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Overexpression of OsMYB48-1, a Novel MYB-Related Transcription Factor, Enhances Drought and Salinity Tolerance in Rice

MYB-type transcription factors (TFs) play essential roles in plant growth, development and respond to environmental stresses. Role of MYB-related TFs of rice in drought stress tolerance is not well documented. Here, we report the isolation and characterization of a novel MYB-related TF, OsMYB48-1, o...

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Autores principales: Xiong, Haiyan, Li, Jinjie, Liu, Pengli, Duan, Junzhi, Zhao, Yan, Guo, Xiao, Li, Yang, Zhang, Hongliang, Ali, Jauhar, Li, Zichao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3965499/
https://www.ncbi.nlm.nih.gov/pubmed/24667379
http://dx.doi.org/10.1371/journal.pone.0092913
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author Xiong, Haiyan
Li, Jinjie
Liu, Pengli
Duan, Junzhi
Zhao, Yan
Guo, Xiao
Li, Yang
Zhang, Hongliang
Ali, Jauhar
Li, Zichao
author_facet Xiong, Haiyan
Li, Jinjie
Liu, Pengli
Duan, Junzhi
Zhao, Yan
Guo, Xiao
Li, Yang
Zhang, Hongliang
Ali, Jauhar
Li, Zichao
author_sort Xiong, Haiyan
collection PubMed
description MYB-type transcription factors (TFs) play essential roles in plant growth, development and respond to environmental stresses. Role of MYB-related TFs of rice in drought stress tolerance is not well documented. Here, we report the isolation and characterization of a novel MYB-related TF, OsMYB48-1, of rice. Expression of OsMYB48-1 was strongly induced by polyethylene glycol (PEG), abscisic acid (ABA), H(2)O(2), and dehydration, while being slightly induced by high salinity and cold treatment. The OsMYB48-1 protein was localized in the nucleus with transactivation activity at the C terminus. Overexpression of OsMYB48-1 in rice significantly improved tolerance to simulated drought and salinity stresses caused by mannitol, PEG, and NaCl, respectively, and drought stress was caused by drying the soil. In contrast to wild type plants, the overexpression lines exhibited reduced rate of water loss, lower malondialdehyde (MDA) content and higher proline content under stress conditions. Moreover, overexpression plants were hypersensitive to ABA at both germination and post-germination stages and accumulated more endogenous ABA under drought stress conditions. Further studies demonstrated that overexpression of OsMYB48-1 could regulate the expression of some ABA biosynthesis genes (OsNCED4, OsNCED5), early signaling genes (OsPP2C68, OSRK1) and late responsive genes (RAB21, OsLEA3, RAB16C and RAB16D) under drought stress conditions. Collectively, these results suggested that OsMYB48-1 functions as a novel MYB-related TF which plays a positive role in drought and salinity tolerance by regulating stress-induced ABA synthesis.
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spelling pubmed-39654992014-03-27 Overexpression of OsMYB48-1, a Novel MYB-Related Transcription Factor, Enhances Drought and Salinity Tolerance in Rice Xiong, Haiyan Li, Jinjie Liu, Pengli Duan, Junzhi Zhao, Yan Guo, Xiao Li, Yang Zhang, Hongliang Ali, Jauhar Li, Zichao PLoS One Research Article MYB-type transcription factors (TFs) play essential roles in plant growth, development and respond to environmental stresses. Role of MYB-related TFs of rice in drought stress tolerance is not well documented. Here, we report the isolation and characterization of a novel MYB-related TF, OsMYB48-1, of rice. Expression of OsMYB48-1 was strongly induced by polyethylene glycol (PEG), abscisic acid (ABA), H(2)O(2), and dehydration, while being slightly induced by high salinity and cold treatment. The OsMYB48-1 protein was localized in the nucleus with transactivation activity at the C terminus. Overexpression of OsMYB48-1 in rice significantly improved tolerance to simulated drought and salinity stresses caused by mannitol, PEG, and NaCl, respectively, and drought stress was caused by drying the soil. In contrast to wild type plants, the overexpression lines exhibited reduced rate of water loss, lower malondialdehyde (MDA) content and higher proline content under stress conditions. Moreover, overexpression plants were hypersensitive to ABA at both germination and post-germination stages and accumulated more endogenous ABA under drought stress conditions. Further studies demonstrated that overexpression of OsMYB48-1 could regulate the expression of some ABA biosynthesis genes (OsNCED4, OsNCED5), early signaling genes (OsPP2C68, OSRK1) and late responsive genes (RAB21, OsLEA3, RAB16C and RAB16D) under drought stress conditions. Collectively, these results suggested that OsMYB48-1 functions as a novel MYB-related TF which plays a positive role in drought and salinity tolerance by regulating stress-induced ABA synthesis. Public Library of Science 2014-03-25 /pmc/articles/PMC3965499/ /pubmed/24667379 http://dx.doi.org/10.1371/journal.pone.0092913 Text en © 2014 Xiong et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Xiong, Haiyan
Li, Jinjie
Liu, Pengli
Duan, Junzhi
Zhao, Yan
Guo, Xiao
Li, Yang
Zhang, Hongliang
Ali, Jauhar
Li, Zichao
Overexpression of OsMYB48-1, a Novel MYB-Related Transcription Factor, Enhances Drought and Salinity Tolerance in Rice
title Overexpression of OsMYB48-1, a Novel MYB-Related Transcription Factor, Enhances Drought and Salinity Tolerance in Rice
title_full Overexpression of OsMYB48-1, a Novel MYB-Related Transcription Factor, Enhances Drought and Salinity Tolerance in Rice
title_fullStr Overexpression of OsMYB48-1, a Novel MYB-Related Transcription Factor, Enhances Drought and Salinity Tolerance in Rice
title_full_unstemmed Overexpression of OsMYB48-1, a Novel MYB-Related Transcription Factor, Enhances Drought and Salinity Tolerance in Rice
title_short Overexpression of OsMYB48-1, a Novel MYB-Related Transcription Factor, Enhances Drought and Salinity Tolerance in Rice
title_sort overexpression of osmyb48-1, a novel myb-related transcription factor, enhances drought and salinity tolerance in rice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3965499/
https://www.ncbi.nlm.nih.gov/pubmed/24667379
http://dx.doi.org/10.1371/journal.pone.0092913
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