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Standardisation of Western blotting to detect HTLV-1 antibodies synthesised in the central nervous system of HAM/TSP patients
Intrathecal synthesis of human T-lymphotropic virus type 1 (HTLV-1) antibodies (Abs) represents conclusive evidence of a specific immune response in the central nervous system of HTLV-1 associated myelopathy/tropical spastic paraparesis (HAM/TSP) patients. Western blotting (WB) for HTLV Abs in serum...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Instituto Oswaldo Cruz, Ministério da Saúde
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3970696/ https://www.ncbi.nlm.nih.gov/pubmed/24037195 http://dx.doi.org/10.1590/0074-0276108062013009 |
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author | Ribeiro, Luiz Claudio Pereira Gonçalves, Cassia Cristina Alves Slater, Carla Maria Sena Andrade de Carvalho, Silvia Maia Farias Puccioni-Sohler, Marzia |
author_facet | Ribeiro, Luiz Claudio Pereira Gonçalves, Cassia Cristina Alves Slater, Carla Maria Sena Andrade de Carvalho, Silvia Maia Farias Puccioni-Sohler, Marzia |
author_sort | Ribeiro, Luiz Claudio Pereira |
collection | PubMed |
description | Intrathecal synthesis of human T-lymphotropic virus type 1 (HTLV-1) antibodies (Abs) represents conclusive evidence of a specific immune response in the central nervous system of HTLV-1 associated myelopathy/tropical spastic paraparesis (HAM/TSP) patients. Western blotting (WB) for HTLV Abs in serum is a confirmatory test for HTLV-1 infection. The aim of this study was to standardise the Western blot to demonstrate the intrathecal pattern of Abs against HTLV-1 proteins in HAM/TSP patients. Paired cerebrospinal fluid (CSF) and serum samples were selected from 20 patients with definite HAM/TSP, 19 HTLV-1 seronegative patients and two HTLV-1 patients without definite HAM/TSP. The presence of reactive bands of greater intensity in the CSF compared to serum (or bands in only the CSF) indicated the intrathecal synthesis of anti-HTLV-1 Abs. All definite HAM/TSP patients presented with an intrathecal synthesis of anti-HTLV-1 Abs; these Abs were not detected in the control patients. The most frequent intrathecal targets of anti-HTLV-1 Abs were GD21, rgp46-I and p24 and, to a lesser extent, p19, p26, p28, p32, p36, p53 gp21 and gp46. The intrathecal immune response against env (GD21 and rgp46-I) and gag (p24) proteins represents the most important humoral pattern in HAM/TSP. This response may be used as a diagnostic marker, considering the frequent association of intrathecal anti-HTLV-1 Ab synthesis with HAM/TSP and the pathogenesis of this neurological disease. |
format | Online Article Text |
id | pubmed-3970696 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Instituto Oswaldo Cruz, Ministério da Saúde |
record_format | MEDLINE/PubMed |
spelling | pubmed-39706962014-05-21 Standardisation of Western blotting to detect HTLV-1 antibodies synthesised in the central nervous system of HAM/TSP patients Ribeiro, Luiz Claudio Pereira Gonçalves, Cassia Cristina Alves Slater, Carla Maria Sena Andrade de Carvalho, Silvia Maia Farias Puccioni-Sohler, Marzia Mem Inst Oswaldo Cruz Articles Intrathecal synthesis of human T-lymphotropic virus type 1 (HTLV-1) antibodies (Abs) represents conclusive evidence of a specific immune response in the central nervous system of HTLV-1 associated myelopathy/tropical spastic paraparesis (HAM/TSP) patients. Western blotting (WB) for HTLV Abs in serum is a confirmatory test for HTLV-1 infection. The aim of this study was to standardise the Western blot to demonstrate the intrathecal pattern of Abs against HTLV-1 proteins in HAM/TSP patients. Paired cerebrospinal fluid (CSF) and serum samples were selected from 20 patients with definite HAM/TSP, 19 HTLV-1 seronegative patients and two HTLV-1 patients without definite HAM/TSP. The presence of reactive bands of greater intensity in the CSF compared to serum (or bands in only the CSF) indicated the intrathecal synthesis of anti-HTLV-1 Abs. All definite HAM/TSP patients presented with an intrathecal synthesis of anti-HTLV-1 Abs; these Abs were not detected in the control patients. The most frequent intrathecal targets of anti-HTLV-1 Abs were GD21, rgp46-I and p24 and, to a lesser extent, p19, p26, p28, p32, p36, p53 gp21 and gp46. The intrathecal immune response against env (GD21 and rgp46-I) and gag (p24) proteins represents the most important humoral pattern in HAM/TSP. This response may be used as a diagnostic marker, considering the frequent association of intrathecal anti-HTLV-1 Ab synthesis with HAM/TSP and the pathogenesis of this neurological disease. Instituto Oswaldo Cruz, Ministério da Saúde 2013-09 /pmc/articles/PMC3970696/ /pubmed/24037195 http://dx.doi.org/10.1590/0074-0276108062013009 Text en http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Articles Ribeiro, Luiz Claudio Pereira Gonçalves, Cassia Cristina Alves Slater, Carla Maria Sena Andrade de Carvalho, Silvia Maia Farias Puccioni-Sohler, Marzia Standardisation of Western blotting to detect HTLV-1 antibodies synthesised in the central nervous system of HAM/TSP patients |
title | Standardisation of Western blotting to detect HTLV-1
antibodies synthesised in the central nervous system of HAM/TSP
patients |
title_full | Standardisation of Western blotting to detect HTLV-1
antibodies synthesised in the central nervous system of HAM/TSP
patients |
title_fullStr | Standardisation of Western blotting to detect HTLV-1
antibodies synthesised in the central nervous system of HAM/TSP
patients |
title_full_unstemmed | Standardisation of Western blotting to detect HTLV-1
antibodies synthesised in the central nervous system of HAM/TSP
patients |
title_short | Standardisation of Western blotting to detect HTLV-1
antibodies synthesised in the central nervous system of HAM/TSP
patients |
title_sort | standardisation of western blotting to detect htlv-1
antibodies synthesised in the central nervous system of ham/tsp
patients |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3970696/ https://www.ncbi.nlm.nih.gov/pubmed/24037195 http://dx.doi.org/10.1590/0074-0276108062013009 |
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