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Deciphering early events involved in hyperosmotic stress-induced programmed cell death in tobacco BY-2 cells

Hyperosmotic stresses represent one of the major constraints that adversely affect plants growth, development, and productivity. In this study, the focus was on early responses to hyperosmotic stress- (NaCl and sorbitol) induced reactive oxygen species (ROS) generation, cytosolic Ca(2+) concentratio...

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Autores principales: Monetti, Emanuela, Kadono, Takashi, Tran, Daniel, Azzarello, Elisa, Arbelet-Bonnin, Delphine, Biligui, Bernadette, Briand, Joël, Kawano, Tomonori, Mancuso, Stefano, Bouteau, François
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3969528/
https://www.ncbi.nlm.nih.gov/pubmed/24420571
http://dx.doi.org/10.1093/jxb/ert460
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author Monetti, Emanuela
Kadono, Takashi
Tran, Daniel
Azzarello, Elisa
Arbelet-Bonnin, Delphine
Biligui, Bernadette
Briand, Joël
Kawano, Tomonori
Mancuso, Stefano
Bouteau, François
author_facet Monetti, Emanuela
Kadono, Takashi
Tran, Daniel
Azzarello, Elisa
Arbelet-Bonnin, Delphine
Biligui, Bernadette
Briand, Joël
Kawano, Tomonori
Mancuso, Stefano
Bouteau, François
author_sort Monetti, Emanuela
collection PubMed
description Hyperosmotic stresses represent one of the major constraints that adversely affect plants growth, development, and productivity. In this study, the focus was on early responses to hyperosmotic stress- (NaCl and sorbitol) induced reactive oxygen species (ROS) generation, cytosolic Ca(2+) concentration ([Ca(2+)](cyt)) increase, ion fluxes, and mitochondrial potential variations, and on their links in pathways leading to programmed cell death (PCD). By using BY-2 tobacco cells, it was shown that both NaCl- and sorbitol-induced PCD seemed to be dependent on superoxide anion (O(2)·(–)) generation by NADPH-oxidase. In the case of NaCl, an early influx of sodium through non-selective cation channels participates in the development of PCD through mitochondrial dysfunction and NADPH-oxidase-dependent O(2)·(–) generation. This supports the hypothesis of different pathways in NaCl- and sorbitol-induced cell death. Surprisingly, other shared early responses, such as [Ca(2+)](cyt) increase and singlet oxygen production, do not seem to be involved in PCD.
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spelling pubmed-39695282014-06-18 Deciphering early events involved in hyperosmotic stress-induced programmed cell death in tobacco BY-2 cells Monetti, Emanuela Kadono, Takashi Tran, Daniel Azzarello, Elisa Arbelet-Bonnin, Delphine Biligui, Bernadette Briand, Joël Kawano, Tomonori Mancuso, Stefano Bouteau, François J Exp Bot Research Paper Hyperosmotic stresses represent one of the major constraints that adversely affect plants growth, development, and productivity. In this study, the focus was on early responses to hyperosmotic stress- (NaCl and sorbitol) induced reactive oxygen species (ROS) generation, cytosolic Ca(2+) concentration ([Ca(2+)](cyt)) increase, ion fluxes, and mitochondrial potential variations, and on their links in pathways leading to programmed cell death (PCD). By using BY-2 tobacco cells, it was shown that both NaCl- and sorbitol-induced PCD seemed to be dependent on superoxide anion (O(2)·(–)) generation by NADPH-oxidase. In the case of NaCl, an early influx of sodium through non-selective cation channels participates in the development of PCD through mitochondrial dysfunction and NADPH-oxidase-dependent O(2)·(–) generation. This supports the hypothesis of different pathways in NaCl- and sorbitol-induced cell death. Surprisingly, other shared early responses, such as [Ca(2+)](cyt) increase and singlet oxygen production, do not seem to be involved in PCD. Oxford University Press 2014-03 2014-01-13 /pmc/articles/PMC3969528/ /pubmed/24420571 http://dx.doi.org/10.1093/jxb/ert460 Text en © The Author 2014. Published by Oxford University Press on behalf of the Society for Experimental Biology. http://creativecommons.org/licenses/by/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Paper
Monetti, Emanuela
Kadono, Takashi
Tran, Daniel
Azzarello, Elisa
Arbelet-Bonnin, Delphine
Biligui, Bernadette
Briand, Joël
Kawano, Tomonori
Mancuso, Stefano
Bouteau, François
Deciphering early events involved in hyperosmotic stress-induced programmed cell death in tobacco BY-2 cells
title Deciphering early events involved in hyperosmotic stress-induced programmed cell death in tobacco BY-2 cells
title_full Deciphering early events involved in hyperosmotic stress-induced programmed cell death in tobacco BY-2 cells
title_fullStr Deciphering early events involved in hyperosmotic stress-induced programmed cell death in tobacco BY-2 cells
title_full_unstemmed Deciphering early events involved in hyperosmotic stress-induced programmed cell death in tobacco BY-2 cells
title_short Deciphering early events involved in hyperosmotic stress-induced programmed cell death in tobacco BY-2 cells
title_sort deciphering early events involved in hyperosmotic stress-induced programmed cell death in tobacco by-2 cells
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3969528/
https://www.ncbi.nlm.nih.gov/pubmed/24420571
http://dx.doi.org/10.1093/jxb/ert460
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