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Distinct immunologic effects of different intravenous iron preparations on monocytes

BACKGROUND: Iron deficiency contributes to anaemia in patients with chronic kidney disease. I.v. iron is therefore widely used for anaemia treatment, although it may induce oxidative stress and activate monocytes. Different i.v. iron preparations are available, but interestingly their substance-spec...

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Autores principales: Fell, Lisa H., Zawada, Adam M., Rogacev, Kyrill S., Seiler, Sarah, Fliser, Danilo, Heine, Gunnar H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3967833/
https://www.ncbi.nlm.nih.gov/pubmed/24523357
http://dx.doi.org/10.1093/ndt/gft524
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author Fell, Lisa H.
Zawada, Adam M.
Rogacev, Kyrill S.
Seiler, Sarah
Fliser, Danilo
Heine, Gunnar H.
author_facet Fell, Lisa H.
Zawada, Adam M.
Rogacev, Kyrill S.
Seiler, Sarah
Fliser, Danilo
Heine, Gunnar H.
author_sort Fell, Lisa H.
collection PubMed
description BACKGROUND: Iron deficiency contributes to anaemia in patients with chronic kidney disease. I.v. iron is therefore widely used for anaemia treatment, although it may induce oxidative stress and activate monocytes. Different i.v. iron preparations are available, but interestingly their substance-specific immunologic effects are poorly studied. METHODS: We analysed the effect of iron sucrose, ferric carboxymaltose, iron isomaltoside 1000, low-molecular-weight iron dextran and ferumoxytol on classical, intermediate and nonclassical monocyte biology. We therefore stimulated in vitro mature monocytes and haematopoietic CD34(+) stem cells during their differentiation into monocytes with different concentrations (0.133, 0.266, 0.533 mg/mL) of i.v. iron preparations. Alterations of monocyte subset distribution, expression of surface markers (CD86, CCR5, CX(3)CR1), as well as production of pro-inflammatory cytokines (TNF-α, IL-1β) and reactive oxygen species were measured using flow cytometry. Additionally, we analysed phagocytosis and antigen presentation capacity. RESULTS: We found specific immunologic effects after stimulation with iron sucrose which were not induced by the other iron preparations. Iron sucrose activated monocyte subsets leading to significantly increased CD86 expression. Simultaneously CD16 and CX(3)CR1 expression and monocytic phagocytosis capacity were decreased. Additionally, differentiation of monocytes from haematopoietic CD34(+) stem cells was almost completely abolished after stimulation with iron sucrose. CONCLUSIONS: Our findings demonstrate that specific immunologic effects of distinct i.v. iron preparations exist. The clinical relevance of these findings requires further investigation.
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spelling pubmed-39678332014-03-27 Distinct immunologic effects of different intravenous iron preparations on monocytes Fell, Lisa H. Zawada, Adam M. Rogacev, Kyrill S. Seiler, Sarah Fliser, Danilo Heine, Gunnar H. Nephrol Dial Transplant Original Articles BACKGROUND: Iron deficiency contributes to anaemia in patients with chronic kidney disease. I.v. iron is therefore widely used for anaemia treatment, although it may induce oxidative stress and activate monocytes. Different i.v. iron preparations are available, but interestingly their substance-specific immunologic effects are poorly studied. METHODS: We analysed the effect of iron sucrose, ferric carboxymaltose, iron isomaltoside 1000, low-molecular-weight iron dextran and ferumoxytol on classical, intermediate and nonclassical monocyte biology. We therefore stimulated in vitro mature monocytes and haematopoietic CD34(+) stem cells during their differentiation into monocytes with different concentrations (0.133, 0.266, 0.533 mg/mL) of i.v. iron preparations. Alterations of monocyte subset distribution, expression of surface markers (CD86, CCR5, CX(3)CR1), as well as production of pro-inflammatory cytokines (TNF-α, IL-1β) and reactive oxygen species were measured using flow cytometry. Additionally, we analysed phagocytosis and antigen presentation capacity. RESULTS: We found specific immunologic effects after stimulation with iron sucrose which were not induced by the other iron preparations. Iron sucrose activated monocyte subsets leading to significantly increased CD86 expression. Simultaneously CD16 and CX(3)CR1 expression and monocytic phagocytosis capacity were decreased. Additionally, differentiation of monocytes from haematopoietic CD34(+) stem cells was almost completely abolished after stimulation with iron sucrose. CONCLUSIONS: Our findings demonstrate that specific immunologic effects of distinct i.v. iron preparations exist. The clinical relevance of these findings requires further investigation. Oxford University Press 2014-04 2014-02-11 /pmc/articles/PMC3967833/ /pubmed/24523357 http://dx.doi.org/10.1093/ndt/gft524 Text en © The Author 2014. Published by Oxford University Press on behalf of ERA-EDTA. http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Original Articles
Fell, Lisa H.
Zawada, Adam M.
Rogacev, Kyrill S.
Seiler, Sarah
Fliser, Danilo
Heine, Gunnar H.
Distinct immunologic effects of different intravenous iron preparations on monocytes
title Distinct immunologic effects of different intravenous iron preparations on monocytes
title_full Distinct immunologic effects of different intravenous iron preparations on monocytes
title_fullStr Distinct immunologic effects of different intravenous iron preparations on monocytes
title_full_unstemmed Distinct immunologic effects of different intravenous iron preparations on monocytes
title_short Distinct immunologic effects of different intravenous iron preparations on monocytes
title_sort distinct immunologic effects of different intravenous iron preparations on monocytes
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3967833/
https://www.ncbi.nlm.nih.gov/pubmed/24523357
http://dx.doi.org/10.1093/ndt/gft524
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