A mass spectrometry-based hybrid method for structural modelling of protein complexes

We describe a method that integrates data derived from different mass spectrometric (MS) techniques with a modelling strategy for structural characterization of protein assemblies. We encoded structural data derived from native MS, bottom-up proteomics, ion mobility-MS and chemical cross-linking MS...

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Detalles Bibliográficos
Autores principales: Politis, Argyris, Stengel, Florian, Hall, Zoe, Hernández, Helena, Leitner, Alexander, Walzthoeni, Thomas, Robinson, Carol V., Aebersold, Ruedi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3972104/
https://www.ncbi.nlm.nih.gov/pubmed/24509631
http://dx.doi.org/10.1038/nmeth.2841
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author Politis, Argyris
Stengel, Florian
Hall, Zoe
Hernández, Helena
Leitner, Alexander
Walzthoeni, Thomas
Robinson, Carol V.
Aebersold, Ruedi
author_facet Politis, Argyris
Stengel, Florian
Hall, Zoe
Hernández, Helena
Leitner, Alexander
Walzthoeni, Thomas
Robinson, Carol V.
Aebersold, Ruedi
author_sort Politis, Argyris
collection PubMed
description We describe a method that integrates data derived from different mass spectrometric (MS) techniques with a modelling strategy for structural characterization of protein assemblies. We encoded structural data derived from native MS, bottom-up proteomics, ion mobility-MS and chemical cross-linking MS into modelling restraints to compute the most likely structure of a protein assembly. We used the method to generate near-native models for three known structures and characterized an assembly intermediate of the proteasomal base.
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spelling pubmed-39721042014-10-01 A mass spectrometry-based hybrid method for structural modelling of protein complexes Politis, Argyris Stengel, Florian Hall, Zoe Hernández, Helena Leitner, Alexander Walzthoeni, Thomas Robinson, Carol V. Aebersold, Ruedi Nat Methods Article We describe a method that integrates data derived from different mass spectrometric (MS) techniques with a modelling strategy for structural characterization of protein assemblies. We encoded structural data derived from native MS, bottom-up proteomics, ion mobility-MS and chemical cross-linking MS into modelling restraints to compute the most likely structure of a protein assembly. We used the method to generate near-native models for three known structures and characterized an assembly intermediate of the proteasomal base. 2014-02-09 2014-04 /pmc/articles/PMC3972104/ /pubmed/24509631 http://dx.doi.org/10.1038/nmeth.2841 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Politis, Argyris
Stengel, Florian
Hall, Zoe
Hernández, Helena
Leitner, Alexander
Walzthoeni, Thomas
Robinson, Carol V.
Aebersold, Ruedi
A mass spectrometry-based hybrid method for structural modelling of protein complexes
title A mass spectrometry-based hybrid method for structural modelling of protein complexes
title_full A mass spectrometry-based hybrid method for structural modelling of protein complexes
title_fullStr A mass spectrometry-based hybrid method for structural modelling of protein complexes
title_full_unstemmed A mass spectrometry-based hybrid method for structural modelling of protein complexes
title_short A mass spectrometry-based hybrid method for structural modelling of protein complexes
title_sort mass spectrometry-based hybrid method for structural modelling of protein complexes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3972104/
https://www.ncbi.nlm.nih.gov/pubmed/24509631
http://dx.doi.org/10.1038/nmeth.2841
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