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Filter-Aided N-Glycan Separation (FANGS): A Convenient Sample Preparation Method for Mass Spectrometric N-Glycan Profiling

[Image: see text] We have developed a simple method for the release and isolation of glycoprotein N-glycans from whole-cell lysates using less than a million cells, for subsequent implementation with mass spectrometric analysis. Cellular protein extracts prepared using SDS solubilization were sequen...

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Detalles Bibliográficos
Autores principales:	Abdul Rahman, Salina, Bergström, Ed, Watson, Christopher J., Wilson, Katherine M., Ashford, David A., Thomas, Jerry R., Ungar, Daniel, Thomas-Oates, Jane E.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	American Chemical Society 2014
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3971760/ https://www.ncbi.nlm.nih.gov/pubmed/24450425 http://dx.doi.org/10.1021/pr401043r

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