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Potential Regulatory Role of MicroRNAs in the Development of Bovine Gastrointestinal Tract during Early Life

This study aimed to investigate the potential regulatory role of miRNAs in the development of gastrointestinal tract (GIT) during the early life of dairy calves. Rumen and small intestinal (mid-jejunum and ileum) tissue samples were collected from newborn (30 min after birth; n = 3), 7-day-old (n = ...

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Autores principales: Liang, Guanxiang, Malmuthuge, Nilusha, McFadden, Thomas B., Bao, Hua, Griebel, Philip J., Stothard, Paul, Guan, Le Luo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3969364/
https://www.ncbi.nlm.nih.gov/pubmed/24682221
http://dx.doi.org/10.1371/journal.pone.0092592
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author Liang, Guanxiang
Malmuthuge, Nilusha
McFadden, Thomas B.
Bao, Hua
Griebel, Philip J.
Stothard, Paul
Guan, Le Luo
author_facet Liang, Guanxiang
Malmuthuge, Nilusha
McFadden, Thomas B.
Bao, Hua
Griebel, Philip J.
Stothard, Paul
Guan, Le Luo
author_sort Liang, Guanxiang
collection PubMed
description This study aimed to investigate the potential regulatory role of miRNAs in the development of gastrointestinal tract (GIT) during the early life of dairy calves. Rumen and small intestinal (mid-jejunum and ileum) tissue samples were collected from newborn (30 min after birth; n = 3), 7-day-old (n = 6), 21-day-old (n = 6), and 42-day-old (n = 6) dairy calves. The miRNA profiling was performed using Illumina RNA-sequencing and the temporal and regional differentially expressed miRNAs were further validated using qRT-PCR. Analysis of 16S rRNA gene copy numbers was used to quantify total bacteria, Bifidobacterium and Lactobacillus species. The expression of miR-143 was abundant in all three gut regions, at all time points and it targets genes involved primarily in the proliferation of connective tissue cells and muscle cells, suggesting a role in regulating rapid tissue development during the early life of calves. The expression of miR-146, miR-191, miR-33, miR-7, miR-99/100, miR-486, miR-145, miR-196 and miR-211 displayed significant temporal differences (FDR <0.05), while miR-192/215, miR-194, miR-196, miR-205 and miR-31 revealed significant regional differences (FDR <0.05). The expression levels of miR-15/16, miR-29 and miR-196 were positively correlated with the copy numbers of 16S rRNA gene of Bifidobacterium or Lactobacillus species or both (P<0.05). Functional analysis using Ingenuity Pathway Analysis identified the above mentioned differentially expressed miRNAs as potential regulators of gut tissue cell proliferation and differentiation. The bacterial density-associated miRNAs were identified as modulators of the development of lymphoid tissues (miR-196), maturation of dendritic cells (miR-29) and development of immune cells (miR-15/16). The present study revealed temporal and regional changes in miRNA expression and a correlation between miRNA expression and microbial population in the GIT during the early life, which provides further evidence for another mechanism by which host-microbial interactions play a role in regulating gut development.
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spelling pubmed-39693642014-04-01 Potential Regulatory Role of MicroRNAs in the Development of Bovine Gastrointestinal Tract during Early Life Liang, Guanxiang Malmuthuge, Nilusha McFadden, Thomas B. Bao, Hua Griebel, Philip J. Stothard, Paul Guan, Le Luo PLoS One Research Article This study aimed to investigate the potential regulatory role of miRNAs in the development of gastrointestinal tract (GIT) during the early life of dairy calves. Rumen and small intestinal (mid-jejunum and ileum) tissue samples were collected from newborn (30 min after birth; n = 3), 7-day-old (n = 6), 21-day-old (n = 6), and 42-day-old (n = 6) dairy calves. The miRNA profiling was performed using Illumina RNA-sequencing and the temporal and regional differentially expressed miRNAs were further validated using qRT-PCR. Analysis of 16S rRNA gene copy numbers was used to quantify total bacteria, Bifidobacterium and Lactobacillus species. The expression of miR-143 was abundant in all three gut regions, at all time points and it targets genes involved primarily in the proliferation of connective tissue cells and muscle cells, suggesting a role in regulating rapid tissue development during the early life of calves. The expression of miR-146, miR-191, miR-33, miR-7, miR-99/100, miR-486, miR-145, miR-196 and miR-211 displayed significant temporal differences (FDR <0.05), while miR-192/215, miR-194, miR-196, miR-205 and miR-31 revealed significant regional differences (FDR <0.05). The expression levels of miR-15/16, miR-29 and miR-196 were positively correlated with the copy numbers of 16S rRNA gene of Bifidobacterium or Lactobacillus species or both (P<0.05). Functional analysis using Ingenuity Pathway Analysis identified the above mentioned differentially expressed miRNAs as potential regulators of gut tissue cell proliferation and differentiation. The bacterial density-associated miRNAs were identified as modulators of the development of lymphoid tissues (miR-196), maturation of dendritic cells (miR-29) and development of immune cells (miR-15/16). The present study revealed temporal and regional changes in miRNA expression and a correlation between miRNA expression and microbial population in the GIT during the early life, which provides further evidence for another mechanism by which host-microbial interactions play a role in regulating gut development. Public Library of Science 2014-03-28 /pmc/articles/PMC3969364/ /pubmed/24682221 http://dx.doi.org/10.1371/journal.pone.0092592 Text en © 2014 Liang et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Liang, Guanxiang
Malmuthuge, Nilusha
McFadden, Thomas B.
Bao, Hua
Griebel, Philip J.
Stothard, Paul
Guan, Le Luo
Potential Regulatory Role of MicroRNAs in the Development of Bovine Gastrointestinal Tract during Early Life
title Potential Regulatory Role of MicroRNAs in the Development of Bovine Gastrointestinal Tract during Early Life
title_full Potential Regulatory Role of MicroRNAs in the Development of Bovine Gastrointestinal Tract during Early Life
title_fullStr Potential Regulatory Role of MicroRNAs in the Development of Bovine Gastrointestinal Tract during Early Life
title_full_unstemmed Potential Regulatory Role of MicroRNAs in the Development of Bovine Gastrointestinal Tract during Early Life
title_short Potential Regulatory Role of MicroRNAs in the Development of Bovine Gastrointestinal Tract during Early Life
title_sort potential regulatory role of micrornas in the development of bovine gastrointestinal tract during early life
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3969364/
https://www.ncbi.nlm.nih.gov/pubmed/24682221
http://dx.doi.org/10.1371/journal.pone.0092592
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