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Class I BASIC PENTACYSTEINE factors regulate HOMEOBOX genes involved in meristem size maintenance

The BASIC PENTACYSTEINE (BCP) family is a poorly characterized plant transcription factor family of GAGA BINDING PROTEINS. In Arabidopsis, there are seven members (BPC1–7) that are broadly expressed, and they can potentially bind more than 3000 Arabidopsis GAGA-repeat-containing genes. To date, BPCs...

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Autores principales: Simonini, Sara, Kater, Martin M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3967085/
https://www.ncbi.nlm.nih.gov/pubmed/24482368
http://dx.doi.org/10.1093/jxb/eru003
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author Simonini, Sara
Kater, Martin M.
author_facet Simonini, Sara
Kater, Martin M.
author_sort Simonini, Sara
collection PubMed
description The BASIC PENTACYSTEINE (BCP) family is a poorly characterized plant transcription factor family of GAGA BINDING PROTEINS. In Arabidopsis, there are seven members (BPC1–7) that are broadly expressed, and they can potentially bind more than 3000 Arabidopsis GAGA-repeat-containing genes. To date, BPCs are known to be direct regulators of the INNER NO OUTER (INO), SEEDSTICK (STK), and LEAFY COTYLEDON 2 (LEC2) genes. Because of the high functional redundancy, neither single knockout nor double bpc mutant combinations cause aberrant phenotypes. The bpc1-2 bpc2 bpc3 triple mutant shows several pleiotropic developmental defects, including enlargement of the inflorescence meristem and flowers with supernumerary floral organs. Here, we demonstrated through expression analysis and chromatin immunoprecipitation assays that this phenotype is probably due to deregulation of the expression of the SHOOTMERISTEMLESS (STM) and BREVIPEDICELLUS/KNAT1 (BP) genes, which are both direct targets of BPCs. Moreover, we assigned a role to BPCs in the fine regulation of the cytokinin content in the meristem, as both ISOPENTENYLTRANSFERASE 7 (IPT7) and ARABIDOPSIS RESPONSE REGULATOR 7 (ARR7) genes were shown to be overexpressed in the bpc1-2 bpc2 bpc3 triple mutant.
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spelling pubmed-39670852014-06-18 Class I BASIC PENTACYSTEINE factors regulate HOMEOBOX genes involved in meristem size maintenance Simonini, Sara Kater, Martin M. J Exp Bot Research Paper The BASIC PENTACYSTEINE (BCP) family is a poorly characterized plant transcription factor family of GAGA BINDING PROTEINS. In Arabidopsis, there are seven members (BPC1–7) that are broadly expressed, and they can potentially bind more than 3000 Arabidopsis GAGA-repeat-containing genes. To date, BPCs are known to be direct regulators of the INNER NO OUTER (INO), SEEDSTICK (STK), and LEAFY COTYLEDON 2 (LEC2) genes. Because of the high functional redundancy, neither single knockout nor double bpc mutant combinations cause aberrant phenotypes. The bpc1-2 bpc2 bpc3 triple mutant shows several pleiotropic developmental defects, including enlargement of the inflorescence meristem and flowers with supernumerary floral organs. Here, we demonstrated through expression analysis and chromatin immunoprecipitation assays that this phenotype is probably due to deregulation of the expression of the SHOOTMERISTEMLESS (STM) and BREVIPEDICELLUS/KNAT1 (BP) genes, which are both direct targets of BPCs. Moreover, we assigned a role to BPCs in the fine regulation of the cytokinin content in the meristem, as both ISOPENTENYLTRANSFERASE 7 (IPT7) and ARABIDOPSIS RESPONSE REGULATOR 7 (ARR7) genes were shown to be overexpressed in the bpc1-2 bpc2 bpc3 triple mutant. Oxford University Press 2014-04 2014-01-30 /pmc/articles/PMC3967085/ /pubmed/24482368 http://dx.doi.org/10.1093/jxb/eru003 Text en © The Author 2014. Published by Oxford University Press on behalf of the Society for Experimental Biology. http://creativecommons.org/licenses/by/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Paper
Simonini, Sara
Kater, Martin M.
Class I BASIC PENTACYSTEINE factors regulate HOMEOBOX genes involved in meristem size maintenance
title Class I BASIC PENTACYSTEINE factors regulate HOMEOBOX genes involved in meristem size maintenance
title_full Class I BASIC PENTACYSTEINE factors regulate HOMEOBOX genes involved in meristem size maintenance
title_fullStr Class I BASIC PENTACYSTEINE factors regulate HOMEOBOX genes involved in meristem size maintenance
title_full_unstemmed Class I BASIC PENTACYSTEINE factors regulate HOMEOBOX genes involved in meristem size maintenance
title_short Class I BASIC PENTACYSTEINE factors regulate HOMEOBOX genes involved in meristem size maintenance
title_sort class i basic pentacysteine factors regulate homeobox genes involved in meristem size maintenance
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3967085/
https://www.ncbi.nlm.nih.gov/pubmed/24482368
http://dx.doi.org/10.1093/jxb/eru003
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