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Class I BASIC PENTACYSTEINE factors regulate HOMEOBOX genes involved in meristem size maintenance
The BASIC PENTACYSTEINE (BCP) family is a poorly characterized plant transcription factor family of GAGA BINDING PROTEINS. In Arabidopsis, there are seven members (BPC1–7) that are broadly expressed, and they can potentially bind more than 3000 Arabidopsis GAGA-repeat-containing genes. To date, BPCs...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3967085/ https://www.ncbi.nlm.nih.gov/pubmed/24482368 http://dx.doi.org/10.1093/jxb/eru003 |
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author | Simonini, Sara Kater, Martin M. |
author_facet | Simonini, Sara Kater, Martin M. |
author_sort | Simonini, Sara |
collection | PubMed |
description | The BASIC PENTACYSTEINE (BCP) family is a poorly characterized plant transcription factor family of GAGA BINDING PROTEINS. In Arabidopsis, there are seven members (BPC1–7) that are broadly expressed, and they can potentially bind more than 3000 Arabidopsis GAGA-repeat-containing genes. To date, BPCs are known to be direct regulators of the INNER NO OUTER (INO), SEEDSTICK (STK), and LEAFY COTYLEDON 2 (LEC2) genes. Because of the high functional redundancy, neither single knockout nor double bpc mutant combinations cause aberrant phenotypes. The bpc1-2 bpc2 bpc3 triple mutant shows several pleiotropic developmental defects, including enlargement of the inflorescence meristem and flowers with supernumerary floral organs. Here, we demonstrated through expression analysis and chromatin immunoprecipitation assays that this phenotype is probably due to deregulation of the expression of the SHOOTMERISTEMLESS (STM) and BREVIPEDICELLUS/KNAT1 (BP) genes, which are both direct targets of BPCs. Moreover, we assigned a role to BPCs in the fine regulation of the cytokinin content in the meristem, as both ISOPENTENYLTRANSFERASE 7 (IPT7) and ARABIDOPSIS RESPONSE REGULATOR 7 (ARR7) genes were shown to be overexpressed in the bpc1-2 bpc2 bpc3 triple mutant. |
format | Online Article Text |
id | pubmed-3967085 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-39670852014-06-18 Class I BASIC PENTACYSTEINE factors regulate HOMEOBOX genes involved in meristem size maintenance Simonini, Sara Kater, Martin M. J Exp Bot Research Paper The BASIC PENTACYSTEINE (BCP) family is a poorly characterized plant transcription factor family of GAGA BINDING PROTEINS. In Arabidopsis, there are seven members (BPC1–7) that are broadly expressed, and they can potentially bind more than 3000 Arabidopsis GAGA-repeat-containing genes. To date, BPCs are known to be direct regulators of the INNER NO OUTER (INO), SEEDSTICK (STK), and LEAFY COTYLEDON 2 (LEC2) genes. Because of the high functional redundancy, neither single knockout nor double bpc mutant combinations cause aberrant phenotypes. The bpc1-2 bpc2 bpc3 triple mutant shows several pleiotropic developmental defects, including enlargement of the inflorescence meristem and flowers with supernumerary floral organs. Here, we demonstrated through expression analysis and chromatin immunoprecipitation assays that this phenotype is probably due to deregulation of the expression of the SHOOTMERISTEMLESS (STM) and BREVIPEDICELLUS/KNAT1 (BP) genes, which are both direct targets of BPCs. Moreover, we assigned a role to BPCs in the fine regulation of the cytokinin content in the meristem, as both ISOPENTENYLTRANSFERASE 7 (IPT7) and ARABIDOPSIS RESPONSE REGULATOR 7 (ARR7) genes were shown to be overexpressed in the bpc1-2 bpc2 bpc3 triple mutant. Oxford University Press 2014-04 2014-01-30 /pmc/articles/PMC3967085/ /pubmed/24482368 http://dx.doi.org/10.1093/jxb/eru003 Text en © The Author 2014. Published by Oxford University Press on behalf of the Society for Experimental Biology. http://creativecommons.org/licenses/by/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Paper Simonini, Sara Kater, Martin M. Class I BASIC PENTACYSTEINE factors regulate HOMEOBOX genes involved in meristem size maintenance |
title | Class I BASIC PENTACYSTEINE factors regulate HOMEOBOX genes involved in meristem size maintenance |
title_full | Class I BASIC PENTACYSTEINE factors regulate HOMEOBOX genes involved in meristem size maintenance |
title_fullStr | Class I BASIC PENTACYSTEINE factors regulate HOMEOBOX genes involved in meristem size maintenance |
title_full_unstemmed | Class I BASIC PENTACYSTEINE factors regulate HOMEOBOX genes involved in meristem size maintenance |
title_short | Class I BASIC PENTACYSTEINE factors regulate HOMEOBOX genes involved in meristem size maintenance |
title_sort | class i basic pentacysteine factors regulate homeobox genes involved in meristem size maintenance |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3967085/ https://www.ncbi.nlm.nih.gov/pubmed/24482368 http://dx.doi.org/10.1093/jxb/eru003 |
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