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Evaluation of a chemiluminescent enzyme-linked immunosorbent assay for the diagnosis of Trypanosoma cruzi infection in a nonendemic setting

The disappearance of lytic, protective antibodies (Abs) from the serum of patients with Chagas disease is accepted as a reliable indicator of parasitological cure. The efficiency of a chemiluminescent enzyme-linked immunosorbent assay based on a purified, trypomastigote-derived glycosylphosphatidyli...

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Detalles Bibliográficos
Autores principales: Izquierdo, Luis, Marques, Alexandre Ferreira, Gállego, Montserrat, Sanz, Sílvia, Tebar, Sílvia, Riera, Cristina, Quintó, Llorenç, Aldasoro, Edelweiss, Almeida, Igor C, Gascon, Joaquim
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Instituto Oswaldo Cruz 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3970649/
https://www.ncbi.nlm.nih.gov/pubmed/24271047
http://dx.doi.org/10.1590/0074-0276130112
Descripción
Sumario:The disappearance of lytic, protective antibodies (Abs) from the serum of patients with Chagas disease is accepted as a reliable indicator of parasitological cure. The efficiency of a chemiluminescent enzyme-linked immunosorbent assay based on a purified, trypomastigote-derived glycosylphosphatidylinositol-anchored mucin antigen for the serologic detection of lytic Abs against Trypanosoma cruzi was evaluated in a nonendemic setting using a panel of 92 positive and 58 negative human sera. The technique proved to be highly sensitive {100%; 95% confidence interval (CI) = 96-100} and specific (98.3%; 95% CI = 90.7-99.7), with a kappa score of 0.99. Therefore, this assay can be used to detect active T. cruzi infection and to monitor trypanosomicidal treatment.