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Integrating De Novo Transcriptome Assembly and Cloning to Obtain Chicken Ovocleidin-17 Full-Length cDNA

Efficiently obtaining full-length cDNA for a target gene is the key step for functional studies and probing genetic variations. However, almost all sequenced domestic animal genomes are not ‘finished’. Many functionally important genes are located in these gapped regions. It can be difficult to obta...

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Detalles Bibliográficos
Autores principales: Zhang, Quan, Liu, Long, Zhu, Feng, Ning, ZhongHua, Hincke, Maxwell T., Yang, Ning, Hou, ZhuoCheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3968166/
https://www.ncbi.nlm.nih.gov/pubmed/24676480
http://dx.doi.org/10.1371/journal.pone.0093452