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Effect of treatment with cyclophosphamide in low doses upon the onset of delayed type hypersensitivity in mice chronically infected with Trypanosoma cruzi: involvement of heart interstitial dendritic cells
Acute infection with Trypanosoma cruzi results in intense myocarditis, which progresses to a chronic, asymptomatic indeterminate form. The evolution toward this chronic cardiac form occurs in approximately 30% of all cases of T. cruzi infection. Suppression of delayed type hypersensitivity (DTH) has...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Instituto Oswaldo Cruz, Ministério da Saúde
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3970680/ https://www.ncbi.nlm.nih.gov/pubmed/24037190 http://dx.doi.org/10.1590/0074-0276108062013004 |
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author | Thé, Torriceli Souza Portella, Renata Siqueira Guerreiro, Marcos Lázaro Andrade, Sonia Gumes |
author_facet | Thé, Torriceli Souza Portella, Renata Siqueira Guerreiro, Marcos Lázaro Andrade, Sonia Gumes |
author_sort | Thé, Torriceli Souza |
collection | PubMed |
description | Acute infection with Trypanosoma cruzi results in intense myocarditis, which progresses to a chronic, asymptomatic indeterminate form. The evolution toward this chronic cardiac form occurs in approximately 30% of all cases of T. cruzi infection. Suppression of delayed type hypersensitivity (DTH) has been proposed as a potential explanation of the indeterminate form. We investigated the effect of cyclophosphamide (CYCL) treatment on the regulatory mechanism of DTH and the participation of heart interstitial dendritic cells (IDCs) in this process using BALB/c mice chronically infected with T. cruzi. One group was treated with CYCL (20 mg/kg body weight) for one month. A DTH skin test was performed by intradermal injection of T. cruzi antigen (3 mg/mL) in the hind-footpad and measured the skin thickness after 24 h, 48 h and 72 h. The skin test revealed increased thickness in antigen-injected footpads, which was more evident in the mice treated with CYCL than in those mice that did not receive treatment. The thickened regions were characterised by perivascular infiltrates and areas of necrosis. Intense lesions of the myocardium were present in three/16 cases and included large areas of necrosis. Morphometric evaluation of lymphocytes showed a predominance of TCD8 cells. Heart IDCs were immunolabelled with specific antibodies (CD11b and CD11c) and T. cruzi antigens were detected using a specific anti-T. cruzi antibody. Identification of T. cruzi antigens, sequestered in these cells using specific anti-T. cruzi antibodies was done, showing a significant increase in the number of these cells in treated mice. These results indicate that IDCs participate in the regulatory mechanisms of DTH response to T. cruzi infection. |
format | Online Article Text |
id | pubmed-3970680 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Instituto Oswaldo Cruz, Ministério da Saúde |
record_format | MEDLINE/PubMed |
spelling | pubmed-39706802014-05-21 Effect of treatment with cyclophosphamide in low doses upon the onset of delayed type hypersensitivity in mice chronically infected with Trypanosoma cruzi: involvement of heart interstitial dendritic cells Thé, Torriceli Souza Portella, Renata Siqueira Guerreiro, Marcos Lázaro Andrade, Sonia Gumes Mem Inst Oswaldo Cruz Articles Acute infection with Trypanosoma cruzi results in intense myocarditis, which progresses to a chronic, asymptomatic indeterminate form. The evolution toward this chronic cardiac form occurs in approximately 30% of all cases of T. cruzi infection. Suppression of delayed type hypersensitivity (DTH) has been proposed as a potential explanation of the indeterminate form. We investigated the effect of cyclophosphamide (CYCL) treatment on the regulatory mechanism of DTH and the participation of heart interstitial dendritic cells (IDCs) in this process using BALB/c mice chronically infected with T. cruzi. One group was treated with CYCL (20 mg/kg body weight) for one month. A DTH skin test was performed by intradermal injection of T. cruzi antigen (3 mg/mL) in the hind-footpad and measured the skin thickness after 24 h, 48 h and 72 h. The skin test revealed increased thickness in antigen-injected footpads, which was more evident in the mice treated with CYCL than in those mice that did not receive treatment. The thickened regions were characterised by perivascular infiltrates and areas of necrosis. Intense lesions of the myocardium were present in three/16 cases and included large areas of necrosis. Morphometric evaluation of lymphocytes showed a predominance of TCD8 cells. Heart IDCs were immunolabelled with specific antibodies (CD11b and CD11c) and T. cruzi antigens were detected using a specific anti-T. cruzi antibody. Identification of T. cruzi antigens, sequestered in these cells using specific anti-T. cruzi antibodies was done, showing a significant increase in the number of these cells in treated mice. These results indicate that IDCs participate in the regulatory mechanisms of DTH response to T. cruzi infection. Instituto Oswaldo Cruz, Ministério da Saúde 2013-09 /pmc/articles/PMC3970680/ /pubmed/24037190 http://dx.doi.org/10.1590/0074-0276108062013004 Text en http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Articles Thé, Torriceli Souza Portella, Renata Siqueira Guerreiro, Marcos Lázaro Andrade, Sonia Gumes Effect of treatment with cyclophosphamide in low doses upon the onset of delayed type hypersensitivity in mice chronically infected with Trypanosoma cruzi: involvement of heart interstitial dendritic cells |
title | Effect of treatment with cyclophosphamide in low doses
upon the onset of delayed type hypersensitivity in mice chronically infected
with Trypanosoma cruzi: involvement of heart interstitial
dendritic cells |
title_full | Effect of treatment with cyclophosphamide in low doses
upon the onset of delayed type hypersensitivity in mice chronically infected
with Trypanosoma cruzi: involvement of heart interstitial
dendritic cells |
title_fullStr | Effect of treatment with cyclophosphamide in low doses
upon the onset of delayed type hypersensitivity in mice chronically infected
with Trypanosoma cruzi: involvement of heart interstitial
dendritic cells |
title_full_unstemmed | Effect of treatment with cyclophosphamide in low doses
upon the onset of delayed type hypersensitivity in mice chronically infected
with Trypanosoma cruzi: involvement of heart interstitial
dendritic cells |
title_short | Effect of treatment with cyclophosphamide in low doses
upon the onset of delayed type hypersensitivity in mice chronically infected
with Trypanosoma cruzi: involvement of heart interstitial
dendritic cells |
title_sort | effect of treatment with cyclophosphamide in low doses
upon the onset of delayed type hypersensitivity in mice chronically infected
with trypanosoma cruzi: involvement of heart interstitial
dendritic cells |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3970680/ https://www.ncbi.nlm.nih.gov/pubmed/24037190 http://dx.doi.org/10.1590/0074-0276108062013004 |
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