mL. The results revealed that B. vulgaris extracts as well as berberine were effective in inhibiting L. major and L. tropica promastigotes growth in a dose-dependent manner with IC(50) (50% inhibitory concentration) values varying from 2.1 to 26.6 μg/mL. Moreover, it could be observed that berberine as compared with B. vulgaris exhibited more cytotoxicity against murine macrophages with CC(50) (cytotoxicity concentration for 50% of cells) values varying from 27.3 to 362.6 μg/mL. Results of this investigation were the first step in the search for new antidermatophytic and antileishmanial drugs. However, further works are required to evaluate exact effect of these extracts in animal models as well as volunteer human subjects. |
format | Online Article Text |
id | pubmed-3964876 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-39648762014-06-29 Antifungal, Antileishmanial, and Cytotoxicity Activities of Various Extracts of Berberis vulgaris (Berberidaceae) and Its Active Principle Berberine Mahmoudvand, Hossein Ayatollahi Mousavi, Seyyed Amin Sepahvand, Asghar Sharififar, Fariba Ezatpour, Behrouz Gorohi, Fatemeh Saedi Dezaki, Ebrahim Jahanbakhsh, Sareh ISRN Pharmacol Research Article In this study, in vitro antidermatophytic activity against Trichophyton mentagrophytes, Trichophyton rubrum, Microsporum canis, and Microsporum gypseum was studied by disk diffusion test and assessment of minimum inhibitory concentration (MIC) using CLSI broth macrodilution method (M38-A2). Moreover, antileishmanial and cytotoxicity activity of B. vulgaris and berberine against promastigotes of Leishmania major and Leishmania tropica were evaluated by colorimetric MTT assay. The findings indicated that the various extracts of B. vulgaris particularly berberine showed high potential antidermatophytic against pathogenic dermatophytes tested with MIC values varying from 0.125 to >4 mg/mL. The results revealed that B. vulgaris extracts as well as berberine were effective in inhibiting L. major and L. tropica promastigotes growth in a dose-dependent manner with IC(50) (50% inhibitory concentration) values varying from 2.1 to 26.6 μg/mL. Moreover, it could be observed that berberine as compared with B. vulgaris exhibited more cytotoxicity against murine macrophages with CC(50) (cytotoxicity concentration for 50% of cells) values varying from 27.3 to 362.6 μg/mL. Results of this investigation were the first step in the search for new antidermatophytic and antileishmanial drugs. However, further works are required to evaluate exact effect of these extracts in animal models as well as volunteer human subjects. Hindawi Publishing Corporation 2014-03-10 /pmc/articles/PMC3964876/ /pubmed/24977052 http://dx.doi.org/10.1155/2014/602436 Text en Copyright © 2014 Hossein Mahmoudvand et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Mahmoudvand, Hossein Ayatollahi Mousavi, Seyyed Amin Sepahvand, Asghar Sharififar, Fariba Ezatpour, Behrouz Gorohi, Fatemeh Saedi Dezaki, Ebrahim Jahanbakhsh, Sareh Antifungal, Antileishmanial, and Cytotoxicity Activities of Various Extracts of Berberis vulgaris (Berberidaceae) and Its Active Principle Berberine |
title | Antifungal, Antileishmanial, and Cytotoxicity Activities of Various Extracts of Berberis vulgaris (Berberidaceae) and Its Active Principle Berberine |
title_full | Antifungal, Antileishmanial, and Cytotoxicity Activities of Various Extracts of Berberis vulgaris (Berberidaceae) and Its Active Principle Berberine |
title_fullStr | Antifungal, Antileishmanial, and Cytotoxicity Activities of Various Extracts of Berberis vulgaris (Berberidaceae) and Its Active Principle Berberine |
title_full_unstemmed | Antifungal, Antileishmanial, and Cytotoxicity Activities of Various Extracts of Berberis vulgaris (Berberidaceae) and Its Active Principle Berberine |
title_short | Antifungal, Antileishmanial, and Cytotoxicity Activities of Various Extracts of Berberis vulgaris (Berberidaceae) and Its Active Principle Berberine |
title_sort | antifungal, antileishmanial, and cytotoxicity activities of various extracts of berberis vulgaris (berberidaceae) and its active principle berberine |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3964876/ https://www.ncbi.nlm.nih.gov/pubmed/24977052 http://dx.doi.org/10.1155/2014/602436 |
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