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Establishment and biological characterization of a dermal mesenchymal stem cells line from bovine

The DMSCs (dermal mesenchymal stem cells) are multipotent stem cells, which can differentiate in vitro into many cell types. Much work has been done on DMSCs from humans, mice, rabbits and other mammals, but the related literature has not been published about these cells in cattle. In this study, we...

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Autores principales: Sun, Tingting, Yu, Chao, Gao, Yuhua, Zhao, Chenqiong, Hua, Jinlian, Cai, Lianshun, Guan, Weijun, Ma, Yuehui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Portland Press Ltd. 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3971452/
https://www.ncbi.nlm.nih.gov/pubmed/27919035
http://dx.doi.org/10.1042/BSR20130095
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author Sun, Tingting
Yu, Chao
Gao, Yuhua
Zhao, Chenqiong
Hua, Jinlian
Cai, Lianshun
Guan, Weijun
Ma, Yuehui
author_facet Sun, Tingting
Yu, Chao
Gao, Yuhua
Zhao, Chenqiong
Hua, Jinlian
Cai, Lianshun
Guan, Weijun
Ma, Yuehui
author_sort Sun, Tingting
collection PubMed
description The DMSCs (dermal mesenchymal stem cells) are multipotent stem cells, which can differentiate in vitro into many cell types. Much work has been done on DMSCs from humans, mice, rabbits and other mammals, but the related literature has not been published about these cells in cattle. In this study, we isolated and established the DMSC lines from cattle, thereby initiating further research on these cells, such as growth kinetics, detection of special surface antigen and RT–PCR (reverse transcription–PCR) assays to identify the biological characterization of the cell line. Furthermore, the DMSCs are induced to differentiate into adipocytes, osteoblasts and neural cells in vitro. Our results suggest that DMSCs isolated from cattle possess similar biological characteristics with those from other species. Their multi-lineage differentiation capabilities herald a probable application model in tissue engineering and induced pluripotent stem cells.
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spelling pubmed-39714522014-04-16 Establishment and biological characterization of a dermal mesenchymal stem cells line from bovine Sun, Tingting Yu, Chao Gao, Yuhua Zhao, Chenqiong Hua, Jinlian Cai, Lianshun Guan, Weijun Ma, Yuehui Biosci Rep Original Paper The DMSCs (dermal mesenchymal stem cells) are multipotent stem cells, which can differentiate in vitro into many cell types. Much work has been done on DMSCs from humans, mice, rabbits and other mammals, but the related literature has not been published about these cells in cattle. In this study, we isolated and established the DMSC lines from cattle, thereby initiating further research on these cells, such as growth kinetics, detection of special surface antigen and RT–PCR (reverse transcription–PCR) assays to identify the biological characterization of the cell line. Furthermore, the DMSCs are induced to differentiate into adipocytes, osteoblasts and neural cells in vitro. Our results suggest that DMSCs isolated from cattle possess similar biological characteristics with those from other species. Their multi-lineage differentiation capabilities herald a probable application model in tissue engineering and induced pluripotent stem cells. Portland Press Ltd. 2014-03-31 /pmc/articles/PMC3971452/ /pubmed/27919035 http://dx.doi.org/10.1042/BSR20130095 Text en © 2014 The author(s) has paid for this article to be freely available under the terms of the Creative Commons Attribution Licence (CC-BY)(http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited. http://creativecommons.org/licenses/by/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Licence (CC-BY) (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Paper
Sun, Tingting
Yu, Chao
Gao, Yuhua
Zhao, Chenqiong
Hua, Jinlian
Cai, Lianshun
Guan, Weijun
Ma, Yuehui
Establishment and biological characterization of a dermal mesenchymal stem cells line from bovine
title Establishment and biological characterization of a dermal mesenchymal stem cells line from bovine
title_full Establishment and biological characterization of a dermal mesenchymal stem cells line from bovine
title_fullStr Establishment and biological characterization of a dermal mesenchymal stem cells line from bovine
title_full_unstemmed Establishment and biological characterization of a dermal mesenchymal stem cells line from bovine
title_short Establishment and biological characterization of a dermal mesenchymal stem cells line from bovine
title_sort establishment and biological characterization of a dermal mesenchymal stem cells line from bovine
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3971452/
https://www.ncbi.nlm.nih.gov/pubmed/27919035
http://dx.doi.org/10.1042/BSR20130095
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