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Propolis Modulates Fibronectin Expression in the Matrix of Thermal Injury

The aim of the study was to assess the propolis effect on fibronectin metabolism in the course of burn wounds healing process. A model of burn wound healing of pig skin was applied. The amount of the released glycoprotein was assessed by a surface plasmon resonance. The profile of extracted fibronec...

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Autores principales: Olczyk, Pawel, Komosinska-Vassev, Katarzyna, Wisowski, Grzegorz, Mencner, Lukasz, Stojko, Jerzy, Kozma, Ewa M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3967494/
https://www.ncbi.nlm.nih.gov/pubmed/24738072
http://dx.doi.org/10.1155/2014/748101
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author Olczyk, Pawel
Komosinska-Vassev, Katarzyna
Wisowski, Grzegorz
Mencner, Lukasz
Stojko, Jerzy
Kozma, Ewa M.
author_facet Olczyk, Pawel
Komosinska-Vassev, Katarzyna
Wisowski, Grzegorz
Mencner, Lukasz
Stojko, Jerzy
Kozma, Ewa M.
author_sort Olczyk, Pawel
collection PubMed
description The aim of the study was to assess the propolis effect on fibronectin metabolism in the course of burn wounds healing process. A model of burn wound healing of pig skin was applied. The amount of the released glycoprotein was assessed by a surface plasmon resonance. The profile of extracted fibronectin components was also assessed by an electrophoresis in polyacrylamide gel, with a subsequent immunodetection by Western Blotting. Propolis burn treatment decreased the release of fibronectin components from healing wounds in relation to damages treated with silver sulfadiazine. The main reason of decreased extraction of fibronectin components from wounds treated with propolis was a substantial decrease of degradation product release of the mentioned glycoprotein, which was observed particularly from the 3rd to 5th day of the repair. Wounds treatment with propolis demonstrated, especially in relation to damages treated with silver sulfadiazine, the decreased release of synthesized fibronectin molecules. The obtained results suggest that propolis modifies fibronectin metabolism in the course of wound healing process. The influence of propolis is reflected in prevention of fibronectin biosynthesis as well as its degradation in the wound area. The above-mentioned metabolic changes may decrease the risk of complications in the repair wounds process.
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spelling pubmed-39674942014-04-15 Propolis Modulates Fibronectin Expression in the Matrix of Thermal Injury Olczyk, Pawel Komosinska-Vassev, Katarzyna Wisowski, Grzegorz Mencner, Lukasz Stojko, Jerzy Kozma, Ewa M. Biomed Res Int Research Article The aim of the study was to assess the propolis effect on fibronectin metabolism in the course of burn wounds healing process. A model of burn wound healing of pig skin was applied. The amount of the released glycoprotein was assessed by a surface plasmon resonance. The profile of extracted fibronectin components was also assessed by an electrophoresis in polyacrylamide gel, with a subsequent immunodetection by Western Blotting. Propolis burn treatment decreased the release of fibronectin components from healing wounds in relation to damages treated with silver sulfadiazine. The main reason of decreased extraction of fibronectin components from wounds treated with propolis was a substantial decrease of degradation product release of the mentioned glycoprotein, which was observed particularly from the 3rd to 5th day of the repair. Wounds treatment with propolis demonstrated, especially in relation to damages treated with silver sulfadiazine, the decreased release of synthesized fibronectin molecules. The obtained results suggest that propolis modifies fibronectin metabolism in the course of wound healing process. The influence of propolis is reflected in prevention of fibronectin biosynthesis as well as its degradation in the wound area. The above-mentioned metabolic changes may decrease the risk of complications in the repair wounds process. Hindawi Publishing Corporation 2014 2014-03-11 /pmc/articles/PMC3967494/ /pubmed/24738072 http://dx.doi.org/10.1155/2014/748101 Text en Copyright © 2014 Pawel Olczyk et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Olczyk, Pawel
Komosinska-Vassev, Katarzyna
Wisowski, Grzegorz
Mencner, Lukasz
Stojko, Jerzy
Kozma, Ewa M.
Propolis Modulates Fibronectin Expression in the Matrix of Thermal Injury
title Propolis Modulates Fibronectin Expression in the Matrix of Thermal Injury
title_full Propolis Modulates Fibronectin Expression in the Matrix of Thermal Injury
title_fullStr Propolis Modulates Fibronectin Expression in the Matrix of Thermal Injury
title_full_unstemmed Propolis Modulates Fibronectin Expression in the Matrix of Thermal Injury
title_short Propolis Modulates Fibronectin Expression in the Matrix of Thermal Injury
title_sort propolis modulates fibronectin expression in the matrix of thermal injury
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3967494/
https://www.ncbi.nlm.nih.gov/pubmed/24738072
http://dx.doi.org/10.1155/2014/748101
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