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Surface engineered antifouling optomagnetic SPIONs for bimodal targeted imaging of pancreatic cancer cells

Targeted imaging contrast agents for early pancreatic ductal adenocarcinoma diagnosis was developed using superparamagnetic iron oxide nanoparticles (SPIONs). For phase transfer of SPIONs, the hydrophobic SPIONs are first treated with tetrafluoroborate and then capped by bovine serum albumin (BSA) v...

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Autores principales: Wang, Xiaohui, Xing, Xiaohong, Zhang, Bingbo, Liu, Fengjun, Cheng, Yingsheng, Shi, Donglu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3970947/
https://www.ncbi.nlm.nih.gov/pubmed/24741308
http://dx.doi.org/10.2147/IJN.S58334
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author Wang, Xiaohui
Xing, Xiaohong
Zhang, Bingbo
Liu, Fengjun
Cheng, Yingsheng
Shi, Donglu
author_facet Wang, Xiaohui
Xing, Xiaohong
Zhang, Bingbo
Liu, Fengjun
Cheng, Yingsheng
Shi, Donglu
author_sort Wang, Xiaohui
collection PubMed
description Targeted imaging contrast agents for early pancreatic ductal adenocarcinoma diagnosis was developed using superparamagnetic iron oxide nanoparticles (SPIONs). For phase transfer of SPIONs, the hydrophobic SPIONs are first treated with tetrafluoroborate and then capped by bovine serum albumin (BSA) via ligand exchange. It was experimentally found that nitrosyl tetrafluoroborate pretreatment and proper structures of molecules are essential to the effective surface functionalization of SPIONs. Nonspecific binding was found to be significantly reduced by BSA surface functionalized hydrophobic SPIONs (BSA·SPIONs). The BSA·SPIONs were monodispersed with an average size of approximately 18.0 nm and stable in a wide pH range and various ionic strengths even after 7 days of storage. The longitudinal and transverse proton relaxation rate (r(1), r(2)) values of the BSA·SPIONs were determined to be 11.6 and 154.2 s(−1) per mM of Fe(3+) respectively. The r(2)/r(1) ratio of 13.3 ensured its application as the T(2)-weighted magnetic resonance imaging contrast agents. When conjugated with near-infrared fluorescent dye and monoclonal antibody, the (dye)BSA·SPION-monoclonal antibody bioconjugates showed excellent targeting capability with minimal nonspecific binding in the bimodal imaging of pancreatic cancer cells. The experimental approach is facile, environmentally benign, and straightforward, which presents great promise in early cancer diagnosis.
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spelling pubmed-39709472014-04-16 Surface engineered antifouling optomagnetic SPIONs for bimodal targeted imaging of pancreatic cancer cells Wang, Xiaohui Xing, Xiaohong Zhang, Bingbo Liu, Fengjun Cheng, Yingsheng Shi, Donglu Int J Nanomedicine Original Research Targeted imaging contrast agents for early pancreatic ductal adenocarcinoma diagnosis was developed using superparamagnetic iron oxide nanoparticles (SPIONs). For phase transfer of SPIONs, the hydrophobic SPIONs are first treated with tetrafluoroborate and then capped by bovine serum albumin (BSA) via ligand exchange. It was experimentally found that nitrosyl tetrafluoroborate pretreatment and proper structures of molecules are essential to the effective surface functionalization of SPIONs. Nonspecific binding was found to be significantly reduced by BSA surface functionalized hydrophobic SPIONs (BSA·SPIONs). The BSA·SPIONs were monodispersed with an average size of approximately 18.0 nm and stable in a wide pH range and various ionic strengths even after 7 days of storage. The longitudinal and transverse proton relaxation rate (r(1), r(2)) values of the BSA·SPIONs were determined to be 11.6 and 154.2 s(−1) per mM of Fe(3+) respectively. The r(2)/r(1) ratio of 13.3 ensured its application as the T(2)-weighted magnetic resonance imaging contrast agents. When conjugated with near-infrared fluorescent dye and monoclonal antibody, the (dye)BSA·SPION-monoclonal antibody bioconjugates showed excellent targeting capability with minimal nonspecific binding in the bimodal imaging of pancreatic cancer cells. The experimental approach is facile, environmentally benign, and straightforward, which presents great promise in early cancer diagnosis. Dove Medical Press 2014-03-27 /pmc/articles/PMC3970947/ /pubmed/24741308 http://dx.doi.org/10.2147/IJN.S58334 Text en © 2014 Wang et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.
spellingShingle Original Research
Wang, Xiaohui
Xing, Xiaohong
Zhang, Bingbo
Liu, Fengjun
Cheng, Yingsheng
Shi, Donglu
Surface engineered antifouling optomagnetic SPIONs for bimodal targeted imaging of pancreatic cancer cells
title Surface engineered antifouling optomagnetic SPIONs for bimodal targeted imaging of pancreatic cancer cells
title_full Surface engineered antifouling optomagnetic SPIONs for bimodal targeted imaging of pancreatic cancer cells
title_fullStr Surface engineered antifouling optomagnetic SPIONs for bimodal targeted imaging of pancreatic cancer cells
title_full_unstemmed Surface engineered antifouling optomagnetic SPIONs for bimodal targeted imaging of pancreatic cancer cells
title_short Surface engineered antifouling optomagnetic SPIONs for bimodal targeted imaging of pancreatic cancer cells
title_sort surface engineered antifouling optomagnetic spions for bimodal targeted imaging of pancreatic cancer cells
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3970947/
https://www.ncbi.nlm.nih.gov/pubmed/24741308
http://dx.doi.org/10.2147/IJN.S58334
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