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Physiological and photosynthetic characteristics of indica Hang2 expressing the sugarcane PEPC gene
Phosphoenolpyruvate carboxylase (PEPC) is known to play a key role in the initial fixation of CO(2) in C4 photosynthesis. The PEPC gene from sugarcane (a C4 plant) was introduced into indica rice (Hang2), a process mediated by Agrobacterium tumefaciens. Integration patterns and copy numbers of the g...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Netherlands
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3968443/ https://www.ncbi.nlm.nih.gov/pubmed/24469712 http://dx.doi.org/10.1007/s11033-014-3070-4 |
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author | Lian, Ling Wang, Xiaowei Zhu, Yongsheng He, Wei Cai, Qiuhua Xie, Huaan Zhang, Muqing Zhang, Jianfu |
author_facet | Lian, Ling Wang, Xiaowei Zhu, Yongsheng He, Wei Cai, Qiuhua Xie, Huaan Zhang, Muqing Zhang, Jianfu |
author_sort | Lian, Ling |
collection | PubMed |
description | Phosphoenolpyruvate carboxylase (PEPC) is known to play a key role in the initial fixation of CO(2) in C4 photosynthesis. The PEPC gene from sugarcane (a C4 plant) was introduced into indica rice (Hang2), a process mediated by Agrobacterium tumefaciens. Integration patterns and copy numbers of the gene was confirmed by DNA blot analysis. RT-PCR and western blotting results showed that the PEPC gene was expressed at both the mRNA and protein levels in the transgenic lines. Real-time PCR results indicated that expression of the sugarcane PEPC gene occurred mostly in green tissues and changed under high temperature and drought stress. All transgenic lines showed higher PEPC enzyme activities compared to the untransformed controls, with the highest activity (11.1 times higher than the controls) being observed in the transgenic line, T34. The transgenic lines also exhibited higher photosynthetic rates. The highest photosynthetic rate was observed in the transgenic line, T54 (22.3 μmol m(−2) s(−1); 24.6 % higher than that in non-transgenic plants) under high-temperature conditions. Furthermore, the filled grain and total grain numbers for transgenic lines were higher than those for non-transgenic plants, but the grain filling (%) and 1,000-grain weights of all transgenic lines remained unchanged. We concluded that over-expression of the PEPC gene from sugarcane in indica rice (Hang2) resulted in higher PEPC enzyme activities and higher photosynthesis rates under high-temperature conditions. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s11033-014-3070-4) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-3968443 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Springer Netherlands |
record_format | MEDLINE/PubMed |
spelling | pubmed-39684432014-03-28 Physiological and photosynthetic characteristics of indica Hang2 expressing the sugarcane PEPC gene Lian, Ling Wang, Xiaowei Zhu, Yongsheng He, Wei Cai, Qiuhua Xie, Huaan Zhang, Muqing Zhang, Jianfu Mol Biol Rep Article Phosphoenolpyruvate carboxylase (PEPC) is known to play a key role in the initial fixation of CO(2) in C4 photosynthesis. The PEPC gene from sugarcane (a C4 plant) was introduced into indica rice (Hang2), a process mediated by Agrobacterium tumefaciens. Integration patterns and copy numbers of the gene was confirmed by DNA blot analysis. RT-PCR and western blotting results showed that the PEPC gene was expressed at both the mRNA and protein levels in the transgenic lines. Real-time PCR results indicated that expression of the sugarcane PEPC gene occurred mostly in green tissues and changed under high temperature and drought stress. All transgenic lines showed higher PEPC enzyme activities compared to the untransformed controls, with the highest activity (11.1 times higher than the controls) being observed in the transgenic line, T34. The transgenic lines also exhibited higher photosynthetic rates. The highest photosynthetic rate was observed in the transgenic line, T54 (22.3 μmol m(−2) s(−1); 24.6 % higher than that in non-transgenic plants) under high-temperature conditions. Furthermore, the filled grain and total grain numbers for transgenic lines were higher than those for non-transgenic plants, but the grain filling (%) and 1,000-grain weights of all transgenic lines remained unchanged. We concluded that over-expression of the PEPC gene from sugarcane in indica rice (Hang2) resulted in higher PEPC enzyme activities and higher photosynthesis rates under high-temperature conditions. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s11033-014-3070-4) contains supplementary material, which is available to authorized users. Springer Netherlands 2014-01-29 2014 /pmc/articles/PMC3968443/ /pubmed/24469712 http://dx.doi.org/10.1007/s11033-014-3070-4 Text en © The Author(s) 2014 https://creativecommons.org/licenses/by/2.0/ Open AccessThis article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited. |
spellingShingle | Article Lian, Ling Wang, Xiaowei Zhu, Yongsheng He, Wei Cai, Qiuhua Xie, Huaan Zhang, Muqing Zhang, Jianfu Physiological and photosynthetic characteristics of indica Hang2 expressing the sugarcane PEPC gene |
title | Physiological and photosynthetic characteristics of indica Hang2 expressing the sugarcane PEPC gene |
title_full | Physiological and photosynthetic characteristics of indica Hang2 expressing the sugarcane PEPC gene |
title_fullStr | Physiological and photosynthetic characteristics of indica Hang2 expressing the sugarcane PEPC gene |
title_full_unstemmed | Physiological and photosynthetic characteristics of indica Hang2 expressing the sugarcane PEPC gene |
title_short | Physiological and photosynthetic characteristics of indica Hang2 expressing the sugarcane PEPC gene |
title_sort | physiological and photosynthetic characteristics of indica hang2 expressing the sugarcane pepc gene |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3968443/ https://www.ncbi.nlm.nih.gov/pubmed/24469712 http://dx.doi.org/10.1007/s11033-014-3070-4 |
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