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The Effects of Super-Flux (High Performance) Dialyzer on Plasma Glycosylated Pro-B-Type Natriuretic Peptide (proBNP) and Glycosylated N-Terminal proBNP in End-Stage Renal Disease Patients on Dialysis

BACKGROUND: Plasma BNP levels are predictive of prognosis in hemodialysis patients. However, recent studies showed that the current BNP immunoassay cross-reacts with glycosylated proBNP, and the NT-proBNP assay underestimates glycosylated NT-proBNP. In addition, the recently developed high performan...

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Autores principales: Nakagawa, Yasuaki, Nishikimi, Toshio, Kuwahara, Koichiro, Yasuno, Shinji, Kinoshita, Hideyuki, Kuwabara, Yoshihiro, Nakao, Kazuhiro, Minami, Takeya, Yamada, Chinatsu, Ueshima, Kenji, Ikeda, Yoshihiro, Okamoto, Hiroyuki, Horii, Kazukiyo, Nagata, Kiyoshi, Kangawa, Kenji, Minamino, Naoto, Nakao, Kazuwa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3965412/
https://www.ncbi.nlm.nih.gov/pubmed/24667631
http://dx.doi.org/10.1371/journal.pone.0092314
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author Nakagawa, Yasuaki
Nishikimi, Toshio
Kuwahara, Koichiro
Yasuno, Shinji
Kinoshita, Hideyuki
Kuwabara, Yoshihiro
Nakao, Kazuhiro
Minami, Takeya
Yamada, Chinatsu
Ueshima, Kenji
Ikeda, Yoshihiro
Okamoto, Hiroyuki
Horii, Kazukiyo
Nagata, Kiyoshi
Kangawa, Kenji
Minamino, Naoto
Nakao, Kazuwa
author_facet Nakagawa, Yasuaki
Nishikimi, Toshio
Kuwahara, Koichiro
Yasuno, Shinji
Kinoshita, Hideyuki
Kuwabara, Yoshihiro
Nakao, Kazuhiro
Minami, Takeya
Yamada, Chinatsu
Ueshima, Kenji
Ikeda, Yoshihiro
Okamoto, Hiroyuki
Horii, Kazukiyo
Nagata, Kiyoshi
Kangawa, Kenji
Minamino, Naoto
Nakao, Kazuwa
author_sort Nakagawa, Yasuaki
collection PubMed
description BACKGROUND: Plasma BNP levels are predictive of prognosis in hemodialysis patients. However, recent studies showed that the current BNP immunoassay cross-reacts with glycosylated proBNP, and the NT-proBNP assay underestimates glycosylated NT-proBNP. In addition, the recently developed high performance dialyzer removes medium-sized molecular solutes such as β2-microgloburin. We therefore investigated the effects of high performance dialysis on measured levels of glycosylated proBNP, glycosylated NT-proBNP and other BNP-related peptides in end-stage renal disease (ESRD) patients on hemodialysis. METHOD: The relationships between clinical parameters and BNP-related molecule were also investigated. We used our newly developed immunoassay to measure plasma total BNP and proBNP in 105 normal subjects and 36 ESRD patients before and after hemodialysis. Plasma NT-proBNP was measured using Elecsys II after treatment with or without deglycosylating enzymes. We also measured plasma ANP and cGMP using radioimmunoassays. RESULTS: All the measured BNP-related peptides were significantly higher in ESRD patients than healthy subjects. Total BNP (−38.9%), proBNP (−29.7%), glycoNT-proBNP (−45.5%), nonglycoNT-proBNP (−53.4%), ANP (−50.4%) and cGMP (−72.1%) were all significantly reduced after hemodialysis, and the magnitude of the reduction appeared molecular weight- dependent. Both the proBNP/total BNP and glycoNT-proBNP/nonglycoNT-proBNP ratios were increased after hemodialysis. The former correlated positively with hemodialysis vintage and negatively with systolic blood pressure, while the latter correlated positively with parathyroid hormone levels. CONCLUSION: These results suggest that hemodialysis using super-flux dialyzer removes BNP-related peptides in a nearly molecular weight-dependent manner. The ProBNP/total BNP and glycoNT-proBNP/nonglycoNT-proBNP ratios appear to be influenced by hemodialysis-related parameters in ESRD patients on hemodialysis.
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spelling pubmed-39654122014-03-27 The Effects of Super-Flux (High Performance) Dialyzer on Plasma Glycosylated Pro-B-Type Natriuretic Peptide (proBNP) and Glycosylated N-Terminal proBNP in End-Stage Renal Disease Patients on Dialysis Nakagawa, Yasuaki Nishikimi, Toshio Kuwahara, Koichiro Yasuno, Shinji Kinoshita, Hideyuki Kuwabara, Yoshihiro Nakao, Kazuhiro Minami, Takeya Yamada, Chinatsu Ueshima, Kenji Ikeda, Yoshihiro Okamoto, Hiroyuki Horii, Kazukiyo Nagata, Kiyoshi Kangawa, Kenji Minamino, Naoto Nakao, Kazuwa PLoS One Research Article BACKGROUND: Plasma BNP levels are predictive of prognosis in hemodialysis patients. However, recent studies showed that the current BNP immunoassay cross-reacts with glycosylated proBNP, and the NT-proBNP assay underestimates glycosylated NT-proBNP. In addition, the recently developed high performance dialyzer removes medium-sized molecular solutes such as β2-microgloburin. We therefore investigated the effects of high performance dialysis on measured levels of glycosylated proBNP, glycosylated NT-proBNP and other BNP-related peptides in end-stage renal disease (ESRD) patients on hemodialysis. METHOD: The relationships between clinical parameters and BNP-related molecule were also investigated. We used our newly developed immunoassay to measure plasma total BNP and proBNP in 105 normal subjects and 36 ESRD patients before and after hemodialysis. Plasma NT-proBNP was measured using Elecsys II after treatment with or without deglycosylating enzymes. We also measured plasma ANP and cGMP using radioimmunoassays. RESULTS: All the measured BNP-related peptides were significantly higher in ESRD patients than healthy subjects. Total BNP (−38.9%), proBNP (−29.7%), glycoNT-proBNP (−45.5%), nonglycoNT-proBNP (−53.4%), ANP (−50.4%) and cGMP (−72.1%) were all significantly reduced after hemodialysis, and the magnitude of the reduction appeared molecular weight- dependent. Both the proBNP/total BNP and glycoNT-proBNP/nonglycoNT-proBNP ratios were increased after hemodialysis. The former correlated positively with hemodialysis vintage and negatively with systolic blood pressure, while the latter correlated positively with parathyroid hormone levels. CONCLUSION: These results suggest that hemodialysis using super-flux dialyzer removes BNP-related peptides in a nearly molecular weight-dependent manner. The ProBNP/total BNP and glycoNT-proBNP/nonglycoNT-proBNP ratios appear to be influenced by hemodialysis-related parameters in ESRD patients on hemodialysis. Public Library of Science 2014-03-25 /pmc/articles/PMC3965412/ /pubmed/24667631 http://dx.doi.org/10.1371/journal.pone.0092314 Text en © 2014 Nakagawa et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Nakagawa, Yasuaki
Nishikimi, Toshio
Kuwahara, Koichiro
Yasuno, Shinji
Kinoshita, Hideyuki
Kuwabara, Yoshihiro
Nakao, Kazuhiro
Minami, Takeya
Yamada, Chinatsu
Ueshima, Kenji
Ikeda, Yoshihiro
Okamoto, Hiroyuki
Horii, Kazukiyo
Nagata, Kiyoshi
Kangawa, Kenji
Minamino, Naoto
Nakao, Kazuwa
The Effects of Super-Flux (High Performance) Dialyzer on Plasma Glycosylated Pro-B-Type Natriuretic Peptide (proBNP) and Glycosylated N-Terminal proBNP in End-Stage Renal Disease Patients on Dialysis
title The Effects of Super-Flux (High Performance) Dialyzer on Plasma Glycosylated Pro-B-Type Natriuretic Peptide (proBNP) and Glycosylated N-Terminal proBNP in End-Stage Renal Disease Patients on Dialysis
title_full The Effects of Super-Flux (High Performance) Dialyzer on Plasma Glycosylated Pro-B-Type Natriuretic Peptide (proBNP) and Glycosylated N-Terminal proBNP in End-Stage Renal Disease Patients on Dialysis
title_fullStr The Effects of Super-Flux (High Performance) Dialyzer on Plasma Glycosylated Pro-B-Type Natriuretic Peptide (proBNP) and Glycosylated N-Terminal proBNP in End-Stage Renal Disease Patients on Dialysis
title_full_unstemmed The Effects of Super-Flux (High Performance) Dialyzer on Plasma Glycosylated Pro-B-Type Natriuretic Peptide (proBNP) and Glycosylated N-Terminal proBNP in End-Stage Renal Disease Patients on Dialysis
title_short The Effects of Super-Flux (High Performance) Dialyzer on Plasma Glycosylated Pro-B-Type Natriuretic Peptide (proBNP) and Glycosylated N-Terminal proBNP in End-Stage Renal Disease Patients on Dialysis
title_sort effects of super-flux (high performance) dialyzer on plasma glycosylated pro-b-type natriuretic peptide (probnp) and glycosylated n-terminal probnp in end-stage renal disease patients on dialysis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3965412/
https://www.ncbi.nlm.nih.gov/pubmed/24667631
http://dx.doi.org/10.1371/journal.pone.0092314
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