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Viral Communities Associated with Human Pericardial Fluids in Idiopathic Pericarditis

Pericarditis is a common human disease defined by inflammation of the pericardium. Currently, 40% to 85% of pericarditis cases have no identified etiology. Most of these cases are thought to be caused by an infection of undetected, unsuspected or unknown viruses. In this work, we used a culture- and...

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Autores principales: Fancello, Laura, Monteil, Sonia, Popgeorgiev, Nikolay, Rivet, Romain, Gouriet, Frédérique, Fournier, Pierre-Edouard, Raoult, Didier, Desnues, Christelle
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3972187/
https://www.ncbi.nlm.nih.gov/pubmed/24690743
http://dx.doi.org/10.1371/journal.pone.0093367
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author Fancello, Laura
Monteil, Sonia
Popgeorgiev, Nikolay
Rivet, Romain
Gouriet, Frédérique
Fournier, Pierre-Edouard
Raoult, Didier
Desnues, Christelle
author_facet Fancello, Laura
Monteil, Sonia
Popgeorgiev, Nikolay
Rivet, Romain
Gouriet, Frédérique
Fournier, Pierre-Edouard
Raoult, Didier
Desnues, Christelle
author_sort Fancello, Laura
collection PubMed
description Pericarditis is a common human disease defined by inflammation of the pericardium. Currently, 40% to 85% of pericarditis cases have no identified etiology. Most of these cases are thought to be caused by an infection of undetected, unsuspected or unknown viruses. In this work, we used a culture- and sequence-independent approach to investigate the viral DNA communities present in human pericardial fluids. Seven viral metagenomes were generated from the pericardial fluid of patients affected by pericarditis of unknown etiology and one metagenome was generated from the pericardial fluid of a sudden infant death case. As a positive control we generated one metagenome from the pericardial fluid of a patient affected by pericarditis caused by herpesvirus type 3. Furthermore, we used as negative controls a total of 6 pericardial fluids from 6 different individuals affected by pericarditis of non-infectious origin: 5 of them were sequenced as a unique pool and the remaining one was sequenced separately. The results showed a significant presence of torque teno viruses especially in one patient, while herpesviruses and papillomaviruses were present in the positive control. Co-infections by different genotypes of the same viral type (torque teno viruses) or different viruses (herpesviruses and papillomaviruses) were observed. Sequences related to bacteriophages infecting Staphylococcus, Enterobacteria, Streptococcus, Burkholderia and Pseudomonas were also detected in three patients. This study detected torque teno viruses and papillomaviruses, for the first time, in human pericardial fluids.
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spelling pubmed-39721872014-04-04 Viral Communities Associated with Human Pericardial Fluids in Idiopathic Pericarditis Fancello, Laura Monteil, Sonia Popgeorgiev, Nikolay Rivet, Romain Gouriet, Frédérique Fournier, Pierre-Edouard Raoult, Didier Desnues, Christelle PLoS One Research Article Pericarditis is a common human disease defined by inflammation of the pericardium. Currently, 40% to 85% of pericarditis cases have no identified etiology. Most of these cases are thought to be caused by an infection of undetected, unsuspected or unknown viruses. In this work, we used a culture- and sequence-independent approach to investigate the viral DNA communities present in human pericardial fluids. Seven viral metagenomes were generated from the pericardial fluid of patients affected by pericarditis of unknown etiology and one metagenome was generated from the pericardial fluid of a sudden infant death case. As a positive control we generated one metagenome from the pericardial fluid of a patient affected by pericarditis caused by herpesvirus type 3. Furthermore, we used as negative controls a total of 6 pericardial fluids from 6 different individuals affected by pericarditis of non-infectious origin: 5 of them were sequenced as a unique pool and the remaining one was sequenced separately. The results showed a significant presence of torque teno viruses especially in one patient, while herpesviruses and papillomaviruses were present in the positive control. Co-infections by different genotypes of the same viral type (torque teno viruses) or different viruses (herpesviruses and papillomaviruses) were observed. Sequences related to bacteriophages infecting Staphylococcus, Enterobacteria, Streptococcus, Burkholderia and Pseudomonas were also detected in three patients. This study detected torque teno viruses and papillomaviruses, for the first time, in human pericardial fluids. Public Library of Science 2014-04-01 /pmc/articles/PMC3972187/ /pubmed/24690743 http://dx.doi.org/10.1371/journal.pone.0093367 Text en © 2014 Fancello et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Fancello, Laura
Monteil, Sonia
Popgeorgiev, Nikolay
Rivet, Romain
Gouriet, Frédérique
Fournier, Pierre-Edouard
Raoult, Didier
Desnues, Christelle
Viral Communities Associated with Human Pericardial Fluids in Idiopathic Pericarditis
title Viral Communities Associated with Human Pericardial Fluids in Idiopathic Pericarditis
title_full Viral Communities Associated with Human Pericardial Fluids in Idiopathic Pericarditis
title_fullStr Viral Communities Associated with Human Pericardial Fluids in Idiopathic Pericarditis
title_full_unstemmed Viral Communities Associated with Human Pericardial Fluids in Idiopathic Pericarditis
title_short Viral Communities Associated with Human Pericardial Fluids in Idiopathic Pericarditis
title_sort viral communities associated with human pericardial fluids in idiopathic pericarditis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3972187/
https://www.ncbi.nlm.nih.gov/pubmed/24690743
http://dx.doi.org/10.1371/journal.pone.0093367
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