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Functional characterisation of bovine TLR5 indicates species-specific recognition of flagellin()
Mammalian toll-like receptor 5 (TLR5) senses flagellin of several bacterial species and has been described to activate the innate immune system. To assess the role of bovine TLR5 (boTLR5) in the cattle system, we cloned and successfully expressed boTLR5 in human embryonic kidney (HEK) 293 cells, as...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier Scientific
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3969226/ https://www.ncbi.nlm.nih.gov/pubmed/24461722 http://dx.doi.org/10.1016/j.vetimm.2013.12.006 |
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author | Metcalfe, Hannah J. La Ragione, Roberto M. Smith, David G.E. Werling, Dirk |
author_facet | Metcalfe, Hannah J. La Ragione, Roberto M. Smith, David G.E. Werling, Dirk |
author_sort | Metcalfe, Hannah J. |
collection | PubMed |
description | Mammalian toll-like receptor 5 (TLR5) senses flagellin of several bacterial species and has been described to activate the innate immune system. To assess the role of bovine TLR5 (boTLR5) in the cattle system, we cloned and successfully expressed boTLR5 in human embryonic kidney (HEK) 293 cells, as indicated by quantitative PCR and confocal microscopy. However, in contrast to huTLR5-transfected cells, exposure of boTLR5-transfected cells to flagellin neither activated nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) nor CXCL8 production. Subsequent comparison of the flagellin response induced in human and bovine primary macrophages revealed that flagellin did not lead to phosphorylation of major signalling molecules. Furthermore, the CXCL8 and TNFα response of primary bovine macrophages stimulated with flagellin was very low compared to that observed in human primary macrophages. Our results indicate that cattle express a functional TLR5 albeit with different flagellin sensing qualities compared to human TLR5. However, boTLR5 seemed to play a different role in the bovine system compared to the human system in recognizing flagellin, and other potentially intracellular expressed receptors may play a more important role in the bovine system to detect flagellin. |
format | Online Article Text |
id | pubmed-3969226 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Elsevier Scientific |
record_format | MEDLINE/PubMed |
spelling | pubmed-39692262014-03-31 Functional characterisation of bovine TLR5 indicates species-specific recognition of flagellin() Metcalfe, Hannah J. La Ragione, Roberto M. Smith, David G.E. Werling, Dirk Vet Immunol Immunopathol Short Communication Mammalian toll-like receptor 5 (TLR5) senses flagellin of several bacterial species and has been described to activate the innate immune system. To assess the role of bovine TLR5 (boTLR5) in the cattle system, we cloned and successfully expressed boTLR5 in human embryonic kidney (HEK) 293 cells, as indicated by quantitative PCR and confocal microscopy. However, in contrast to huTLR5-transfected cells, exposure of boTLR5-transfected cells to flagellin neither activated nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) nor CXCL8 production. Subsequent comparison of the flagellin response induced in human and bovine primary macrophages revealed that flagellin did not lead to phosphorylation of major signalling molecules. Furthermore, the CXCL8 and TNFα response of primary bovine macrophages stimulated with flagellin was very low compared to that observed in human primary macrophages. Our results indicate that cattle express a functional TLR5 albeit with different flagellin sensing qualities compared to human TLR5. However, boTLR5 seemed to play a different role in the bovine system compared to the human system in recognizing flagellin, and other potentially intracellular expressed receptors may play a more important role in the bovine system to detect flagellin. Elsevier Scientific 2014-02-15 /pmc/articles/PMC3969226/ /pubmed/24461722 http://dx.doi.org/10.1016/j.vetimm.2013.12.006 Text en © 2014 The Authors http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-No Derivative Works License, which permits non-commercial use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Short Communication Metcalfe, Hannah J. La Ragione, Roberto M. Smith, David G.E. Werling, Dirk Functional characterisation of bovine TLR5 indicates species-specific recognition of flagellin() |
title | Functional characterisation of bovine TLR5 indicates species-specific recognition of flagellin() |
title_full | Functional characterisation of bovine TLR5 indicates species-specific recognition of flagellin() |
title_fullStr | Functional characterisation of bovine TLR5 indicates species-specific recognition of flagellin() |
title_full_unstemmed | Functional characterisation of bovine TLR5 indicates species-specific recognition of flagellin() |
title_short | Functional characterisation of bovine TLR5 indicates species-specific recognition of flagellin() |
title_sort | functional characterisation of bovine tlr5 indicates species-specific recognition of flagellin() |
topic | Short Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3969226/ https://www.ncbi.nlm.nih.gov/pubmed/24461722 http://dx.doi.org/10.1016/j.vetimm.2013.12.006 |
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