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P. falciparum Isolate-Specific Distinct Patterns of Induced Apoptosis in Pulmonary and Brain Endothelial Cells
The factors implicated in the transition from uncomplicated to severe clinical malaria such as pulmonary oedema and cerebral malaria remain unclear. It is known that alterations in vascular integrity due to endothelial cell (EC) activation and death occur during severe malaria. In this study, we ass...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3970966/ https://www.ncbi.nlm.nih.gov/pubmed/24686750 http://dx.doi.org/10.1371/journal.pone.0090692 |
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author | N'Dilimabaka, Nadine Taoufiq, Zacharie Zougbédé, Sergine Bonnefoy, Serge Lorthiois, Audrey Couraud, Pierre Oliver Rebollo, Angelita Snounou, Georges Mazier, Dominique Moreno Sabater, Alicia |
author_facet | N'Dilimabaka, Nadine Taoufiq, Zacharie Zougbédé, Sergine Bonnefoy, Serge Lorthiois, Audrey Couraud, Pierre Oliver Rebollo, Angelita Snounou, Georges Mazier, Dominique Moreno Sabater, Alicia |
author_sort | N'Dilimabaka, Nadine |
collection | PubMed |
description | The factors implicated in the transition from uncomplicated to severe clinical malaria such as pulmonary oedema and cerebral malaria remain unclear. It is known that alterations in vascular integrity due to endothelial cell (EC) activation and death occur during severe malaria. In this study, we assessed the ability of different P. falciparum clinical isolates to induce apoptosis in ECs derived from human lung and brain. We observed that induction of EC apoptosis was sensitive to the environmental pH and required direct contact between the parasite and the cell, though it was not correlated to the ability of the parasite to cytoadhere. Moreover, the extent of induced apoptosis in the two EC types varied with the isolate. Analysis of parasite genes transcript led us to propose that the activation of different pathways, such as Plasmodium apoptosis–linked pathogenicity factors (PALPF), PALPF-2, PALPF-5 and PF11_0521, could be implied in EC death. These observations provide an experimental framework to decipher the molecular mechanism implicated in the genesis of severe malaria. |
format | Online Article Text |
id | pubmed-3970966 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-39709662014-04-04 P. falciparum Isolate-Specific Distinct Patterns of Induced Apoptosis in Pulmonary and Brain Endothelial Cells N'Dilimabaka, Nadine Taoufiq, Zacharie Zougbédé, Sergine Bonnefoy, Serge Lorthiois, Audrey Couraud, Pierre Oliver Rebollo, Angelita Snounou, Georges Mazier, Dominique Moreno Sabater, Alicia PLoS One Research Article The factors implicated in the transition from uncomplicated to severe clinical malaria such as pulmonary oedema and cerebral malaria remain unclear. It is known that alterations in vascular integrity due to endothelial cell (EC) activation and death occur during severe malaria. In this study, we assessed the ability of different P. falciparum clinical isolates to induce apoptosis in ECs derived from human lung and brain. We observed that induction of EC apoptosis was sensitive to the environmental pH and required direct contact between the parasite and the cell, though it was not correlated to the ability of the parasite to cytoadhere. Moreover, the extent of induced apoptosis in the two EC types varied with the isolate. Analysis of parasite genes transcript led us to propose that the activation of different pathways, such as Plasmodium apoptosis–linked pathogenicity factors (PALPF), PALPF-2, PALPF-5 and PF11_0521, could be implied in EC death. These observations provide an experimental framework to decipher the molecular mechanism implicated in the genesis of severe malaria. Public Library of Science 2014-03-31 /pmc/articles/PMC3970966/ /pubmed/24686750 http://dx.doi.org/10.1371/journal.pone.0090692 Text en © 2014 N'Dilimabaka et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article N'Dilimabaka, Nadine Taoufiq, Zacharie Zougbédé, Sergine Bonnefoy, Serge Lorthiois, Audrey Couraud, Pierre Oliver Rebollo, Angelita Snounou, Georges Mazier, Dominique Moreno Sabater, Alicia P. falciparum Isolate-Specific Distinct Patterns of Induced Apoptosis in Pulmonary and Brain Endothelial Cells |
title |
P. falciparum Isolate-Specific Distinct Patterns of Induced Apoptosis in Pulmonary and Brain Endothelial Cells |
title_full |
P. falciparum Isolate-Specific Distinct Patterns of Induced Apoptosis in Pulmonary and Brain Endothelial Cells |
title_fullStr |
P. falciparum Isolate-Specific Distinct Patterns of Induced Apoptosis in Pulmonary and Brain Endothelial Cells |
title_full_unstemmed |
P. falciparum Isolate-Specific Distinct Patterns of Induced Apoptosis in Pulmonary and Brain Endothelial Cells |
title_short |
P. falciparum Isolate-Specific Distinct Patterns of Induced Apoptosis in Pulmonary and Brain Endothelial Cells |
title_sort | p. falciparum isolate-specific distinct patterns of induced apoptosis in pulmonary and brain endothelial cells |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3970966/ https://www.ncbi.nlm.nih.gov/pubmed/24686750 http://dx.doi.org/10.1371/journal.pone.0090692 |
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