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Infection in a rat model reactivates attenuated virulence after long-term axenic culture of Acanthamoeba spp

Prolonged culturing of many microorganisms leads to the loss of virulence and a reduction of their infective capacity. However, little is known about the changes in the pathogenic strains of Acanthamoeba after long culture periods. Our study evaluated the effect of prolonged culturing on the invasiv...

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Detalles Bibliográficos
Autores principales: Veríssimo, Carolina De Marco, Maschio, Vinícius José, Correa, Ana Paula Folmer, Brandelli, Adriano, Rott, Marilise Brittes
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Instituto Oswaldo Cruz 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3970641/
https://www.ncbi.nlm.nih.gov/pubmed/24271042
http://dx.doi.org/10.1590/0074-0276130099
Descripción
Sumario:Prolonged culturing of many microorganisms leads to the loss of virulence and a reduction of their infective capacity. However, little is known about the changes in the pathogenic strains of Acanthamoeba after long culture periods. Our study evaluated the effect of prolonged culturing on the invasiveness of different isolates of Acanthamoeba in an in vivo rat model. ATCC strains of Acanthamoeba, isolates from the environment and clinical cases were evaluated. The in vivo model was effective in establishing the infection and differentiating the pathogenicity of the isolates and re-isolates. The amoebae cultured in the laboratory for long periods were less virulent than those that were recently isolated, confirming the importance of passing Acanthamoeba strains in animal models.