Regulation of the Expression of the β-Lactam Antibiotic-Resistance Determinants in Methicillin-Resistant Staphylococcus aureus (MRSA)

[Image: see text] β-Lactam antibiotics have faced obsolescence with the emergence of methicillin-resistant Staphylococcus aureus (MRSA). A complex set of events ensues upon exposure of MRSA to these antibiotics, which culminates in proteolysis of BlaI or MecI, two gene repressors, and results in the...

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Autores principales: Blázquez, Blas, Llarrull, Leticia I., Luque-Ortega, Juan R., Alfonso, Carlos, Boggess, Bill, Mobashery, Shahriar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2014
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3971960/
https://www.ncbi.nlm.nih.gov/pubmed/24564530
http://dx.doi.org/10.1021/bi500074w
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author Blázquez, Blas
Llarrull, Leticia I.
Luque-Ortega, Juan R.
Alfonso, Carlos
Boggess, Bill
Mobashery, Shahriar
author_facet Blázquez, Blas
Llarrull, Leticia I.
Luque-Ortega, Juan R.
Alfonso, Carlos
Boggess, Bill
Mobashery, Shahriar
author_sort Blázquez, Blas
collection PubMed
description [Image: see text] β-Lactam antibiotics have faced obsolescence with the emergence of methicillin-resistant Staphylococcus aureus (MRSA). A complex set of events ensues upon exposure of MRSA to these antibiotics, which culminates in proteolysis of BlaI or MecI, two gene repressors, and results in the induction of resistance. We report studies on the mechanism of binding of these gene repressors to the operator regions by fluorescence anisotropy. Within the range of in vivo concentrations for BlaI and MecI, these proteins interact with their regulatory elements in a reversible manner, as both a monomer and a dimer.
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spelling pubmed-39719602015-02-24 Regulation of the Expression of the β-Lactam Antibiotic-Resistance Determinants in Methicillin-Resistant Staphylococcus aureus (MRSA) Blázquez, Blas Llarrull, Leticia I. Luque-Ortega, Juan R. Alfonso, Carlos Boggess, Bill Mobashery, Shahriar Biochemistry [Image: see text] β-Lactam antibiotics have faced obsolescence with the emergence of methicillin-resistant Staphylococcus aureus (MRSA). A complex set of events ensues upon exposure of MRSA to these antibiotics, which culminates in proteolysis of BlaI or MecI, two gene repressors, and results in the induction of resistance. We report studies on the mechanism of binding of these gene repressors to the operator regions by fluorescence anisotropy. Within the range of in vivo concentrations for BlaI and MecI, these proteins interact with their regulatory elements in a reversible manner, as both a monomer and a dimer. American Chemical Society 2014-02-24 2014-03-18 /pmc/articles/PMC3971960/ /pubmed/24564530 http://dx.doi.org/10.1021/bi500074w Text en Copyright © 2014 American Chemical Society
spellingShingle Blázquez, Blas
Llarrull, Leticia I.
Luque-Ortega, Juan R.
Alfonso, Carlos
Boggess, Bill
Mobashery, Shahriar
Regulation of the Expression of the β-Lactam Antibiotic-Resistance Determinants in Methicillin-Resistant Staphylococcus aureus (MRSA)
title Regulation of the Expression of the β-Lactam Antibiotic-Resistance Determinants in Methicillin-Resistant Staphylococcus aureus (MRSA)
title_full Regulation of the Expression of the β-Lactam Antibiotic-Resistance Determinants in Methicillin-Resistant Staphylococcus aureus (MRSA)
title_fullStr Regulation of the Expression of the β-Lactam Antibiotic-Resistance Determinants in Methicillin-Resistant Staphylococcus aureus (MRSA)
title_full_unstemmed Regulation of the Expression of the β-Lactam Antibiotic-Resistance Determinants in Methicillin-Resistant Staphylococcus aureus (MRSA)
title_short Regulation of the Expression of the β-Lactam Antibiotic-Resistance Determinants in Methicillin-Resistant Staphylococcus aureus (MRSA)
title_sort regulation of the expression of the β-lactam antibiotic-resistance determinants in methicillin-resistant staphylococcus aureus (mrsa)
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3971960/
https://www.ncbi.nlm.nih.gov/pubmed/24564530
http://dx.doi.org/10.1021/bi500074w
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