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Cofactor analogue-induced chemical reactivation of endonuclease activity in a DNA cleavage/methylation deficient TspGWI N(473)A variant in the NPPY motif

We reported previously that TspGWI, a prototype enzyme of a new Thermus sp. family of restriction endonucleases-methyltransferases (REases-MTases), undergoes the novel phenomenon of sinefungin (SIN)-caused specificity transition. Here we investigated mutant TspGWI N(473)A, containing a single amino...

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Detalles Bibliográficos
Autores principales:	Zylicz-Stachula, Agnieszka, Jeżewska-Frąckowiak, Joanna, Skowron, Piotr M.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Springer Netherlands 2014
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3968444/ https://www.ncbi.nlm.nih.gov/pubmed/24442320 http://dx.doi.org/10.1007/s11033-014-3085-x

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