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Antimicrobial Peptides: New Recognition Molecules for Detecting Botulinum Toxins

Many organisms secrete antimicrobial peptides (AMPs) for protection against harmful microbes. The present study describes detection of botulinum neurotoxoids A, B and E using AMPs as recognition elements in an array biosensor. While AMP affinities were similar to those for anti-botulinum antibodies,...

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Autores principales: Kulagina, Nadezhda V., Anderson, George P., Ligler, Frances S., Shaffer, Kara M., Taitt, Chris Rowe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3965214/
https://www.ncbi.nlm.nih.gov/pubmed/28903262
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author Kulagina, Nadezhda V.
Anderson, George P.
Ligler, Frances S.
Shaffer, Kara M.
Taitt, Chris Rowe
author_facet Kulagina, Nadezhda V.
Anderson, George P.
Ligler, Frances S.
Shaffer, Kara M.
Taitt, Chris Rowe
author_sort Kulagina, Nadezhda V.
collection PubMed
description Many organisms secrete antimicrobial peptides (AMPs) for protection against harmful microbes. The present study describes detection of botulinum neurotoxoids A, B and E using AMPs as recognition elements in an array biosensor. While AMP affinities were similar to those for anti-botulinum antibodies, differences in binding patterns were observed and can potentially be used for identification of toxoid serotype. Furthermore, some AMPs also demonstrated superior detection sensitivity compared to antibodies: toxoid A could be detected at 3.5 LD(50) of the active toxin in a 75-min assay, whereas toxoids B and E were detected at 14 and 80 LD(50) for their respective toxins.
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spelling pubmed-39652142014-03-25 Antimicrobial Peptides: New Recognition Molecules for Detecting Botulinum Toxins Kulagina, Nadezhda V. Anderson, George P. Ligler, Frances S. Shaffer, Kara M. Taitt, Chris Rowe Sensors (Basel) Full Research Paper Many organisms secrete antimicrobial peptides (AMPs) for protection against harmful microbes. The present study describes detection of botulinum neurotoxoids A, B and E using AMPs as recognition elements in an array biosensor. While AMP affinities were similar to those for anti-botulinum antibodies, differences in binding patterns were observed and can potentially be used for identification of toxoid serotype. Furthermore, some AMPs also demonstrated superior detection sensitivity compared to antibodies: toxoid A could be detected at 3.5 LD(50) of the active toxin in a 75-min assay, whereas toxoids B and E were detected at 14 and 80 LD(50) for their respective toxins. Molecular Diversity Preservation International (MDPI) 2007-11-16 /pmc/articles/PMC3965214/ /pubmed/28903262 Text en © 2007 by MDPI (http://www.mdpi.org). Reproduction is permitted for noncommercial purposes.
spellingShingle Full Research Paper
Kulagina, Nadezhda V.
Anderson, George P.
Ligler, Frances S.
Shaffer, Kara M.
Taitt, Chris Rowe
Antimicrobial Peptides: New Recognition Molecules for Detecting Botulinum Toxins
title Antimicrobial Peptides: New Recognition Molecules for Detecting Botulinum Toxins
title_full Antimicrobial Peptides: New Recognition Molecules for Detecting Botulinum Toxins
title_fullStr Antimicrobial Peptides: New Recognition Molecules for Detecting Botulinum Toxins
title_full_unstemmed Antimicrobial Peptides: New Recognition Molecules for Detecting Botulinum Toxins
title_short Antimicrobial Peptides: New Recognition Molecules for Detecting Botulinum Toxins
title_sort antimicrobial peptides: new recognition molecules for detecting botulinum toxins
topic Full Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3965214/
https://www.ncbi.nlm.nih.gov/pubmed/28903262
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