A mass spectrometry-based hybrid method for structural modelling of protein complexes

We describe a method that integrates data derived from different mass spectrometric (MS) techniques with a modelling strategy for structural characterization of protein assemblies. We encoded structural data derived from native MS, bottom-up proteomics, ion mobility-MS and chemical cross-linking MS...

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Detalles Bibliográficos
Autores principales: Politis, Argyris, Stengel, Florian, Hall, Zoe, Hernández, Helena, Leitner, Alexander, Walzthoeni, Thomas, Robinson, Carol V., Aebersold, Ruedi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3972104/
https://www.ncbi.nlm.nih.gov/pubmed/24509631
http://dx.doi.org/10.1038/nmeth.2841
Descripción
Sumario:We describe a method that integrates data derived from different mass spectrometric (MS) techniques with a modelling strategy for structural characterization of protein assemblies. We encoded structural data derived from native MS, bottom-up proteomics, ion mobility-MS and chemical cross-linking MS into modelling restraints to compute the most likely structure of a protein assembly. We used the method to generate near-native models for three known structures and characterized an assembly intermediate of the proteasomal base.

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