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Infection in a rat model reactivates attenuated virulence after long-term axenic culture of Acanthamoeba spp

Prolonged culturing of many microorganisms leads to the loss of virulence and a reduction of their infective capacity. However, little is known about the changes in the pathogenic strains of Acanthamoeba after long culture periods. Our study evaluated the effect of prolonged culturing on the invasiv...

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Autores principales: Veríssimo, Carolina De Marco, Maschio, Vinícius José, Correa, Ana Paula Folmer, Brandelli, Adriano, Rott, Marilise Brittes
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Instituto Oswaldo Cruz 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3970641/
https://www.ncbi.nlm.nih.gov/pubmed/24271042
http://dx.doi.org/10.1590/0074-0276130099
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author Veríssimo, Carolina De Marco
Maschio, Vinícius José
Correa, Ana Paula Folmer
Brandelli, Adriano
Rott, Marilise Brittes
author_facet Veríssimo, Carolina De Marco
Maschio, Vinícius José
Correa, Ana Paula Folmer
Brandelli, Adriano
Rott, Marilise Brittes
author_sort Veríssimo, Carolina De Marco
collection PubMed
description Prolonged culturing of many microorganisms leads to the loss of virulence and a reduction of their infective capacity. However, little is known about the changes in the pathogenic strains of Acanthamoeba after long culture periods. Our study evaluated the effect of prolonged culturing on the invasiveness of different isolates of Acanthamoeba in an in vivo rat model. ATCC strains of Acanthamoeba, isolates from the environment and clinical cases were evaluated. The in vivo model was effective in establishing the infection and differentiating the pathogenicity of the isolates and re-isolates. The amoebae cultured in the laboratory for long periods were less virulent than those that were recently isolated, confirming the importance of passing Acanthamoeba strains in animal models.
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spelling pubmed-39706412014-05-21 Infection in a rat model reactivates attenuated virulence after long-term axenic culture of Acanthamoeba spp Veríssimo, Carolina De Marco Maschio, Vinícius José Correa, Ana Paula Folmer Brandelli, Adriano Rott, Marilise Brittes Mem Inst Oswaldo Cruz Articles Prolonged culturing of many microorganisms leads to the loss of virulence and a reduction of their infective capacity. However, little is known about the changes in the pathogenic strains of Acanthamoeba after long culture periods. Our study evaluated the effect of prolonged culturing on the invasiveness of different isolates of Acanthamoeba in an in vivo rat model. ATCC strains of Acanthamoeba, isolates from the environment and clinical cases were evaluated. The in vivo model was effective in establishing the infection and differentiating the pathogenicity of the isolates and re-isolates. The amoebae cultured in the laboratory for long periods were less virulent than those that were recently isolated, confirming the importance of passing Acanthamoeba strains in animal models. Instituto Oswaldo Cruz 2013-11 /pmc/articles/PMC3970641/ /pubmed/24271042 http://dx.doi.org/10.1590/0074-0276130099 Text en http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Articles
Veríssimo, Carolina De Marco
Maschio, Vinícius José
Correa, Ana Paula Folmer
Brandelli, Adriano
Rott, Marilise Brittes
Infection in a rat model reactivates attenuated virulence after long-term axenic culture of Acanthamoeba spp
title Infection in a rat model reactivates attenuated virulence after long-term axenic culture of Acanthamoeba spp
title_full Infection in a rat model reactivates attenuated virulence after long-term axenic culture of Acanthamoeba spp
title_fullStr Infection in a rat model reactivates attenuated virulence after long-term axenic culture of Acanthamoeba spp
title_full_unstemmed Infection in a rat model reactivates attenuated virulence after long-term axenic culture of Acanthamoeba spp
title_short Infection in a rat model reactivates attenuated virulence after long-term axenic culture of Acanthamoeba spp
title_sort infection in a rat model reactivates attenuated virulence after long-term axenic culture of acanthamoeba spp
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3970641/
https://www.ncbi.nlm.nih.gov/pubmed/24271042
http://dx.doi.org/10.1590/0074-0276130099
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