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Prevention of amiodarone-induced cardiac toxicity in male BALB/c mice by a nutrient mixture

Amiodarone (Amio), a potent anti-arrhythmic drug, is associated with life-threatening pulmonary toxicity involving fibroses and inflammation. A unique nutrient mixture (NM) consisting of lysine, proline, ascorbic acid, N-acetyl cysteine and green tea extract has previously been shown to exhibit a br...

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Autores principales: ROOMI, M. WAHEED, ROOMI, NUSRATH WAHEED, KALINOVSKY, TATIANA, RATH, MATTHIAS, NIEDZWIECKI, ALEKSANDRA
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3964922/
https://www.ncbi.nlm.nih.gov/pubmed/24669263
http://dx.doi.org/10.3892/etm.2014.1518
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author ROOMI, M. WAHEED
ROOMI, NUSRATH WAHEED
KALINOVSKY, TATIANA
RATH, MATTHIAS
NIEDZWIECKI, ALEKSANDRA
author_facet ROOMI, M. WAHEED
ROOMI, NUSRATH WAHEED
KALINOVSKY, TATIANA
RATH, MATTHIAS
NIEDZWIECKI, ALEKSANDRA
author_sort ROOMI, M. WAHEED
collection PubMed
description Amiodarone (Amio), a potent anti-arrhythmic drug, is associated with life-threatening pulmonary toxicity involving fibroses and inflammation. A unique nutrient mixture (NM) consisting of lysine, proline, ascorbic acid, N-acetyl cysteine and green tea extract has previously been shown to exhibit a broad spectrum of pharmacological, therapeutic, cardiovascular and chemopreventive properties. The present study was undertaken to determine whether the NM exhibits preventive effects on Amio-induced cardiac toxicity. Six-week-old male BALB/c mice were divided into four groups (A–D) of six animals per group. Mice in groups A and C were fed a regular diet for three weeks, while the diets of the mice in groups B and D were supplemented with 1% NM during that period. After three weeks, the mice in groups C and D received daily Amio injections of 50 mg/kg body weight intraperitoneally for 4 days, whilst those in groups A and B received saline alone. At 24 h after the final dose, mice were sacrificed, blood was withdrawn and serum was collected for clinical chemistry of the heart enzymes creatine phosphokinase (CPK) and aspartate aminotransferase (AST). In addition, livers, kidneys, hearts and lungs were excised and weighed. No significant differences in weight gain were identified among the groups and liver, kidney, heart and lung weights were comparable in all four groups. Administration of Amio to group C resulted in a significant increase in serum CPK levels, whereas in NM-fed group D, the CPK levels were comparable to those in the saline injection groups, A and B. Amio administration also resulted in a significant increase in serum AST levels in group C, but not in the group D animals which exhibited similar levels to those of groups A and B. Therefore, the results indicate that NM has the potential to protect against Amio-induced cardiac toxicity.
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spelling pubmed-39649222014-03-25 Prevention of amiodarone-induced cardiac toxicity in male BALB/c mice by a nutrient mixture ROOMI, M. WAHEED ROOMI, NUSRATH WAHEED KALINOVSKY, TATIANA RATH, MATTHIAS NIEDZWIECKI, ALEKSANDRA Exp Ther Med Articles Amiodarone (Amio), a potent anti-arrhythmic drug, is associated with life-threatening pulmonary toxicity involving fibroses and inflammation. A unique nutrient mixture (NM) consisting of lysine, proline, ascorbic acid, N-acetyl cysteine and green tea extract has previously been shown to exhibit a broad spectrum of pharmacological, therapeutic, cardiovascular and chemopreventive properties. The present study was undertaken to determine whether the NM exhibits preventive effects on Amio-induced cardiac toxicity. Six-week-old male BALB/c mice were divided into four groups (A–D) of six animals per group. Mice in groups A and C were fed a regular diet for three weeks, while the diets of the mice in groups B and D were supplemented with 1% NM during that period. After three weeks, the mice in groups C and D received daily Amio injections of 50 mg/kg body weight intraperitoneally for 4 days, whilst those in groups A and B received saline alone. At 24 h after the final dose, mice were sacrificed, blood was withdrawn and serum was collected for clinical chemistry of the heart enzymes creatine phosphokinase (CPK) and aspartate aminotransferase (AST). In addition, livers, kidneys, hearts and lungs were excised and weighed. No significant differences in weight gain were identified among the groups and liver, kidney, heart and lung weights were comparable in all four groups. Administration of Amio to group C resulted in a significant increase in serum CPK levels, whereas in NM-fed group D, the CPK levels were comparable to those in the saline injection groups, A and B. Amio administration also resulted in a significant increase in serum AST levels in group C, but not in the group D animals which exhibited similar levels to those of groups A and B. Therefore, the results indicate that NM has the potential to protect against Amio-induced cardiac toxicity. D.A. Spandidos 2014-04 2014-02-03 /pmc/articles/PMC3964922/ /pubmed/24669263 http://dx.doi.org/10.3892/etm.2014.1518 Text en Copyright © 2014, Spandidos Publications http://creativecommons.org/licenses/by/3.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.
spellingShingle Articles
ROOMI, M. WAHEED
ROOMI, NUSRATH WAHEED
KALINOVSKY, TATIANA
RATH, MATTHIAS
NIEDZWIECKI, ALEKSANDRA
Prevention of amiodarone-induced cardiac toxicity in male BALB/c mice by a nutrient mixture
title Prevention of amiodarone-induced cardiac toxicity in male BALB/c mice by a nutrient mixture
title_full Prevention of amiodarone-induced cardiac toxicity in male BALB/c mice by a nutrient mixture
title_fullStr Prevention of amiodarone-induced cardiac toxicity in male BALB/c mice by a nutrient mixture
title_full_unstemmed Prevention of amiodarone-induced cardiac toxicity in male BALB/c mice by a nutrient mixture
title_short Prevention of amiodarone-induced cardiac toxicity in male BALB/c mice by a nutrient mixture
title_sort prevention of amiodarone-induced cardiac toxicity in male balb/c mice by a nutrient mixture
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3964922/
https://www.ncbi.nlm.nih.gov/pubmed/24669263
http://dx.doi.org/10.3892/etm.2014.1518
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