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Antimicrobial Peptides: New Recognition Molecules for Detecting Botulinum Toxins
Many organisms secrete antimicrobial peptides (AMPs) for protection against harmful microbes. The present study describes detection of botulinum neurotoxoids A, B and E using AMPs as recognition elements in an array biosensor. While AMP affinities were similar to those for anti-botulinum antibodies,...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Molecular Diversity Preservation International (MDPI)
2007
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3965214/ https://www.ncbi.nlm.nih.gov/pubmed/28903262 |
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author | Kulagina, Nadezhda V. Anderson, George P. Ligler, Frances S. Shaffer, Kara M. Taitt, Chris Rowe |
author_facet | Kulagina, Nadezhda V. Anderson, George P. Ligler, Frances S. Shaffer, Kara M. Taitt, Chris Rowe |
author_sort | Kulagina, Nadezhda V. |
collection | PubMed |
description | Many organisms secrete antimicrobial peptides (AMPs) for protection against harmful microbes. The present study describes detection of botulinum neurotoxoids A, B and E using AMPs as recognition elements in an array biosensor. While AMP affinities were similar to those for anti-botulinum antibodies, differences in binding patterns were observed and can potentially be used for identification of toxoid serotype. Furthermore, some AMPs also demonstrated superior detection sensitivity compared to antibodies: toxoid A could be detected at 3.5 LD(50) of the active toxin in a 75-min assay, whereas toxoids B and E were detected at 14 and 80 LD(50) for their respective toxins. |
format | Online Article Text |
id | pubmed-3965214 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2007 |
publisher | Molecular Diversity Preservation International (MDPI) |
record_format | MEDLINE/PubMed |
spelling | pubmed-39652142014-03-25 Antimicrobial Peptides: New Recognition Molecules for Detecting Botulinum Toxins Kulagina, Nadezhda V. Anderson, George P. Ligler, Frances S. Shaffer, Kara M. Taitt, Chris Rowe Sensors (Basel) Full Research Paper Many organisms secrete antimicrobial peptides (AMPs) for protection against harmful microbes. The present study describes detection of botulinum neurotoxoids A, B and E using AMPs as recognition elements in an array biosensor. While AMP affinities were similar to those for anti-botulinum antibodies, differences in binding patterns were observed and can potentially be used for identification of toxoid serotype. Furthermore, some AMPs also demonstrated superior detection sensitivity compared to antibodies: toxoid A could be detected at 3.5 LD(50) of the active toxin in a 75-min assay, whereas toxoids B and E were detected at 14 and 80 LD(50) for their respective toxins. Molecular Diversity Preservation International (MDPI) 2007-11-16 /pmc/articles/PMC3965214/ /pubmed/28903262 Text en © 2007 by MDPI (http://www.mdpi.org). Reproduction is permitted for noncommercial purposes. |
spellingShingle | Full Research Paper Kulagina, Nadezhda V. Anderson, George P. Ligler, Frances S. Shaffer, Kara M. Taitt, Chris Rowe Antimicrobial Peptides: New Recognition Molecules for Detecting Botulinum Toxins |
title | Antimicrobial Peptides: New Recognition Molecules for Detecting Botulinum Toxins |
title_full | Antimicrobial Peptides: New Recognition Molecules for Detecting Botulinum Toxins |
title_fullStr | Antimicrobial Peptides: New Recognition Molecules for Detecting Botulinum Toxins |
title_full_unstemmed | Antimicrobial Peptides: New Recognition Molecules for Detecting Botulinum Toxins |
title_short | Antimicrobial Peptides: New Recognition Molecules for Detecting Botulinum Toxins |
title_sort | antimicrobial peptides: new recognition molecules for detecting botulinum toxins |
topic | Full Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3965214/ https://www.ncbi.nlm.nih.gov/pubmed/28903262 |
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