Exclusive formation of monovalent quantum dot imaging probes by steric exclusion

Precise control over interfacial chemistry between nanoparticles and other materials remains a significant challenge limiting the broad application of nanotechnology in biology. To address this challenge, we use “Steric Exclusion” to completely convert commercial quantum dots (QDs) into monovalent i...

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Autores principales: Farlow, Justin, Seo, Daeha, Broaders, Kyle E., Taylor, Marcus, Gartner, Zev J., Jun, Young-wook
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2013
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3968776/
https://www.ncbi.nlm.nih.gov/pubmed/24122039
http://dx.doi.org/10.1038/nmeth.2682
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author Farlow, Justin
Seo, Daeha
Broaders, Kyle E.
Taylor, Marcus
Gartner, Zev J.
Jun, Young-wook
author_facet Farlow, Justin
Seo, Daeha
Broaders, Kyle E.
Taylor, Marcus
Gartner, Zev J.
Jun, Young-wook
author_sort Farlow, Justin
collection PubMed
description Precise control over interfacial chemistry between nanoparticles and other materials remains a significant challenge limiting the broad application of nanotechnology in biology. To address this challenge, we use “Steric Exclusion” to completely convert commercial quantum dots (QDs) into monovalent imaging probes by wrapping the QD with a functionalized oligonucleotide. We demonstrate the utility of these QDs as modular and non-perturbing imaging probes by tracking individual Notch receptors on live cells.
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spelling pubmed-39687762014-06-01 Exclusive formation of monovalent quantum dot imaging probes by steric exclusion Farlow, Justin Seo, Daeha Broaders, Kyle E. Taylor, Marcus Gartner, Zev J. Jun, Young-wook Nat Methods Article Precise control over interfacial chemistry between nanoparticles and other materials remains a significant challenge limiting the broad application of nanotechnology in biology. To address this challenge, we use “Steric Exclusion” to completely convert commercial quantum dots (QDs) into monovalent imaging probes by wrapping the QD with a functionalized oligonucleotide. We demonstrate the utility of these QDs as modular and non-perturbing imaging probes by tracking individual Notch receptors on live cells. 2013-10-13 2013-12 /pmc/articles/PMC3968776/ /pubmed/24122039 http://dx.doi.org/10.1038/nmeth.2682 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Farlow, Justin
Seo, Daeha
Broaders, Kyle E.
Taylor, Marcus
Gartner, Zev J.
Jun, Young-wook
Exclusive formation of monovalent quantum dot imaging probes by steric exclusion
title Exclusive formation of monovalent quantum dot imaging probes by steric exclusion
title_full Exclusive formation of monovalent quantum dot imaging probes by steric exclusion
title_fullStr Exclusive formation of monovalent quantum dot imaging probes by steric exclusion
title_full_unstemmed Exclusive formation of monovalent quantum dot imaging probes by steric exclusion
title_short Exclusive formation of monovalent quantum dot imaging probes by steric exclusion
title_sort exclusive formation of monovalent quantum dot imaging probes by steric exclusion
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3968776/
https://www.ncbi.nlm.nih.gov/pubmed/24122039
http://dx.doi.org/10.1038/nmeth.2682
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