Electrochemical Interrogation of Interactions between Surface-Confined DNA and Methylene Blue

In this work, we reported a systematic investigation on the interactions between methylene blue (MB) and surface-confined DNA by using electrochemical methods. We demonstrated that the redox potential of MB and binding and dissociation kinetics of MB to DNA differed significantly for single-stranded...

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Detalles Bibliográficos
Autores principales: Pan, Dun, Zuo, Xiaolei, Wan, Ying, Wang, Lihua, Zhang, Jiong, Song, Shiping, Fan, Chunhai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2007
Materias:
Full Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3965235/
https://www.ncbi.nlm.nih.gov/pubmed/28903254
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author Pan, Dun
Zuo, Xiaolei
Wan, Ying
Wang, Lihua
Zhang, Jiong
Song, Shiping
Fan, Chunhai
author_facet Pan, Dun
Zuo, Xiaolei
Wan, Ying
Wang, Lihua
Zhang, Jiong
Song, Shiping
Fan, Chunhai
author_sort Pan, Dun
collection PubMed
description In this work, we reported a systematic investigation on the interactions between methylene blue (MB) and surface-confined DNA by using electrochemical methods. We demonstrated that the redox potential of MB and binding and dissociation kinetics of MB to DNA differed significantly for single-stranded DNA (ss-DNA) and double-stranded DNA (ds-DNA) immobilized on gold electrodes. This was possibly due to the different binding mechanism between MB and ss- or ds-DNA. This work might provide useful information for developing MB-based sequence-specific electrochemical DNA sensors.
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spelling pubmed-39652352014-03-25 Electrochemical Interrogation of Interactions between Surface-Confined DNA and Methylene Blue Pan, Dun Zuo, Xiaolei Wan, Ying Wang, Lihua Zhang, Jiong Song, Shiping Fan, Chunhai Sensors (Basel) Full Research Paper In this work, we reported a systematic investigation on the interactions between methylene blue (MB) and surface-confined DNA by using electrochemical methods. We demonstrated that the redox potential of MB and binding and dissociation kinetics of MB to DNA differed significantly for single-stranded DNA (ss-DNA) and double-stranded DNA (ds-DNA) immobilized on gold electrodes. This was possibly due to the different binding mechanism between MB and ss- or ds-DNA. This work might provide useful information for developing MB-based sequence-specific electrochemical DNA sensors. Molecular Diversity Preservation International (MDPI) 2007-11-12 /pmc/articles/PMC3965235/ /pubmed/28903254 Text en © 2007 by MDPI (http://www.mdpi.org). Reproduction is permitted for noncommercial purposes.
spellingShingle Full Research Paper
Pan, Dun
Zuo, Xiaolei
Wan, Ying
Wang, Lihua
Zhang, Jiong
Song, Shiping
Fan, Chunhai
Electrochemical Interrogation of Interactions between Surface-Confined DNA and Methylene Blue
title Electrochemical Interrogation of Interactions between Surface-Confined DNA and Methylene Blue
title_full Electrochemical Interrogation of Interactions between Surface-Confined DNA and Methylene Blue
title_fullStr Electrochemical Interrogation of Interactions between Surface-Confined DNA and Methylene Blue
title_full_unstemmed Electrochemical Interrogation of Interactions between Surface-Confined DNA and Methylene Blue
title_short Electrochemical Interrogation of Interactions between Surface-Confined DNA and Methylene Blue
title_sort electrochemical interrogation of interactions between surface-confined dna and methylene blue
topic Full Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3965235/
https://www.ncbi.nlm.nih.gov/pubmed/28903254
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AT zuoxiaolei electrochemicalinterrogationofinteractionsbetweensurfaceconfineddnaandmethyleneblue
AT wanying electrochemicalinterrogationofinteractionsbetweensurfaceconfineddnaandmethyleneblue
AT wanglihua electrochemicalinterrogationofinteractionsbetweensurfaceconfineddnaandmethyleneblue
AT zhangjiong electrochemicalinterrogationofinteractionsbetweensurfaceconfineddnaandmethyleneblue
AT songshiping electrochemicalinterrogationofinteractionsbetweensurfaceconfineddnaandmethyleneblue
AT fanchunhai electrochemicalinterrogationofinteractionsbetweensurfaceconfineddnaandmethyleneblue

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