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Transcription Factor ZBED6 Mediates IGF2 Gene Expression by Regulating Promoter Activity and DNA Methylation in Myoblasts
Zinc finger, BED-type containing 6 (ZBED6) is an important transcription factor in placental mammals, affecting development, cell proliferation and growth. In this study, we found that the expression of the ZBED6 and IGF2 were upregulated during C2C12 differentiation. The IGF2 expression levels were...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3972505/ https://www.ncbi.nlm.nih.gov/pubmed/24691566 http://dx.doi.org/10.1038/srep04570 |
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author | Huang, Yong-Zhen Zhang, Liang-Zhi Lai, Xin-Sheng Li, Ming-Xun Sun, Yu-Jia Li, Cong-Jun Lan, Xian-Yong Lei, Chu-Zhao Zhang, Chun-Lei Zhao, Xin Chen, Hong |
author_facet | Huang, Yong-Zhen Zhang, Liang-Zhi Lai, Xin-Sheng Li, Ming-Xun Sun, Yu-Jia Li, Cong-Jun Lan, Xian-Yong Lei, Chu-Zhao Zhang, Chun-Lei Zhao, Xin Chen, Hong |
author_sort | Huang, Yong-Zhen |
collection | PubMed |
description | Zinc finger, BED-type containing 6 (ZBED6) is an important transcription factor in placental mammals, affecting development, cell proliferation and growth. In this study, we found that the expression of the ZBED6 and IGF2 were upregulated during C2C12 differentiation. The IGF2 expression levels were negatively associated with the methylation status in beef cattle (P < 0.05). A luciferase assay for the IGF2 intron 3 and P3 promoter showed that the mutant-type 439 A-SNP-pGL3 in driving reporter gene transcription is significantly higher than that of the wild-type 439 G-SNP-pGL3 construct (P < 0.05). An over-expression assay revealed that ZBED6 regulate IGF2 expression and promote myoblast differentiation. Furthermore, knockdown of ZBED6 led to IGF2 expression change in vitro. Taken together, these results suggest that ZBED6 inhibits IGF2 activity and expression via a G to A transition disrupts the interaction. Thus, we propose that ZBED6 plays a critical role in myogenic differentiation. |
format | Online Article Text |
id | pubmed-3972505 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-39725052014-04-02 Transcription Factor ZBED6 Mediates IGF2 Gene Expression by Regulating Promoter Activity and DNA Methylation in Myoblasts Huang, Yong-Zhen Zhang, Liang-Zhi Lai, Xin-Sheng Li, Ming-Xun Sun, Yu-Jia Li, Cong-Jun Lan, Xian-Yong Lei, Chu-Zhao Zhang, Chun-Lei Zhao, Xin Chen, Hong Sci Rep Article Zinc finger, BED-type containing 6 (ZBED6) is an important transcription factor in placental mammals, affecting development, cell proliferation and growth. In this study, we found that the expression of the ZBED6 and IGF2 were upregulated during C2C12 differentiation. The IGF2 expression levels were negatively associated with the methylation status in beef cattle (P < 0.05). A luciferase assay for the IGF2 intron 3 and P3 promoter showed that the mutant-type 439 A-SNP-pGL3 in driving reporter gene transcription is significantly higher than that of the wild-type 439 G-SNP-pGL3 construct (P < 0.05). An over-expression assay revealed that ZBED6 regulate IGF2 expression and promote myoblast differentiation. Furthermore, knockdown of ZBED6 led to IGF2 expression change in vitro. Taken together, these results suggest that ZBED6 inhibits IGF2 activity and expression via a G to A transition disrupts the interaction. Thus, we propose that ZBED6 plays a critical role in myogenic differentiation. Nature Publishing Group 2014-04-02 /pmc/articles/PMC3972505/ /pubmed/24691566 http://dx.doi.org/10.1038/srep04570 Text en Copyright © 2014, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-nd/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. The images in this article are included in the article's Creative Commons license, unless indicated otherwise in the image credit; if the image is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the image. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/ |
spellingShingle | Article Huang, Yong-Zhen Zhang, Liang-Zhi Lai, Xin-Sheng Li, Ming-Xun Sun, Yu-Jia Li, Cong-Jun Lan, Xian-Yong Lei, Chu-Zhao Zhang, Chun-Lei Zhao, Xin Chen, Hong Transcription Factor ZBED6 Mediates IGF2 Gene Expression by Regulating Promoter Activity and DNA Methylation in Myoblasts |
title | Transcription Factor ZBED6 Mediates IGF2 Gene Expression by Regulating Promoter Activity and DNA Methylation in Myoblasts |
title_full | Transcription Factor ZBED6 Mediates IGF2 Gene Expression by Regulating Promoter Activity and DNA Methylation in Myoblasts |
title_fullStr | Transcription Factor ZBED6 Mediates IGF2 Gene Expression by Regulating Promoter Activity and DNA Methylation in Myoblasts |
title_full_unstemmed | Transcription Factor ZBED6 Mediates IGF2 Gene Expression by Regulating Promoter Activity and DNA Methylation in Myoblasts |
title_short | Transcription Factor ZBED6 Mediates IGF2 Gene Expression by Regulating Promoter Activity and DNA Methylation in Myoblasts |
title_sort | transcription factor zbed6 mediates igf2 gene expression by regulating promoter activity and dna methylation in myoblasts |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3972505/ https://www.ncbi.nlm.nih.gov/pubmed/24691566 http://dx.doi.org/10.1038/srep04570 |
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