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An Improved Experimental Model of Hemorrhoids in Rats: Evaluation of Antihemorrhoidal Activity of an Herbal Formulation

Objective. To improve the existing experimental model of croton oil-induced hemorrhoids in rats by using Evans Blue (EB) dye extravasation technique. Further, an herbal formulation (Pilex) was evaluated for its antihemorrhoidal activity in this model. Methods. Two sets of experiments were carried ou...

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Autores principales: Azeemuddin, Mohammed, Viswanatha, Gollapalle Lakshminarayanashastry, Rafiq, Mohamed, Thippeswamy, Agadi HireMath, Baig, Mirza Rizwan, Kavya, Kethaganahalli Jayaramaiah, Patki, Pralhad Sadashiv, Shyam, Ramakrishnan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3967498/
https://www.ncbi.nlm.nih.gov/pubmed/25006493
http://dx.doi.org/10.1155/2014/530931
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author Azeemuddin, Mohammed
Viswanatha, Gollapalle Lakshminarayanashastry
Rafiq, Mohamed
Thippeswamy, Agadi HireMath
Baig, Mirza Rizwan
Kavya, Kethaganahalli Jayaramaiah
Patki, Pralhad Sadashiv
Shyam, Ramakrishnan
author_facet Azeemuddin, Mohammed
Viswanatha, Gollapalle Lakshminarayanashastry
Rafiq, Mohamed
Thippeswamy, Agadi HireMath
Baig, Mirza Rizwan
Kavya, Kethaganahalli Jayaramaiah
Patki, Pralhad Sadashiv
Shyam, Ramakrishnan
author_sort Azeemuddin, Mohammed
collection PubMed
description Objective. To improve the existing experimental model of croton oil-induced hemorrhoids in rats by using Evans Blue (EB) dye extravasation technique. Further, an herbal formulation (Pilex) was evaluated for its antihemorrhoidal activity in this model. Methods. Two sets of experiments were carried out: first to improve the experimental model and to validate the same using Pilex and second to evaluate the effect of Pilex on cytoarchitecture of rectoanal tissue in croton oil-induced hemorrhoids. In both sets, hemorrhoids were induced to all the animals, except normal controls, by applying croton oil via rectoanal region and the effect of Pilex ointment (PO), Pilex granules (PG), and combination of PG and PO was evaluated. In the first set, extravasation of EB dye, TNF-α, IL-6, and rectoanal coefficient (RAC) was determined. In the second set, severity of score, RAC, and histopathology were evaluated. Results. The elevated levels of TNF-α, IL-6, and extravasations of EB dye were decreased with the Pilex treatment. The cytoarchitecture of rectoanal portion of the animals treated with Pilex was near to normal. Conclusion. The improved experimental model of hemorrhoid is useful in quantifying the inflammatory exudates and extent of inflammation. In this improved experimental model Pilex showed antihemorrhoidal activity, which further validates its clinical usage.
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spelling pubmed-39674982014-07-08 An Improved Experimental Model of Hemorrhoids in Rats: Evaluation of Antihemorrhoidal Activity of an Herbal Formulation Azeemuddin, Mohammed Viswanatha, Gollapalle Lakshminarayanashastry Rafiq, Mohamed Thippeswamy, Agadi HireMath Baig, Mirza Rizwan Kavya, Kethaganahalli Jayaramaiah Patki, Pralhad Sadashiv Shyam, Ramakrishnan ISRN Pharmacol Research Article Objective. To improve the existing experimental model of croton oil-induced hemorrhoids in rats by using Evans Blue (EB) dye extravasation technique. Further, an herbal formulation (Pilex) was evaluated for its antihemorrhoidal activity in this model. Methods. Two sets of experiments were carried out: first to improve the experimental model and to validate the same using Pilex and second to evaluate the effect of Pilex on cytoarchitecture of rectoanal tissue in croton oil-induced hemorrhoids. In both sets, hemorrhoids were induced to all the animals, except normal controls, by applying croton oil via rectoanal region and the effect of Pilex ointment (PO), Pilex granules (PG), and combination of PG and PO was evaluated. In the first set, extravasation of EB dye, TNF-α, IL-6, and rectoanal coefficient (RAC) was determined. In the second set, severity of score, RAC, and histopathology were evaluated. Results. The elevated levels of TNF-α, IL-6, and extravasations of EB dye were decreased with the Pilex treatment. The cytoarchitecture of rectoanal portion of the animals treated with Pilex was near to normal. Conclusion. The improved experimental model of hemorrhoid is useful in quantifying the inflammatory exudates and extent of inflammation. In this improved experimental model Pilex showed antihemorrhoidal activity, which further validates its clinical usage. Hindawi Publishing Corporation 2014-03-11 /pmc/articles/PMC3967498/ /pubmed/25006493 http://dx.doi.org/10.1155/2014/530931 Text en Copyright © 2014 Mohammed Azeemuddin et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Azeemuddin, Mohammed
Viswanatha, Gollapalle Lakshminarayanashastry
Rafiq, Mohamed
Thippeswamy, Agadi HireMath
Baig, Mirza Rizwan
Kavya, Kethaganahalli Jayaramaiah
Patki, Pralhad Sadashiv
Shyam, Ramakrishnan
An Improved Experimental Model of Hemorrhoids in Rats: Evaluation of Antihemorrhoidal Activity of an Herbal Formulation
title An Improved Experimental Model of Hemorrhoids in Rats: Evaluation of Antihemorrhoidal Activity of an Herbal Formulation
title_full An Improved Experimental Model of Hemorrhoids in Rats: Evaluation of Antihemorrhoidal Activity of an Herbal Formulation
title_fullStr An Improved Experimental Model of Hemorrhoids in Rats: Evaluation of Antihemorrhoidal Activity of an Herbal Formulation
title_full_unstemmed An Improved Experimental Model of Hemorrhoids in Rats: Evaluation of Antihemorrhoidal Activity of an Herbal Formulation
title_short An Improved Experimental Model of Hemorrhoids in Rats: Evaluation of Antihemorrhoidal Activity of an Herbal Formulation
title_sort improved experimental model of hemorrhoids in rats: evaluation of antihemorrhoidal activity of an herbal formulation
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3967498/
https://www.ncbi.nlm.nih.gov/pubmed/25006493
http://dx.doi.org/10.1155/2014/530931
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