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M1/M2 macrophages and associated mechanisms in congenital bicuspid aortic valve stenosis
The aim of this study was to observe macrophage infiltration in congenital bicuspid aortic valve (CBAV) stenosis. M1/M2 macrophage distribution, inflammatory cytokine expression and the role of M1 macrophages during CBAV stenosis were also explored. The experimental and control groups comprised 30 s...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3965126/ https://www.ncbi.nlm.nih.gov/pubmed/24669254 http://dx.doi.org/10.3892/etm.2014.1529 |
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author | WANG, RUI CHEN, WEN MA, ZHIFEI LI, LIANGPENG CHEN, XIN |
author_facet | WANG, RUI CHEN, WEN MA, ZHIFEI LI, LIANGPENG CHEN, XIN |
author_sort | WANG, RUI |
collection | PubMed |
description | The aim of this study was to observe macrophage infiltration in congenital bicuspid aortic valve (CBAV) stenosis. M1/M2 macrophage distribution, inflammatory cytokine expression and the role of M1 macrophages during CBAV stenosis were also explored. The experimental and control groups comprised 30 severely stenotic CBAVs and 30 severely stenotic tricuspid aortic valves (TAVs), respectively. Histological and morphological changes were assessed using hematoxylin-eosin (HE) staining and mRNA levels of vascular endothelial growth factor (VEGF) were examined using the quantitative polymerase chain reaction. Nonspecific, M1 and M2 macrophages were monitored using cluster of differentiation (CD)68, inducible nitric oxide synthase (iNOS) and CD163 staining, respectively. Endothelial nitric oxide synthase (eNOS), interleukin (IL)-10, arginase (Arg)-1 and macrophage colony-stimulating factor (M-CSF) were also examined using immunohistochemical staining. Of note, HE staining revealed a higher cell density and neovascularization was more common in CBAVs than TAVs. At the mRNA level, VEGF expression was two-fold higher in CBAVs relative to that in TAVs (P=0.02). Furthermore, CD68 and iNOS were significantly higher in CBAVs compared with TAVs (P=0.029 and 0.021, respectively), while CD163 expression was lower in CBAVs (P=0.033). In addition, eNOS expression was higher and Arg-1, IL-10 and M-CSF expression were lower in CBAVs compared with TAVs (all P<0.0001). The present study suggested that CBAVs are associated with a higher total and M1 macrophage density and a lower M2 macrophage density than TAVs, and that M1 macrophage infiltration may contribute to calcification of CBAVs. |
format | Online Article Text |
id | pubmed-3965126 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-39651262014-03-25 M1/M2 macrophages and associated mechanisms in congenital bicuspid aortic valve stenosis WANG, RUI CHEN, WEN MA, ZHIFEI LI, LIANGPENG CHEN, XIN Exp Ther Med Articles The aim of this study was to observe macrophage infiltration in congenital bicuspid aortic valve (CBAV) stenosis. M1/M2 macrophage distribution, inflammatory cytokine expression and the role of M1 macrophages during CBAV stenosis were also explored. The experimental and control groups comprised 30 severely stenotic CBAVs and 30 severely stenotic tricuspid aortic valves (TAVs), respectively. Histological and morphological changes were assessed using hematoxylin-eosin (HE) staining and mRNA levels of vascular endothelial growth factor (VEGF) were examined using the quantitative polymerase chain reaction. Nonspecific, M1 and M2 macrophages were monitored using cluster of differentiation (CD)68, inducible nitric oxide synthase (iNOS) and CD163 staining, respectively. Endothelial nitric oxide synthase (eNOS), interleukin (IL)-10, arginase (Arg)-1 and macrophage colony-stimulating factor (M-CSF) were also examined using immunohistochemical staining. Of note, HE staining revealed a higher cell density and neovascularization was more common in CBAVs than TAVs. At the mRNA level, VEGF expression was two-fold higher in CBAVs relative to that in TAVs (P=0.02). Furthermore, CD68 and iNOS were significantly higher in CBAVs compared with TAVs (P=0.029 and 0.021, respectively), while CD163 expression was lower in CBAVs (P=0.033). In addition, eNOS expression was higher and Arg-1, IL-10 and M-CSF expression were lower in CBAVs compared with TAVs (all P<0.0001). The present study suggested that CBAVs are associated with a higher total and M1 macrophage density and a lower M2 macrophage density than TAVs, and that M1 macrophage infiltration may contribute to calcification of CBAVs. D.A. Spandidos 2014-04 2014-02-10 /pmc/articles/PMC3965126/ /pubmed/24669254 http://dx.doi.org/10.3892/etm.2014.1529 Text en Copyright © 2014, Spandidos Publications http://creativecommons.org/licenses/by/3.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited. |
spellingShingle | Articles WANG, RUI CHEN, WEN MA, ZHIFEI LI, LIANGPENG CHEN, XIN M1/M2 macrophages and associated mechanisms in congenital bicuspid aortic valve stenosis |
title | M1/M2 macrophages and associated mechanisms in congenital bicuspid aortic valve stenosis |
title_full | M1/M2 macrophages and associated mechanisms in congenital bicuspid aortic valve stenosis |
title_fullStr | M1/M2 macrophages and associated mechanisms in congenital bicuspid aortic valve stenosis |
title_full_unstemmed | M1/M2 macrophages and associated mechanisms in congenital bicuspid aortic valve stenosis |
title_short | M1/M2 macrophages and associated mechanisms in congenital bicuspid aortic valve stenosis |
title_sort | m1/m2 macrophages and associated mechanisms in congenital bicuspid aortic valve stenosis |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3965126/ https://www.ncbi.nlm.nih.gov/pubmed/24669254 http://dx.doi.org/10.3892/etm.2014.1529 |
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