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Lipoprotein lipase activity is required for cardiac lipid droplet production
The rodent heart accumulates TGs and lipid droplets during fasting. The sources of heart lipids could be either FFAs liberated from adipose tissue or FAs from lipoprotein-associated TGs via the action of lipoprotein lipase (LpL). Because circulating levels of FFAs increase during fasting, it has bee...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The American Society for Biochemistry and Molecular Biology
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3966699/ https://www.ncbi.nlm.nih.gov/pubmed/24493834 http://dx.doi.org/10.1194/jlr.M043471 |
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author | Trent, Chad M. Yu, Shuiqing Hu, Yunying Skoller, Nathan Huggins, Lesley A. Homma, Shunichi Goldberg, Ira J. |
author_facet | Trent, Chad M. Yu, Shuiqing Hu, Yunying Skoller, Nathan Huggins, Lesley A. Homma, Shunichi Goldberg, Ira J. |
author_sort | Trent, Chad M. |
collection | PubMed |
description | The rodent heart accumulates TGs and lipid droplets during fasting. The sources of heart lipids could be either FFAs liberated from adipose tissue or FAs from lipoprotein-associated TGs via the action of lipoprotein lipase (LpL). Because circulating levels of FFAs increase during fasting, it has been assumed that albumin transported FFAs are the source of lipids within heart lipid droplets. We studied mice with three genetic mutations: peroxisomal proliferator-activated receptor α deficiency, cluster of differentiation 36 (CD36) deficiency, and heart-specific LpL deletion. All three genetically altered groups of mice had defective accumulation of lipid droplet TGs. Moreover, hearts from mice treated with poloxamer 407, an inhibitor of lipoprotein TG lipolysis, also failed to accumulate TGs, despite increased uptake of FFAs. TG storage did not impair maximal cardiac function as measured by stress echocardiography. Thus, LpL hydrolysis of circulating lipoproteins is required for the accumulation of lipids in the heart of fasting mice. |
format | Online Article Text |
id | pubmed-3966699 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | The American Society for Biochemistry and Molecular Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-39666992014-04-03 Lipoprotein lipase activity is required for cardiac lipid droplet production Trent, Chad M. Yu, Shuiqing Hu, Yunying Skoller, Nathan Huggins, Lesley A. Homma, Shunichi Goldberg, Ira J. J Lipid Res Research Articles The rodent heart accumulates TGs and lipid droplets during fasting. The sources of heart lipids could be either FFAs liberated from adipose tissue or FAs from lipoprotein-associated TGs via the action of lipoprotein lipase (LpL). Because circulating levels of FFAs increase during fasting, it has been assumed that albumin transported FFAs are the source of lipids within heart lipid droplets. We studied mice with three genetic mutations: peroxisomal proliferator-activated receptor α deficiency, cluster of differentiation 36 (CD36) deficiency, and heart-specific LpL deletion. All three genetically altered groups of mice had defective accumulation of lipid droplet TGs. Moreover, hearts from mice treated with poloxamer 407, an inhibitor of lipoprotein TG lipolysis, also failed to accumulate TGs, despite increased uptake of FFAs. TG storage did not impair maximal cardiac function as measured by stress echocardiography. Thus, LpL hydrolysis of circulating lipoproteins is required for the accumulation of lipids in the heart of fasting mice. The American Society for Biochemistry and Molecular Biology 2014-04 /pmc/articles/PMC3966699/ /pubmed/24493834 http://dx.doi.org/10.1194/jlr.M043471 Text en Copyright © 2014 by the American Society for Biochemistry and Molecular Biology, Inc. http://creativecommons.org/licenses/by/3.0/ Author's Choice—Final version full access. Creative Commons Attribution Unported License (http://creativecommons.org/licenses/by/3.0/) applies to Author Choice Articles |
spellingShingle | Research Articles Trent, Chad M. Yu, Shuiqing Hu, Yunying Skoller, Nathan Huggins, Lesley A. Homma, Shunichi Goldberg, Ira J. Lipoprotein lipase activity is required for cardiac lipid droplet production |
title | Lipoprotein lipase activity is required for cardiac lipid droplet production |
title_full | Lipoprotein lipase activity is required for cardiac lipid droplet production |
title_fullStr | Lipoprotein lipase activity is required for cardiac lipid droplet production |
title_full_unstemmed | Lipoprotein lipase activity is required for cardiac lipid droplet production |
title_short | Lipoprotein lipase activity is required for cardiac lipid droplet production |
title_sort | lipoprotein lipase activity is required for cardiac lipid droplet production |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3966699/ https://www.ncbi.nlm.nih.gov/pubmed/24493834 http://dx.doi.org/10.1194/jlr.M043471 |
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