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Correlation of cell surface marker expression with African swine fever virus infection()
The expression of surface markers on African swine fever virus (ASFV) infected cells was evaluated to assess their involvement in infection. Previous findings indicated CD163 expression was correlated with ASFV susceptibility. However, in this study the expression of porcine CD163 on cell lines did...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier Scientific Pub. Co
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3969584/ https://www.ncbi.nlm.nih.gov/pubmed/24398227 http://dx.doi.org/10.1016/j.vetmic.2013.12.001 |
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author | Lithgow, Pamela Takamatsu, Haru Werling, Dirk Dixon, Linda Chapman, Dave |
author_facet | Lithgow, Pamela Takamatsu, Haru Werling, Dirk Dixon, Linda Chapman, Dave |
author_sort | Lithgow, Pamela |
collection | PubMed |
description | The expression of surface markers on African swine fever virus (ASFV) infected cells was evaluated to assess their involvement in infection. Previous findings indicated CD163 expression was correlated with ASFV susceptibility. However, in this study the expression of porcine CD163 on cell lines did not increase the infection rate of these cells indicating other factors are likely to be important in determining susceptibility to infection. On adherent porcine bone marrow (pBM) cells the expression of CD45 was strongly correlated with infection. CD163 and CD203a expression correlated at intermediate levels with infection, indicating cells expressing these markers could become infected but were not preferentially infected by the virus. Most of the cells expressing MHCII were infected, indicating that they may be preferentially infected although expression of MHCII was not essential for infection and a large percentage of the infected cells were MHCII negative. CD16 showed a marked decrease in expression following infection and significantly lower levels of infected cells were shown to express CD16. Altogether these results suggest CD163 may be involved in ASFV infection but it may not be essential; the results also highlight the importance of other cell markers which requiring further investigation. |
format | Online Article Text |
id | pubmed-3969584 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Elsevier Scientific Pub. Co |
record_format | MEDLINE/PubMed |
spelling | pubmed-39695842014-03-31 Correlation of cell surface marker expression with African swine fever virus infection() Lithgow, Pamela Takamatsu, Haru Werling, Dirk Dixon, Linda Chapman, Dave Vet Microbiol Short Communication The expression of surface markers on African swine fever virus (ASFV) infected cells was evaluated to assess their involvement in infection. Previous findings indicated CD163 expression was correlated with ASFV susceptibility. However, in this study the expression of porcine CD163 on cell lines did not increase the infection rate of these cells indicating other factors are likely to be important in determining susceptibility to infection. On adherent porcine bone marrow (pBM) cells the expression of CD45 was strongly correlated with infection. CD163 and CD203a expression correlated at intermediate levels with infection, indicating cells expressing these markers could become infected but were not preferentially infected by the virus. Most of the cells expressing MHCII were infected, indicating that they may be preferentially infected although expression of MHCII was not essential for infection and a large percentage of the infected cells were MHCII negative. CD16 showed a marked decrease in expression following infection and significantly lower levels of infected cells were shown to express CD16. Altogether these results suggest CD163 may be involved in ASFV infection but it may not be essential; the results also highlight the importance of other cell markers which requiring further investigation. Elsevier Scientific Pub. Co 2014-01-31 /pmc/articles/PMC3969584/ /pubmed/24398227 http://dx.doi.org/10.1016/j.vetmic.2013.12.001 Text en © 2013 The Authors http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Short Communication Lithgow, Pamela Takamatsu, Haru Werling, Dirk Dixon, Linda Chapman, Dave Correlation of cell surface marker expression with African swine fever virus infection() |
title | Correlation of cell surface marker expression with African swine fever virus infection() |
title_full | Correlation of cell surface marker expression with African swine fever virus infection() |
title_fullStr | Correlation of cell surface marker expression with African swine fever virus infection() |
title_full_unstemmed | Correlation of cell surface marker expression with African swine fever virus infection() |
title_short | Correlation of cell surface marker expression with African swine fever virus infection() |
title_sort | correlation of cell surface marker expression with african swine fever virus infection() |
topic | Short Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3969584/ https://www.ncbi.nlm.nih.gov/pubmed/24398227 http://dx.doi.org/10.1016/j.vetmic.2013.12.001 |
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