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Arabidopsis Transcriptome Analysis Reveals Key Roles of Melatonin in Plant Defense Systems

Melatonin is a ubiquitous molecule and exists across kingdoms including plant species. Studies on melatonin in plants have mainly focused on its physiological influence on growth and development, and on its biosynthesis. Much less attention has been drawn to its affect on genome-wide gene expression...

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Autores principales: Weeda, Sarah, Zhang, Na, Zhao, Xiaolei, Ndip, Grace, Guo, Yangdong, Buck, Gregory A., Fu, Conggui, Ren, Shuxin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3969325/
https://www.ncbi.nlm.nih.gov/pubmed/24682084
http://dx.doi.org/10.1371/journal.pone.0093462
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author Weeda, Sarah
Zhang, Na
Zhao, Xiaolei
Ndip, Grace
Guo, Yangdong
Buck, Gregory A.
Fu, Conggui
Ren, Shuxin
author_facet Weeda, Sarah
Zhang, Na
Zhao, Xiaolei
Ndip, Grace
Guo, Yangdong
Buck, Gregory A.
Fu, Conggui
Ren, Shuxin
author_sort Weeda, Sarah
collection PubMed
description Melatonin is a ubiquitous molecule and exists across kingdoms including plant species. Studies on melatonin in plants have mainly focused on its physiological influence on growth and development, and on its biosynthesis. Much less attention has been drawn to its affect on genome-wide gene expression. To comprehensively investigate the role(s) of melatonin at the genomics level, we utilized mRNA-seq technology to analyze Arabidopsis plants subjected to a 16-hour 100 pM (low) and 1 mM (high) melatonin treatment. The expression profiles were analyzed to identify differentially expressed genes. 100 pM melatonin treatment significantly affected the expression of only 81 genes with 51 down-regulated and 30 up-regulated. However, 1 mM melatonin significantly altered 1308 genes with 566 up-regulated and 742 down-regulated. Not all genes altered by low melatonin were affected by high melatonin, indicating different roles of melatonin in regulation of plant growth and development under low and high concentrations. Furthermore, a large number of genes altered by melatonin were involved in plant stress defense. Transcript levels for many stress receptors, kinases, and stress-associated calcium signals were up-regulated. The majority of transcription factors identified were also involved in plant stress defense. Additionally, most identified genes in ABA, ET, SA and JA pathways were up-regulated, while genes pertaining to auxin responses and signaling, peroxidases, and those associated with cell wall synthesis and modifications were mostly down-regulated. Our results indicate critical roles of melatonin in plant defense against various environmental stresses, and provide a framework for functional analysis of genes in melatonin-mediated signaling pathways.
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spelling pubmed-39693252014-04-01 Arabidopsis Transcriptome Analysis Reveals Key Roles of Melatonin in Plant Defense Systems Weeda, Sarah Zhang, Na Zhao, Xiaolei Ndip, Grace Guo, Yangdong Buck, Gregory A. Fu, Conggui Ren, Shuxin PLoS One Research Article Melatonin is a ubiquitous molecule and exists across kingdoms including plant species. Studies on melatonin in plants have mainly focused on its physiological influence on growth and development, and on its biosynthesis. Much less attention has been drawn to its affect on genome-wide gene expression. To comprehensively investigate the role(s) of melatonin at the genomics level, we utilized mRNA-seq technology to analyze Arabidopsis plants subjected to a 16-hour 100 pM (low) and 1 mM (high) melatonin treatment. The expression profiles were analyzed to identify differentially expressed genes. 100 pM melatonin treatment significantly affected the expression of only 81 genes with 51 down-regulated and 30 up-regulated. However, 1 mM melatonin significantly altered 1308 genes with 566 up-regulated and 742 down-regulated. Not all genes altered by low melatonin were affected by high melatonin, indicating different roles of melatonin in regulation of plant growth and development under low and high concentrations. Furthermore, a large number of genes altered by melatonin were involved in plant stress defense. Transcript levels for many stress receptors, kinases, and stress-associated calcium signals were up-regulated. The majority of transcription factors identified were also involved in plant stress defense. Additionally, most identified genes in ABA, ET, SA and JA pathways were up-regulated, while genes pertaining to auxin responses and signaling, peroxidases, and those associated with cell wall synthesis and modifications were mostly down-regulated. Our results indicate critical roles of melatonin in plant defense against various environmental stresses, and provide a framework for functional analysis of genes in melatonin-mediated signaling pathways. Public Library of Science 2014-03-28 /pmc/articles/PMC3969325/ /pubmed/24682084 http://dx.doi.org/10.1371/journal.pone.0093462 Text en © 2014 Weeda et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Weeda, Sarah
Zhang, Na
Zhao, Xiaolei
Ndip, Grace
Guo, Yangdong
Buck, Gregory A.
Fu, Conggui
Ren, Shuxin
Arabidopsis Transcriptome Analysis Reveals Key Roles of Melatonin in Plant Defense Systems
title Arabidopsis Transcriptome Analysis Reveals Key Roles of Melatonin in Plant Defense Systems
title_full Arabidopsis Transcriptome Analysis Reveals Key Roles of Melatonin in Plant Defense Systems
title_fullStr Arabidopsis Transcriptome Analysis Reveals Key Roles of Melatonin in Plant Defense Systems
title_full_unstemmed Arabidopsis Transcriptome Analysis Reveals Key Roles of Melatonin in Plant Defense Systems
title_short Arabidopsis Transcriptome Analysis Reveals Key Roles of Melatonin in Plant Defense Systems
title_sort arabidopsis transcriptome analysis reveals key roles of melatonin in plant defense systems
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3969325/
https://www.ncbi.nlm.nih.gov/pubmed/24682084
http://dx.doi.org/10.1371/journal.pone.0093462
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