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Identification of new adventitious rooting mutants amongst suppressors of the Arabidopsis thaliana superroot2 mutation
The plant hormone auxin plays a central role in adventitious rooting and is routinely used with many economically important, vegetatively propagated plant species to promote adventitious root initiation and development on cuttings. Nevertheless the molecular mechanisms through which it acts are only...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3967091/ https://www.ncbi.nlm.nih.gov/pubmed/24596172 http://dx.doi.org/10.1093/jxb/eru026 |
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author | Pacurar, Daniel Ioan Pacurar, Monica Lacramioara Bussell, John Desmond Schwambach, Joseli Pop, Tiberia Ioana Kowalczyk, Mariusz Gutierrez, Laurent Cavel, Emilie Chaabouni, Salma Ljung, Karin Fett-Neto, Arthur Germano Pamfil, Doru Bellini, Catherine |
author_facet | Pacurar, Daniel Ioan Pacurar, Monica Lacramioara Bussell, John Desmond Schwambach, Joseli Pop, Tiberia Ioana Kowalczyk, Mariusz Gutierrez, Laurent Cavel, Emilie Chaabouni, Salma Ljung, Karin Fett-Neto, Arthur Germano Pamfil, Doru Bellini, Catherine |
author_sort | Pacurar, Daniel Ioan |
collection | PubMed |
description | The plant hormone auxin plays a central role in adventitious rooting and is routinely used with many economically important, vegetatively propagated plant species to promote adventitious root initiation and development on cuttings. Nevertheless the molecular mechanisms through which it acts are only starting to emerge. The Arabidopsis superroot2-1 (sur2-1) mutant overproduces auxin and, as a consequence, develops excessive adventitious roots in the hypocotyl. In order to increase the knowledge of adventitious rooting and of auxin signalling pathways and crosstalk, this study performed a screen for suppressors of superroot2-1 phenotype. These suppressors provide a new resource for discovery of genetic players involved in auxin signalling pathways or at the crosstalk of auxin and other hormones or environmental signals. This study reports the identification and characterization of 26 sur2-1 suppressor mutants, several of which were identified as mutations in candidate genes involved in either auxin biosynthesis or signalling. In addition to confirming the role of auxin as a central regulator of adventitious rooting, superroot2 suppressors indicated possible crosstalk with ethylene signalling in this process. |
format | Online Article Text |
id | pubmed-3967091 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-39670912014-06-18 Identification of new adventitious rooting mutants amongst suppressors of the Arabidopsis thaliana superroot2 mutation Pacurar, Daniel Ioan Pacurar, Monica Lacramioara Bussell, John Desmond Schwambach, Joseli Pop, Tiberia Ioana Kowalczyk, Mariusz Gutierrez, Laurent Cavel, Emilie Chaabouni, Salma Ljung, Karin Fett-Neto, Arthur Germano Pamfil, Doru Bellini, Catherine J Exp Bot Research Paper The plant hormone auxin plays a central role in adventitious rooting and is routinely used with many economically important, vegetatively propagated plant species to promote adventitious root initiation and development on cuttings. Nevertheless the molecular mechanisms through which it acts are only starting to emerge. The Arabidopsis superroot2-1 (sur2-1) mutant overproduces auxin and, as a consequence, develops excessive adventitious roots in the hypocotyl. In order to increase the knowledge of adventitious rooting and of auxin signalling pathways and crosstalk, this study performed a screen for suppressors of superroot2-1 phenotype. These suppressors provide a new resource for discovery of genetic players involved in auxin signalling pathways or at the crosstalk of auxin and other hormones or environmental signals. This study reports the identification and characterization of 26 sur2-1 suppressor mutants, several of which were identified as mutations in candidate genes involved in either auxin biosynthesis or signalling. In addition to confirming the role of auxin as a central regulator of adventitious rooting, superroot2 suppressors indicated possible crosstalk with ethylene signalling in this process. Oxford University Press 2014-04 2014-03-04 /pmc/articles/PMC3967091/ /pubmed/24596172 http://dx.doi.org/10.1093/jxb/eru026 Text en © The Author 2014. Published by Oxford University Press on behalf of the Society for Experimental Biology. http://creativecommons.org/licenses/by/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Paper Pacurar, Daniel Ioan Pacurar, Monica Lacramioara Bussell, John Desmond Schwambach, Joseli Pop, Tiberia Ioana Kowalczyk, Mariusz Gutierrez, Laurent Cavel, Emilie Chaabouni, Salma Ljung, Karin Fett-Neto, Arthur Germano Pamfil, Doru Bellini, Catherine Identification of new adventitious rooting mutants amongst suppressors of the Arabidopsis thaliana superroot2 mutation |
title | Identification of new adventitious rooting mutants amongst suppressors of the Arabidopsis thaliana superroot2 mutation |
title_full | Identification of new adventitious rooting mutants amongst suppressors of the Arabidopsis thaliana superroot2 mutation |
title_fullStr | Identification of new adventitious rooting mutants amongst suppressors of the Arabidopsis thaliana superroot2 mutation |
title_full_unstemmed | Identification of new adventitious rooting mutants amongst suppressors of the Arabidopsis thaliana superroot2 mutation |
title_short | Identification of new adventitious rooting mutants amongst suppressors of the Arabidopsis thaliana superroot2 mutation |
title_sort | identification of new adventitious rooting mutants amongst suppressors of the arabidopsis thaliana superroot2 mutation |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3967091/ https://www.ncbi.nlm.nih.gov/pubmed/24596172 http://dx.doi.org/10.1093/jxb/eru026 |
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