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Establishment and biological characterization of a dermal mesenchymal stem cells line from bovine
The DMSCs (dermal mesenchymal stem cells) are multipotent stem cells, which can differentiate in vitro into many cell types. Much work has been done on DMSCs from humans, mice, rabbits and other mammals, but the related literature has not been published about these cells in cattle. In this study, we...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Portland Press Ltd.
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3971452/ https://www.ncbi.nlm.nih.gov/pubmed/27919035 http://dx.doi.org/10.1042/BSR20130095 |
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author | Sun, Tingting Yu, Chao Gao, Yuhua Zhao, Chenqiong Hua, Jinlian Cai, Lianshun Guan, Weijun Ma, Yuehui |
author_facet | Sun, Tingting Yu, Chao Gao, Yuhua Zhao, Chenqiong Hua, Jinlian Cai, Lianshun Guan, Weijun Ma, Yuehui |
author_sort | Sun, Tingting |
collection | PubMed |
description | The DMSCs (dermal mesenchymal stem cells) are multipotent stem cells, which can differentiate in vitro into many cell types. Much work has been done on DMSCs from humans, mice, rabbits and other mammals, but the related literature has not been published about these cells in cattle. In this study, we isolated and established the DMSC lines from cattle, thereby initiating further research on these cells, such as growth kinetics, detection of special surface antigen and RT–PCR (reverse transcription–PCR) assays to identify the biological characterization of the cell line. Furthermore, the DMSCs are induced to differentiate into adipocytes, osteoblasts and neural cells in vitro. Our results suggest that DMSCs isolated from cattle possess similar biological characteristics with those from other species. Their multi-lineage differentiation capabilities herald a probable application model in tissue engineering and induced pluripotent stem cells. |
format | Online Article Text |
id | pubmed-3971452 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Portland Press Ltd. |
record_format | MEDLINE/PubMed |
spelling | pubmed-39714522014-04-16 Establishment and biological characterization of a dermal mesenchymal stem cells line from bovine Sun, Tingting Yu, Chao Gao, Yuhua Zhao, Chenqiong Hua, Jinlian Cai, Lianshun Guan, Weijun Ma, Yuehui Biosci Rep Original Paper The DMSCs (dermal mesenchymal stem cells) are multipotent stem cells, which can differentiate in vitro into many cell types. Much work has been done on DMSCs from humans, mice, rabbits and other mammals, but the related literature has not been published about these cells in cattle. In this study, we isolated and established the DMSC lines from cattle, thereby initiating further research on these cells, such as growth kinetics, detection of special surface antigen and RT–PCR (reverse transcription–PCR) assays to identify the biological characterization of the cell line. Furthermore, the DMSCs are induced to differentiate into adipocytes, osteoblasts and neural cells in vitro. Our results suggest that DMSCs isolated from cattle possess similar biological characteristics with those from other species. Their multi-lineage differentiation capabilities herald a probable application model in tissue engineering and induced pluripotent stem cells. Portland Press Ltd. 2014-03-31 /pmc/articles/PMC3971452/ /pubmed/27919035 http://dx.doi.org/10.1042/BSR20130095 Text en © 2014 The author(s) has paid for this article to be freely available under the terms of the Creative Commons Attribution Licence (CC-BY)(http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited. http://creativecommons.org/licenses/by/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Licence (CC-BY) (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Paper Sun, Tingting Yu, Chao Gao, Yuhua Zhao, Chenqiong Hua, Jinlian Cai, Lianshun Guan, Weijun Ma, Yuehui Establishment and biological characterization of a dermal mesenchymal stem cells line from bovine |
title | Establishment and biological characterization of a dermal mesenchymal stem cells line from bovine |
title_full | Establishment and biological characterization of a dermal mesenchymal stem cells line from bovine |
title_fullStr | Establishment and biological characterization of a dermal mesenchymal stem cells line from bovine |
title_full_unstemmed | Establishment and biological characterization of a dermal mesenchymal stem cells line from bovine |
title_short | Establishment and biological characterization of a dermal mesenchymal stem cells line from bovine |
title_sort | establishment and biological characterization of a dermal mesenchymal stem cells line from bovine |
topic | Original Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3971452/ https://www.ncbi.nlm.nih.gov/pubmed/27919035 http://dx.doi.org/10.1042/BSR20130095 |
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